Information on EC 3.6.1.B16 - guanosine-3',5'-bis(diphosphate) bisphosphatase

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The expected taxonomic range for this enzyme is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
3.6.1.B16
preliminary BRENDA-supplied Ec number
RECOMMENDED NAME
GeneOntology No.
guanosine-3',5'-bis(diphosphate) bisphosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
guanosine-3',5'-bis(diphosphate) + H2O = guanosine-3',5'-bisphosphate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
guanosine-3',5'-bis(diphosphate)-3',5'-bisphosphohydrolase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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T-DNA insertion mutants do not exhibit any phenotypical differences under normal growth conditions. The gene NUDX26 cannot complement the mutator phenotype of the Escherichia coli mutT- strain
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
AppppA + H2O
?
show the reaction diagram
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-
-
-
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ApppppA + H2O
?
show the reaction diagram
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-
-
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guanosine 3',5'-bis(diphosphate) + 2 H2O
guanosine 3',5'-bisphoshate + 2 phosphate
show the reaction diagram
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enzyme has diphosphohydrolase activity towards both diphosphate linkages in ppGpp and generates pGp as final product
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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or Mn2+, absolutely required
Mn2+
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or Mg2+, absolutely required
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
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activity increases in presence of 1 mM dithiothreitol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0218
AppppA
-
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
0.0096
ApppppA
-
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
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0.135
Guanosine 3',5'-bis(diphosphate)
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pH 8.0, 37°C, presence of 5 mM Mg2+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16.9
AppppA
Arabidopsis thaliana
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pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
11.8
ApppppA
Arabidopsis thaliana
-
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
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0.49
Guanosine 3',5'-bis(diphosphate)
Arabidopsis thaliana
-
pH 8.0, 37°C, presence of 5 mM Mg2+
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
770
AppppA
Arabidopsis thaliana
-
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
105466
1200
ApppppA
Arabidopsis thaliana
-
pH 8.0, 37°C, presence of 5 mM Mg2+, 1 mM dithiothreitol
197746
3.6
Guanosine 3',5'-bis(diphosphate)
Arabidopsis thaliana
-
pH 8.0, 37°C, presence of 5 mM Mg2+
46041
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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50% of maximum activity
9.5
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50% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of isoform NUDX26 decreases upon treatment with paraquat and under salinity stress
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