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EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Synonyms
acid pyrophosphatase, dol-p-p phosphatase, dolichyl-diphosphate phosphatase, dppase, dolichyl pyrophosphate phosphatase, dolichyl pyrophosphatase,
more
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dol-P-P phosphohydrolase
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dolichol diphosphatase
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Dolicholpyrophosphatase
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dolichyl diphosphate phosphohydrolase
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dolichyl pyrophosphatase
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dolichyl pyrophosphate phosphatase
dolichyl-diphosphate phosphatase
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CAX4
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Dol-P-P phosphatase
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Dol-PP phosphatase
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dolichyl pyrophosphate phosphatase
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dolichyl pyrophosphate phosphatase
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dolichyl pyrophosphate phosphatase
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dolichyl pyrophosphate phosphatase
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dolichyl diphosphate + H2O = dolichyl phosphate + phosphate
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hydrolysis of diphosphate bonds
hydrolysis of diphosphate bonds
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hydrolysis of diphosphate bonds
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hydrolysis of diphosphate bonds
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dolichyl-diphosphate phosphohydrolase
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diphosphate + H2O
phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
dolichyl phosphate + H2O
dolichol + phosphate
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?
geranyldiphosphate + H2O
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?
isopentenyldiphosphate + H2O
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?
additional information
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dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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Dol-P produced by DPPase contributes significantly to the pool of Dol-P utilized for lipid intermediate biosynthesis on the cytoplasmic leaflet, dolichyl phosphate metabolism, overview
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dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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calf brain microsomes, sealed vesicles, are used as substrates
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dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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additional information
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enzyme can not hydrolyse dolichyl monophospate
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additional information
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enzyme can not hydrolyse dolichyl monophospate
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?
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dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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involved in biosynthesis of glycoproteins, regeneration of dolichyl phosphate
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?
dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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Dol-P produced by DPPase contributes significantly to the pool of Dol-P utilized for lipid intermediate biosynthesis on the cytoplasmic leaflet, dolichyl phosphate metabolism, overview
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dolichyl diphosphate + H2O
dolichyl phosphate + phosphate
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?
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additional information
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no metal ions required
additional information
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no metal ions required
additional information
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inhibited by divalent cations
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farnesol
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very slight inhibition
isopentenyl diphosphate
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phosphatidic acid
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70% inhibition at 60 mM
phosphatidylcholine
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nearly full inhibition
phosphatidylethanolamine
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undecaprenyl diphosphate
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additional information
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bacitracin fails to inhibit the acid PPase
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bacitracin
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complete inhibition
bacitracin
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no inhibition when preincubating with EDTA
bacitracin
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complete inhibition; no inhibition when preincubating with EDTA
divalent cations
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divalent cations
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strong or complete inhibition at concentrations of 5 mM for all tested ions
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NaF
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slight inhibition
phosphate
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activating
phosphate
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slight inhibition
phosphate
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strong inhibition, 5 mM causes more than 60% loss of activity
Triton X-100
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activating
Triton X-100
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above 0.2%
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Triton X-100
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optimum activity above 0.14%, stable up to 4.2%
EDTA
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Influenza, Human
Characterization and comparison of the full 3' and 5' untranslated genomic regions of diverse isolates of infectious salmon anaemia virus by using a rapid and universal method.
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0.02 - 0.166
dolichyl diphosphate
0.048
dolichyl phosphate
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pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
0.02
dolichyl diphosphate
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0.025
dolichyl diphosphate
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day 4 of embryonic development
0.04
dolichyl diphosphate
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pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
0.166
dolichyl diphosphate
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day 22 of embryonic development
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0.003
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day 4 of embryonic development
0.005
pH 7, 30°C, Dol-P-P phosphatase activity using microsomal fractions from Sf-9 cells expressing the enzyme
0.016
pH 7, 30°C, Dol-P-P phosphatase activity using microsomal fractions from COS cells expressing the enzyme
0.032
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day 22 of embryonic development
additional information
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increases significantly during day 12-25 of embryonic development
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6 - 7
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in the presence of octyl glucoside
7
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optimum activity at neutral pH
8
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7 - 9
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95% and 90% of activity, broad pH-range
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calf
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brenda
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brenda
DLPP1 gene
SwissProt
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brenda
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brenda
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brenda
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brenda
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RPMI 4265
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embryonic
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brenda
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additional information
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absent in peroxisomes and inner mitochondrial membranes
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brenda
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brenda
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transmembrane protein with a luminally oriented active site
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integral
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brenda
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integral
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plasma, outer mitochondrial membranes
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malfunction
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in enzyme-deficient cells showing endoplasmic reticulum stress, membrane and growth defect, the N-glycosylation of the vacuolar carboxypeptidase is severely affected, and expression of the endoplasmic reticulum chaperone Kar2p is elevated, which results in unfolded protein response activation as an adaptive response
malfunction
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in enzyme-deficient cells showing endoplasmic reticulum stress, membrane and growth defect, the N-glycosylation of the vacuolar carboxypeptidase is severely affected, and expression of the endoplasmic reticulum chaperone Kar2p is elevated, which results in unfolded protein response activation as an adaptive response
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metabolism
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olicholpyrophosphatase participates in glycoprotein biosynthesis
metabolism
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the enzyme is essential for phospholipid homeostasis
metabolism
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the enzyme is essential for phospholipid homeostasis
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physiological function
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the enzyme regulates membrane biogenesis by coordinating lipid homeostasis with protein quality control
physiological function
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the enzyme regulates membrane biogenesis by coordinating lipid homeostasis with protein quality control
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50
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2 min causes 80-85% loss of activity
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the enzyme is labile to Triton X-100 when expressed in Sf-9 cells
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-10°C solubilized enzyme can be stored for 6 months without loss of activity
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4°C solubilized enzyme stable overnight
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expressed in Escherichia coli
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overexpression in Saccharomyces cerevisiae and Sf-9 insect cells
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overexpression in Saccharomyces cerevisiae, COS cells and Sf-9 insect cells
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lipids from the Sulfolobus membranes enhance the activity of the acid PPase to about 3fold
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Wedgwood, J.F.; Strominger, J.L.
