Information on EC 3.6.1.29 - bis(5'-adenosyl)-triphosphatase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.6.1.29
-
RECOMMENDED NAME
GeneOntology No.
bis(5'-adenosyl)-triphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP
show the reaction diagram
-
-
-
-
P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP
show the reaction diagram
two-step reaction mechanism via a fragile histidine triad Fhit-AMP intermediate and release of MgADP-, kinetic of the reaction steps, overview
-
P1,P3-bis(5'-adenosyl) triphosphate + H2O = ADP + AMP
show the reaction diagram
the dimeric enzyme contains a catalytic histidine triad with H96 forming a covalent intermediate at the alpha-phosphate of P1,P3-bis(5'-adenosyl)triphosphate, S128, H96 and H98 are essential for the reaction, structure overview
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis
-
-
-
-
hydrolysis
-
-
PATHWAY
KEGG Link
MetaCyc Link
Purine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
P1,P3-bis(5'-adenosyl)-triphosphate adenylohydrolase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ap3A hydrolase
-
-
-
-
Ap3A hydrolase
Q4KTA6
-
Ap3A hydrolase
-
-
Ap3Aase
-
-
-
-
covalent adenylyl enzyme
-
-
Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
-
-
-
-
diadenosine 5,5-P1,P3-triphosphatase
-
-
-
-
diadenosine triphosphatase
-
-
dinucleoside 5',5'''-P1,P3-triphosphate hydrolase
-
-
dinucleoside triphosphatase
Q9LVM4
-
dinucleoside triphosphatase
-
-
dinucleoside-triphosphatase
-
-
dinucleosidetriphosphatase
-
-
-
-
Fhit
Q4KTA6
-
Fhit
P49789
-
Fhit protein
Q9LVM4
-
Fhit protein
-
-
FHIT protein/dinucleoside triphosphatase
-
-
FHIT protein/dinucleoside triphosphatase
Trypanosoma brucei 427
-
-
-
fragile histidine triad
-
-
fragile histidine triad
Q4KTA6
-
fragile histidine triad
P49789
-
Fragile histidine triad protein
-
-
-
-
Fragile histidine triad protein
-
-
fragile HIT protein
P49789
-
HIT-45 protein
-
-
HIT-45 protein
Trypanosoma brucei 427
-
-
-
tumour suppressor Fhit protein
-
-
CAS REGISTRY NUMBER
COMMENTARY
63951-94-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
gene FHIT
UniProt
Manually annotated by BRENDA team
shrimp
-
-
Manually annotated by BRENDA team
gene FHIT fragment; gene FHIT
SwissProt
Manually annotated by BRENDA team
chinese cancer patients
-
-
Manually annotated by BRENDA team
gene FHIT
-
-
Manually annotated by BRENDA team
Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
Trypanosoma brucei 427
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
a loss of FHIT expression associated with poor prognosis and beta-catenin expression
physiological function
-
the FHIT protein is a tumor suppressor. FHIT/ferrodoxin reductase complex generates reactive oxygen species and increases calcium uptake into the mitochondria to potentiate the effects of apoptotic agents
physiological function
-
formation of Fhit-substrate complex results in an apoptosis initiation signal while subsequent hydrolysis of ApnA terminates this action
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine 5'-phosphoimidazolide + UDP
imidazole + dA(5')p3(5')U
show the reaction diagram
-
H96G mutant
-
-
?
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
100% initial velocity of degradationin 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
-
-
?
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
64% initial velocity of degradation
-
-
?
5'-O-[amino(hydroxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
96% initial velocity of degradation
-
-
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
19% initial velocity of degradation
-
-
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
25% initial velocity of degradation
-
-
?
5'-O-[fluoro(hydroxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
97% initial velocity of degradation
-
-
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
100% initial velocity of degradation
-
-
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
48% initial velocity of degradation
-
-
?
5'-O-[hydroxy(sulfooxy)phosphoryl]adenosine + H2O
?
show the reaction diagram
-
65% initial velocity of degradation
-
-
?
