Information on EC 3.6.1.43 - dolichyldiphosphatase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.6.1.43
-
RECOMMENDED NAME
GeneOntology No.
dolichyldiphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dolichyl diphosphate + H2O = dolichyl phosphate + phosphate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of diphosphate bonds
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
N-Glycan biosynthesis
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-
dolichol and dolichyl phosphate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dolichyl-diphosphate phosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
73361-26-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
calf
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
DLPP1 gene
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
olicholpyrophosphatase participates in glycoprotein biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + H2O
?
show the reaction diagram
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-
-
-
?
ATP + H2O
?
show the reaction diagram
-
-
-
-
?
diphosphate + H2O
phosphate
show the reaction diagram
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-
-
-
?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
show the reaction diagram
dolichyl phosphate + H2O
dolichol + phosphate
show the reaction diagram
-
-
-
-
?
geranyldiphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
isopentenyldiphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
enzyme can not hydrolyse dolichyl monophospate
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
inhibitory
Mn2+
-
strong inhibitor
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bacitracin
Ca2+
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Divalent cations
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dolichol
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farnesol
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very slight inhibition
farnesyl diphosphate
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-
isopentenyl diphosphate
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-
Mn2+
-
strong inhibitor
phosphate
phosphatidic acid
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70% inhibition at 60 mM
phosphatidylcholine
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nearly full inhibition
phosphatidylethanolamine
-
-
Triton X-100
undecaprenyl diphosphate
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-
additional information
-
bacitracin fails to inhibit the acid PPase
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diphosphate
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phosphate
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Trimetaphosphate
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Triton X-100
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optimum activity above 0.14%, stable up to 4.2%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02 - 0.166
dolichyl diphosphate
0.048
dolichyl phosphate
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pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.003
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day 4 of embryonic development
0.0031
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0.005
pH 7, 30°C, Dol-P-P phosphatase activity using microsomal fractions from Sf-9 cells expressing the enzyme
0.016
pH 7, 30°C, Dol-P-P phosphatase activity using microsomal fractions from COS cells expressing the enzyme
0.032
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day 22 of embryonic development
additional information
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increases significantly during day 12-25 of embryonic development
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
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in the presence of octyl glucoside
7
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optimum activity at neutral pH
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
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95% and 90% of activity, broad pH-range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
RPMI 4265
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
absent in peroxisomes and inner mitochondrial membranes
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Manually annotated by BRENDA team
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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2 min causes 80-85% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the enzyme is labile to Triton X-100 when expressed in Sf-9 cells
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C solubilized enzyme can be stored for 6 months without loss of activity
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-17°C stable one month
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4°C solubilized enzyme stable overnight
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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overexpression in Saccharomyces cerevisiae and Sf-9 insect cells
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overexpression in Saccharomyces cerevisiae, COS cells and Sf-9 insect cells
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
lipids from the Sulfolobus membranes enhance the activity of the acid PPase to about 3fold
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