Information on EC 3.6.1.52 - diphosphoinositol-polyphosphate diphosphatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.6.1.52
-
RECOMMENDED NAME
GeneOntology No.
diphosphoinositol-polyphosphate diphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
diphospho-myo-inositol-polyphosphate diphosphohydrolase
This enzyme hydrolyses the diphosphate bond, leaving a phospho group where a diphospho group had been. It can also act on bis(adenosine) diphosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
220324-74-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-phospho-alpha-D-ribose 1-diphosphate + H2O
5-phospho-alpha-D-ribose 1-phosphate + phosphate
show the reaction diagram
8-oxo-dATP + H2O
?
show the reaction diagram
-
low activity
-
-
?
8-oxo-dGTP + H2O
?
show the reaction diagram
-
low activity
-
-
?
adenosine tetraphosphate + H2O
?
show the reaction diagram
-
the substrate shows great hydrolysis by Aps
-
-
?
ADP-glucose + H2O
?
show the reaction diagram
-
low activity
-
-
?
ADP-ribose + H2O
?
show the reaction diagram
-
low activity
-
-
?
bisdiphosphoinositol tetrakisphosphate + H2O
? + phosphate
show the reaction diagram
bisdiphosphoinositol tetrakisphosphate + H2O
diphosphoinositol pentakisphosphate + phosphate
show the reaction diagram
CTP + H2O
?
show the reaction diagram
-
low activity
-
-
?
dATP + H2O
dADP + phosphate
show the reaction diagram
-
-
-
-
diadenosine 5',5'''-P1,P5-pentaphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
diadenosine 5',5'''-P1,P6-hexaphosphate + H2O
?
show the reaction diagram
-
the substrate shows great hydrolysis by Aps
-
-
?
diadenosine 5',5''-P1,P6-hexaphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
diguanosine pentaphosphate + H2O
?
show the reaction diagram
-
low activity
-
-
?
diphosphoinositol pentakisphosphate + H2O
? + phosphate
show the reaction diagram
-
-
-
-
?
diphosphoinositol pentakisphosphate + H2O
inositol hexakisphosphate + phosphate
show the reaction diagram
diphosphoinositol pentakisphosphate + H2O
inositol pentakisphosphate + phosphate
show the reaction diagram
diphosphoinositol polyphosphate + H2O
inositol polyphosphate + phosphate
show the reaction diagram
dTTP + H2O
?
show the reaction diagram
-
low activity
-
-
?
dUTP + H2O
?
show the reaction diagram
-
low activity
-
-
?
GDP-glucose + H2O
?
show the reaction diagram
-
low activity
-
-
?
GDP-mannose + H2O
?
show the reaction diagram
-
low activity
-
-
?
guanosine tetraphosphate + H2O
?
show the reaction diagram
-
the substrate shows the greatest hydrolysis by Aps
-
-
?
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
show the reaction diagram
-
reaction of EC 3.6.1.17
-
?
TDP-glucose + H2O
?
show the reaction diagram
-
low activity
-
-
?
UDP-glucose + H2O
?
show the reaction diagram
-
low activity
-
-
?
UDP-N-acetyl-D-galactosamine + H2O
?
show the reaction diagram
-
low activity
-
-
?
UDP-N-acetyl-D-glucosamine + H2O
?
show the reaction diagram
-
low activity
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
diphosphoinositol polyphosphate + H2O
inositol polyphosphate + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
Co2+ supports less than 5% of the activity with Mg2+
additional information
-
Ca2+, Ni2+ or Zn2+ are ineffective in stimulating activity of Aps
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
F-
-
uncompetitive against bisdiphosphoinositol tetrakisphosphate, non-competitive against diphosphoinositol pentakisphosphate
inositol 1-[(phosphorylmethyl)phosphonic acid]-2,3,4,5,6-pentaphosphate
competitive
inositol 1-[(phosphorylmethyl)phosphonic acid]-2,3,5,6-tetraphosphate
competitive
inositol 4-butyl-1-[(phosphorylmethyl)phosphonic acid]-2,3,5,6-tetraphosphate
competitive
Inositol hexakisphosphate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13
5-phospho-D-ribosyl 1-diphosphate
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
0.004
adenosine tetraphosphate
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
0.000034
bisdiphosphoinositol tetrakisphosphate
-
-
0.018
diadenosine 5',5'''-P1,P6-hexaphosphate
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
0.0000042 - 0.0012
diphosphoinositol pentakisphosphate
0.011
guanosine tetraphosphate
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
additional information
additional information
-
Km-values for hydrolysis of Ap6A and Ap5A
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1
5-phospho-D-ribosyl 1-diphosphate
Drosophila melanogaster
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
0.24
adenosine tetraphosphate
Drosophila melanogaster
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
0.34
diadenosine 5',5'''-P1,P6-hexaphosphate
Drosophila melanogaster
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
0.024 - 0.2
diphosphoinositol pentakisphosphate
0.8
guanosine tetraphosphate
Drosophila melanogaster
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
additional information
additional information
Homo sapiens
-