7-benzylguanosine(5')triphospho(5')adenosine + H2O
7-benzylguanosine(5')monophosphate + 7-benzylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
7-ethylguanosine(5')triphospho(5')adenosine + H2O
7-ethylguanosine(5')monophosphate + 7-ethylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine(5')triphospho(5')7-methylguanosine + H2O
7-methylguanosine(5')monophosphate + 7-methylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine(5')triphospho(5')adenosine + H2O
7-methylguanosine(5')monophosphate + ADP + AMP + 7-methylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine(5')triphospho(5')adenosine + H2O
7-methylguanosine(5')monophosphate + ADP + AMP + 7-methylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine(5')triphospho(5')cytosine + H2O
7-methylguanosine(5')monophosphate + CDP + CMP + 7-methylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
7-methylguanosine(5')triphospho(5')guanosine + H2O
7-methylguanosine(5')monophosphate + GDP + 7-methylguanosine(5')diphosphate + GMP
show the reaction diagram
-
-
-
-
?
7-methylguanosine(5')triphospho(5')guanosine + H2O
7-methylguanosine(5')monophosphate + GDP + 7-methylguanosine(5')diphosphate + GMP
show the reaction diagram
-
-
-
-
?
7-methylguanosine(5')triphospho(5')uracil + H2O
7-methylguanosine(5')monophosphate + UDP + UMP + 7-methylguanosine(5')diphosphate
show the reaction diagram
-
-
-
-
?
adenosine 5'-phosphoimidazolide + ADP
imidazole + A(5')p3(5')A
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + ATP
imidazole + A(5')p4(5')A
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + CDP
imidazole + A(5')p3(5')C
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + GDP
imidazole + A(5')p3(5')G
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + TDP
imidazole + A(5')p3(5')T
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + UDP
imidazole + A(5')p3(5')U
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoimidazolide + UTP
imidazole + A(5')p4(5')U
show the reaction diagram
-
H96G mutant
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
show the reaction diagram
-
-, preferred substrate
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
show the reaction diagram
Q9LVM4
preferred substrate, no requirement for Mg2+
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
show the reaction diagram
Q9LVM4
no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
show the reaction diagram
-
reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
show the reaction diagram
Q9LVM4
reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenosine-5'-phospho-N-methylimidazolide + H2O
?
show the reaction diagram
-
-
-
-
?
adenosine-5'-phosphoimidazolide + H2O
?
show the reaction diagram
-
-
-
-
?
adenosyl-cytosinyl triphosphate + H2O
CDP + AMP
show the reaction diagram
-
-
-
-
?
adenosyl-cytosinyl triphosphate + H2O
CDP + AMP
show the reaction diagram
-
-
-
-
?
adenosyl-guanosine triphosphate + H2O
GDP + AMP
show the reaction diagram
-
-
-
-
?
adenosyl-guanosine triphosphate + H2O
GDP + AMP
show the reaction diagram
-
-
-
-
?
adenosyl-guanosine triphosphate + H2O
GDP + AMP
show the reaction diagram
-
-
-
-
?
adenosyl-ribose triphosphate + H2O
AMP + ribose diphosphate
show the reaction diagram
-
-
-
-
?
adenylylfluoride + H2O
AMP + fluoride
show the reaction diagram
Q9LVM4
-
-
-
?
Ap3A + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
Ap4C + H2O
AMP + CTP
show the reaction diagram
-
-
-
-
?
Ap4G + H2O
AMP + GTP + ATP + GMP
show the reaction diagram
-
-
-
-
?
Ap4U + H2O
AMP + UTP
show the reaction diagram
-
-
-
-
?
Ap6A + H2O
?
show the reaction diagram
-
-
-
-
?
ApCF2ppA
?
show the reaction diagram
-
-
-
-
?
ApCH2ppA
?
show the reaction diagram
-
-
-
-
?
ApCHFppA
?
show the reaction diagram
-
-
-
-
?
Appp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
AMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
show the reaction diagram
-
-
-
-
?
