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Information on EC 3.6.1.42 - guanosine-diphosphatase and Organism(s) Candida albicans and UniProt Accession Q8TGH6

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EC Tree
     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.42 guanosine-diphosphatase
IUBMB Comments
Also acts on UDP but not on other nucleoside diphosphates and triphosphates.
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This record set is specific for:
Candida albicans
UNIPROT: Q8TGH6
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Word Map
The taxonomic range for the selected organisms is: Candida albicans
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
gdap1, e-ntpdase, ecto-nucleoside triphosphate diphosphohydrolase, udpase, guanosine diphosphatase, gdp1p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
gda1p (GDA1 gene product)
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-
-
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GDPase
guanosine diphosphatase
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phosphatase, guanosine di-
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-
-
-
UDPase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of diphosphate bonds
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SYSTEMATIC NAME
IUBMB Comments
GDP phosphohydrolase
Also acts on UDP but not on other nucleoside diphosphates and triphosphates.
CAS REGISTRY NUMBER
COMMENTARY hide
98037-56-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + H2O
AMP + phosphate
show the reaction diagram
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-
-
?
GDP + H2O
GMP + phosphate
show the reaction diagram
UDP + H2O
UMP + phosphate
show the reaction diagram
-
-
-
?
GDP + H2O
GMP + phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
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physiological function, role in O- and N-glycosylation, and cell and hyphae morphogenesis, affects the cell surface charge and phosphate content, enzyme is not required for the adhesion of Candida albicans to epithelial cells, overview
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP + H2O
GMP + phosphate
show the reaction diagram
GDP hydrolysis occurs in the Golgi lumen to give GMP, which is required as antiporter for GDP-mannose transport into the Golgi lumen
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-
?
GDP + H2O
GMP + phosphate
show the reaction diagram
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-
-
-
?
additional information
?
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physiological function, role in O- and N-glycosylation, and cell and hyphae morphogenesis, affects the cell surface charge and phosphate content, enzyme is not required for the adhesion of Candida albicans to epithelial cells, overview
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
stimulates
Mn2+
stimulates, preferred metal ion
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene gda1
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
enzyme contains a unique sequence in the stem region predicted with high probability to adopt a coil-coil conformation that favors dimerization
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
disruption of gene gda1 leads to a mutant with 90% reduced GDP hydrolysis activity compared to the wild-type, the mutant also shows severely impaired O-mannosylation and reduced cell wall phosphate content and other phenotypes, but the formation of hyphae is uneffected, overview
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, functional complementation of a Saccharomyces GDPase null mutant
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme activity significantly decreases at high concentrations of menadione, H2O2 and O2-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Herrero, A.B.; Uccelletti, D.; Hirschberg, C.B.; Dominguez, A.; Abeijon, C.
The Golgi GDPase of the fungal pathogen Candida albicans affects morphogenesis, glycosylation, and cell wall properties
Eukaryot. Cell
1
420-431
2002
Candida albicans (Q8TGH6)
Manually annotated by BRENDA team
Delgado-Carmona, J.D.; Ramirez-Quijas, M.D.; Vega-Gonzalez, A.; Lopez-Romero, E.; Cuellar-Cruz, M.
Changes in GDPase/UDPase enzymatic activity in response to oxidative stress in four Candida species
Folia Microbiol. (Praha)
60
343-350
2015
Candida albicans, Candida parapsilosis, Pichia kudriavzevii, [Candida] glabrata
Manually annotated by BRENDA team