Enzymatic activities in cultured human lymphocytes that dephosphorylate dolichyl pyrophosphate and dolichyl phosphate
J. Biol. Chem.
255
1120-1123
1980
Homo sapiens
brenda
Belocopitow, E.; Boscoboinik, D.
Dolichyl-phosphate phosphatase and dolichyl-diphosphate phosphatase in rat-liver microsomes
Eur. J. Biochem.
125
167-173
1982
Rattus norvegicus
brenda
Bhat, N.R.; Frank, D.W.; Wolf, M.J.; Waechter, C.J.
Developmental changes in enzymes involved in dolichyl phosphate metabolism in cultured embryonic rat brain cells
J. Neurochem.
56
339-344
1991
Rattus norvegicus
brenda
Applekvist, E.L.; Chojnacki, T.; Dallner, G.
Dolichol (C55) mono-and pyrophosphatases of the rat liver
Biosci. Rep.
1
619-626
1981
Rattus norvegicus
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brenda
Boscoboinik, D.O.; Belocopitow, E.
Assay of dolichol monophosphate and dolichol diphosphate phosphatases: thin-layer chromatography in a nonequilibrated system
Anal. Biochem.
114
42-45
1981
Rattus norvegicus
brenda
Scher, M.G.; Waechter, C.J.
Brain dolichyl pyrophosphate phosphatase. Solubilization, characterization, and differentiation from dolichyl monophosphate phosphatase activity
J. Biol. Chem.
259
14580-14585
1984
Bos taurus
brenda
Scher, M.G.; Waechter, C.J.
Dolichyl pyrophosphate phosphatase in brain
Methods Enzymol.
111
547-553
1985
Bos taurus
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Shabalin, Y.A.; Hamut, A.; Bukhtiyarov, Y.E.; Kulaev, I.S.
Intracellular distribution and solubilization of yeast dolichylpyrophoshatase
Biochemistry (Moscow)
56
2032-2041
1991
Saccharomyces cerevisiae
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Fernandez, F.; Rush, J.S.; Toke, D.A.; Han, G.S.; Quinn, J.E.; Carman, G.M.; Choi, J.Y.; Voelker, D.R.; Aebi, M.; Waechter, C.J.
The CWH8 gene encodes a dolichyl pyrophosphate phosphatase with a luminally oriented active site in the endoplasmic reticulum of Saccharomyces cerevisiae
J. Biol. Chem.
276
41455-41464
2001
Saccharomyces cerevisiae
brenda
Rush, J.S.; Cho, S.K.; Jiang, S.; Hofmann, S.L.; Waechter, C.J.
Identification and characterization of a cDNA encoding a dolichyl pyrophosphate phosphatase located in the endoplasmic reticulum of mammalian cells
J. Biol. Chem.
277
45226-45234
2002
Mus musculus (Q9JMF7), Mus musculus
brenda
Rush, J.S.; Gao, N.; Lehrman, M.A.; Waechter, C.J.
Recycling of dolichyl monophosphate to the cytoplasmic leaflet of the endoplasmic reticulum after the cleavage of dolichyl pyrophosphate on the lumenal monolayer
J. Biol. Chem.
283
4087-4093
2008
Saccharomyces cerevisiae
brenda
Manabe, F.; Itoh, Y.H.; Shoun, H.; Wakagi, T.
Membrane-bound acid pyrophosphatase from Sulfolobus tokodaii, a thermoacidophilic archaeon: heterologous expression of the gene and characterization of the product
Extremophiles
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859-865
2009
Sulfurisphaera tokodaii
brenda
James, A.W.; Gowsalya, R.; Nachiappan, V.
Dolichyl pyrophosphate phosphatase-mediated N-glycosylation defect dysregulates lipid homeostasis in Saccharomyces cerevisiae
Biochim. Biophys. Acta
1861
1705-1718
2016
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
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