Appp-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin + H2O
AMP + diphospho-S-(7-diethylamino-4-methyl-3-(4-succinimidylphenyl)) coumarin
show the reaction diagram
-
-
-
-
?
ATP + 2 H2O
AMP + 2 phosphate
show the reaction diagram
-
-
-
-
?
cytosine 5'-phosphoimidazolide + UDP
imidazole + C(5')p3(5')U
show the reaction diagram
-
H96G mutant
-
-
?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
show the reaction diagram
-
1% initial velocity of degradation
-
-
?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
show the reaction diagram
-
100% initial velocity of degradation
-
-
?
di(7-methylguanosine) 50,50 0 0-P1,P3-triphosphate + H2O
?
show the reaction diagram
-
8% initial velocity of degradation
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
AMP + ATP
show the reaction diagram
-
-
-
-
-
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
AMP + ATP
show the reaction diagram
-
-
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
AMP + ATP
show the reaction diagram
-
-
-
-
?
diadenosine 5',5''-P1,P4-tetraphosphate + H2O
AMP + ATP
show the reaction diagram
-
-
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
show the reaction diagram
-
-
-
-
-
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
show the reaction diagram
-
-
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
show the reaction diagram
-
-
-
-
?
diadenosine 5',5''-P1,P5-pentaphosphate + H2O
AMP + adenosine 5'-tetraphosphate
show the reaction diagram
-
-
-
-
?
Dixanthosine triphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
gamma-fluoro-ATP + H2O
AMP + fluorodiphosphoric acid
show the reaction diagram
Q9LVM4
-
-
-
?
Gp3C + H2O
GMP + CDP
show the reaction diagram
-
-
-
-
?
Gp4A + H2O
?
show the reaction diagram
-
-
-
-
?
Gp4G + H2O
GMP + GTP
show the reaction diagram
-
-
-
-
?
Gp5A + H2O
?
show the reaction diagram
-
-
-
-
?
Gppp-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl + H2O
GMP + diphospho-S-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacine-3-yl) methylaminoacetyl
show the reaction diagram
-
-
-
-
?
GpppA + H2O
guanosine monophosphate + ppAppG + adenosine monophosphate
show the reaction diagram
Trypanosoma brucei, Trypanosoma brucei 427
-
-
-
-
?
inosine 5'-phosphoramidate + H2O
IMP + NH3
show the reaction diagram
-
-
-
-
?
inosine 5'-phosphoramidate + H2O
IMP + NH3
show the reaction diagram
Q9LVM4
-
-
-
?
m27,2'OGpppG + H2O
ppm27,2'OG + guanosine monophosphate
show the reaction diagram
Trypanosoma brucei, Trypanosoma brucei 427
-
-
-
-
?
m2N2,7GpppG + H2O
pm2N2,7G + guanosine diphosphate
show the reaction diagram
-
-
-
-
?
m3N2,N2,7GpppA + H2O
ppm3N2,N2,7G + adenosine monophosphate
show the reaction diagram
-
-
-
-
?
m3N2,N2,7GpppG + H2O
ppm3N2,N2,7G + guanosine monophosphate
show the reaction diagram
-
-
-
-
?
m7GpppA + H2O
pm7G + ppA ppm7G + adenosine monophosphate
show the reaction diagram
-
-
-
-
?
m7GpppG + H2O
pm7G + guanosine diphosphate
show the reaction diagram
-
-
-
-
?
m7Gpppm3N6,N6,29OA + H2O
pm7G + ppm3N6,N6,29OA
show the reaction diagram
-
-
-
-
?
m7Gpppm7G + H2O
pm7G+ppm7G
show the reaction diagram
-
-
-
-
?
