Information on EC 3.6.1.31 - phosphoribosyl-ATP diphosphatase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.6.1.31
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RECOMMENDED NAME
GeneOntology No.
phosphoribosyl-ATP diphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Histidine metabolism
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L-histidine biosynthesis
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Metabolic pathways
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histidine metabolism
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SYSTEMATIC NAME
IUBMB Comments
1-(5-phosphoribosyl)-ATP diphosphohydrolase
The Neurospora crassa enzyme also catalyses the reactions of EC 1.1.1.23 (histidinol dehydrogenase) and EC 3.5.4.19 (phosphoribosyl-AMP cyclohydrolase).
CAS REGISTRY NUMBER
COMMENTARY hide
69553-55-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
gene hisE
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-(5-phosphoribosyl)-ATP + H2O
1-(5-phosphoribosyl)-AMP + diphosphate
show the reaction diagram
N-1-(5'-phosphoribosyl)-ATP + H2O
N-1-(5'-phosphoribosyl)-AMP + diphosphate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-(5-phosphoribosyl)-ATP + H2O
1-(5-phosphoribosyl)-AMP + diphosphate
show the reaction diagram
N-1-(5'-phosphoribosyl)-ATP + H2O
N-1-(5'-phosphoribosyl)-AMP + diphosphate
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.022
N-1-(5'-phosphoribosyl)-ATP
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
332
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purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
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75% of maximum activity at pH 7, 79% of maximum activity at pH 10
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711)
Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31670
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calculated from amino acid sequence
43000
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sucrose density gradient centrifugation
95000
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SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 95000, gel filtration, SDS-PAGE
homodimer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native and selenomethionine-labeled, uncomplexed enzyme in two crystal forms, hanging-drop vapor diffusion method, 10 mg/ml protein in 20 mM HEPES, pH 7.5, mixing of protein solution and reservoir buffer containing 100 mM sodium cacodylate pH 6.5, 1.0 M sodium citrate, 0.1 M guanidinium hydrochloride in a 1:1 ratio, 4 days, X-ray structure determination and analysis at 1.25 A and 1.79 A resolution, respectively
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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