turnover-numbers for hydrolysis of Ap6A and Ap5A
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
5-phospho-D-ribosyl 1-diphosphate
Drosophila melanogaster
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
36846
60
adenosine tetraphosphate
Drosophila melanogaster
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
10567
19
diadenosine 5',5'''-P1,P6-hexaphosphate
Drosophila melanogaster
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
32199
342
diphosphoinositol pentakisphosphate
Drosophila melanogaster
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
3013
71
guanosine tetraphosphate
Drosophila melanogaster
-
in the presence of 10 mM Mg2+, at pH 9.0 and 26C
15776
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0084 - 0.0109
F-
0.00011
inositol 1-[(phosphorylmethyl)phosphonic acid]-2,3,4,5,6-pentaphosphate
pH not specified in the publication, temperature not specified in the publication
0.00027
inositol 1-[(phosphorylmethyl)phosphonic acid]-2,3,5,6-tetraphosphate
pH not specified in the publication, temperature not specified in the publication
0.000023
inositol 4-butyl-1-[(phosphorylmethyl)phosphonic acid]-2,3,5,6-tetraphosphate
pH not specified in the publication, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
-
hydrolysis of diphosphoinositol pentakisphosphate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
-
pH 6.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity, hydrolysis of diphosphoinositol pentakisphosphate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26
-
at 10 mM Mg2+
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
Aps is predominantly cytoplasmic
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
-
gel filtration
19000
-
SDS PAGE
19500
-
x * 19500, calculation from nucleotide sequence
20434
-
x * 20434, calculation from nucleotide sequence
23000
native enzyme, Western-blot analysis
30000
-
recombinant enzyme, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no modification
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
activity is 5% of that at pH 9.5
659817
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dithiothreitol
-
in the absence of dithiothreitol a 5 min pretreatment at 36C lowers enzyme activity by 65%, with temperatures above 56C virtually abolishing all activity, whereas in the presence of dithiothreitol a 5 min treatment at temperatures up to 76C is without effect
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
heat lability is restored by H2O2
-
712171
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
amylose resin chromatography
-
by nickel-agarose affinity chromatography
Ni-NTA column chromatography
-
recombinant enzyme, by nickel-nitrilotriacetic acid-agarose chromatography
-
recombinant enzyme, by TALON metal affinity chromatography; recombinant enzyme, by TALON metal affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli M15 cells as a hexahistidine-tagged fusion protein
-
expressed in Escherichia coli strain SBMutT- and TX48
-
expression in Epicurian coli
expression in Escherichia coli
isoenzyme hDIPP2alpha and hDIPP2beta
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Aps expression is highest in embryos and declines throughout development
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E66Q
-
no significant activity towards diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate, activity with diadenosine 5',5'''-P1,P6-hexaphosphate is 2% of the activity of the wild-type enzyme
F84Y
-
hydrolysis of bisdiphosphoinositol tetrakisphosphate at 63% of the activity of the wild-type enzyme, hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate is 75% of the activity of the wild-type enzyme, activity with diphosphoinositol pentakisphosphate is only slightly reduced
G50A
-
hydrolysis of diphosphoinositol polyphosphates is more than 85% less than that of the wild-type enzyme
G52A
-
hydrolysis of diphosphoinositol polyphosphates is more than 85% less than that of the wild-type enzyme
G72A
-
catalytic activity towards bisdiphosphoinositol tetrakisphosphate, diadenosine 5',5'''-P1,P6-hexaphosphateand diphosphoinositol pentakisphosphate is impaired by more than 95%
G75A
-
catalytic activity towards bisdiphosphoinositol tetrakisphosphate, diadenosine 5',5'''-P1,P6-hexaphosphateand diphosphoinositol pentakisphosphate is impaired by more than 95%
G78A
-
catalytic activity and CD spectrum is not significantly different from that of the wild.type enzyme
G82A
-
mutation promotes some unraveling of the normal secondary structure
H91L
-
hydrolysis of bisdiphosphoinositol tetrakisphosphate at 4% of the activity of the wild-type enzyme, hydrolysis of diadenosine 5',5'''-P1,P6-hexaphosphate is 26% of the activity of the wild-type enzyme, activity with diphosphoinositol pentakisphosphate is only slightly reduced
P89R
codes for isoenzyme hDIPP3beta
E70Q
-
greatly reduced rate of hydrolysis of diphosphoinositol pentakisphosphate or bisdiphosphoinositol tetrakisphosphate