NAD+ + H2O
?
show the reaction diagram
-
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
Q9LVM4
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
FHIT protein has been shown to function as a dinucleoside 5',5'''-P1,P3-triphosphate hydrolase, forming ADP and AMP from the Ap3A substrate. Tumor suppression by FHIT occurs in lung, gastric, and renal carcinoma-derived cell lines, with Ap3A hydrolysis by FHIT being not necessary for the tumor suppressive activity
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
Q9LVM4
requirement for Mg2+
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
27% initial velocity of degradation
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
58% initial velocity of degradation
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
show the reaction diagram
-
72% initial velocity of degradation
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
AMP + ADP
show the reaction diagram
-
major substrate
-
-
?
P1,P3-bis(5-(7-methylguanosyl))triphosphate + H2O
?
show the reaction diagram
-
1% initial velocities of degradation
-
-
?
P1,P3-bis(5-(7-methylguanosyl))triphosphate + H2O
?
show the reaction diagram
-
100% initial velocities of degradation
-
-
?
P1,P3-bis(5-(7-methylguanosyl))triphosphate + H2O
?
show the reaction diagram
-
8% initial velocities of degradation
-
-
?
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
AMP + ATP
show the reaction diagram
-
major substrate
-
-
?
P1,P4-bis(5-adenosyl)tetraphosphate + H2O
ADP + AMP
show the reaction diagram
-
27% initial velocities of degradation
-
-
?
P1,P4-bis(5-adenosyl)tetraphosphate + H2O
ADP + AMP
show the reaction diagram
-
58% initial velocities of degradation
-
-
?
P1,P4-bis(5-adenosyl)tetraphosphate + H2O
ADP + AMP
show the reaction diagram
-
72% initial velocities of degradation
-
-
?
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate + H2O
AMP + ADP
show the reaction diagram
-
-
-
-
r
P1-bis(5'-adenosyl) monophosphate + H2O
?
show the reaction diagram
Q9LVM4
-, requirement for Mg2+
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
P49789
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
hydrolysis of Ap3A
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
Trypanosoma brucei, Trypanosoma brucei 427
-
highest specificity
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
show the reaction diagram
-
-
-
-
-
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-guanosyl) triphosphate + H2O
GDP + GMP
show the reaction diagram
Trypanosoma brucei 427
-
-
-
-
?
P1-P3-bis(5'-uracyl) triphosphate + H2O
UDP + UMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-uracyl) triphosphate + H2O
UDP + UMP
show the reaction diagram
-
-
-
-
?
P1-P5-bis(5'-adenosyl) pentaphosphate + H2O
adenosine monophosphate + adenosine tetraphosphate
show the reaction diagram
-
-
-
-
?
thymidine 5'-monophosphate p-nitrophenylester + H2O
?
show the reaction diagram
-
-
-
-
?
thymidine 5'-phosphoimidazolide + UDP
imidazole + T(5')p3(5')U
show the reaction diagram
-
H96G mutant
-
-
?
Up3G + H2O
GMP + UDP
show the reaction diagram
-
-
-
-
?
UTP + H2O
UMP + phosphate
show the reaction diagram
-
-
-
-
?
m7GpppmN6A + H2O
pm7G + ppmN6A
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
Fhit is a tumor suppressor protein
-
-
-
additional information
?
-
-
Fhit protein inhibits cell growth by attenuating the signaling mediated by nuclear factor-kappaB in colon cancer cell lines
-
-
-
additional information
?
-
-
diadenosine oligophosphates, ApnA, have emerged as putative extra- and intracellular signaling molecules implicated in the maintenance and regulation of vital cellular functions and are now considered as a new class of signal transducers. FHIT induction protects from tumor necrosis factor-related apoptosis inducing ligand-induced cell death downstream TRAIL-receptors and likely requires its dinucleoside-triphosphate hydrolase activity, overview
-
-
-
additional information
?
-
Q9LVM4
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
-
additional information
?
-
-
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 50-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview, the dinucleoside triphosphatase preferentially hydrolyzes NpppN' to an NMP and N'DP
-
-
-
additional information
?
-
Q9LVM4
the dinucleoside triphosphatase preferentially hydrolyzes NpppN' to an NMP and N'DP
-
-
-
additional information
?
-
Trypanosoma brucei, Trypanosoma brucei 427
-
the enzyme does not hydrolyze Cap 4 m7Gpppm3N6,N6,2'O, Apm2'OApm2'OCpm2N3,2'OU, m7GpppApApCpU, m7GpCH2ppG, and m7Gpppm3N6,N6,2'OApm29OA
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenosine 5'-phosphoramidate + H2O
AMP + NH3
show the reaction diagram
-
-
-
-
?
adenosine 5'-phosphoramidate + H2O
AMP + NH3
show the reaction diagram
Q9LVM4
no requirement for Mg2+
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
show the reaction diagram
Q9LVM4
no requirement for Mg2+, reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenosine 5'-phosphosulfate + H2O
AMP + sulfate
show the reaction diagram
-
reaction of EC 3.6.2.1, adenylylsulfatase
-
-
?
adenylylfluoride + H2O
AMP + fluoride
show the reaction diagram
Q9LVM4
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
FHIT protein has been shown to function as a dinucleoside 5',5'''-P1,P3-triphosphate hydrolase, forming ADP and AMP from the Ap3A substrate. Tumor suppression by FHIT occurs in lung, gastric, and renal carcinoma-derived cell lines, with Ap3A hydrolysis by FHIT being not necessary for the tumor suppressive activity
-
-
?
P1,P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
Q9LVM4
requirement for Mg2+
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1,P3-bis(5'-adenosyl)triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate + H2O
AMP + ADP
show the reaction diagram
-
-
-
-
r
P1-bis(5'-adenosyl) monophosphate + H2O
?
show the reaction diagram
Q9LVM4
requirement for Mg2+
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
P49789
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
P1-P3-bis(5'-adenosyl) triphosphate + H2O
ADP + AMP
show the reaction diagram
-
-
-
-
?
gamma-fluoro-ATP + H2O
AMP + fluorodiphosphoric acid
show the reaction diagram
Q9LVM4
-
-
-
?
additional information
?
-
-
Fhit is a tumor suppressor protein
-
-
-
additional information
?
-
-
Fhit protein inhibits cell growth by attenuating the signaling mediated by nuclear factor-kappaB in colon cancer cell lines
-
-
-
additional information
?
-
-
diadenosine oligophosphates, ApnA, have emerged as putative extra- and intracellular signaling molecules implicated in the maintenance and regulation of vital cellular functions and are now considered as a new class of signal transducers. FHIT induction protects from tumor necrosis factor-related apoptosis inducing ligand-induced cell death downstream TRAIL-receptors and likely requires its dinucleoside-triphosphate hydrolase activity, overview
-
-
-
additional information
?
-
Q9LVM4
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 5'-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
-
additional information
?
-
-
Fhit proteins, in addition to their function as dinucleoside triphosphate hydrolases, act similarly to adenylylsulfatases and nucleoside phosphoramidases, liberating nucleoside 5'-monophosphates from such natural metabolites as adenosine 5'-phosphosulfate and adenosine 5'-phosphoramidate. Moreover, Fhits recognize synthetic nucleotides, such as adenosine 50-O-phosphorofluoridate and adenosine 5'-O-(gamma-fluorotriphosphate), and release AMP from them, the enzyme is involved in controlling the cellular level of NpnN0', overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
-
Ca2+
-
required for activity
Ca2+
-
-
Ca2+
-
maximal activation at 0.5-1.0 mM
Co2+
-
-
Mg2+
-
required for activity
Mg2+
-
required for activity
Mg2+
-
Mg2+-dependent
Mg2+
-
-
Mg2+
-
required for the synthesis of dinucleoside tri- and tetraphosphates by H96G mutant
Mg2+
-
dependent on, formation of an MgADP- intermediate during catalytic reaction
Mg2+
-
dependent on
Mg2+
-
dependent on, maximal activation at 0.5-1.0 mM
Mn2+
-
required for activity
Mn2+
-
-
Mn2+
-
-
Mn2+
-
activates
Zn2+
-
-
Mn2+
-
maximal activation at 0.5-1.0 mM
additional information
-
no requirement for Mg2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
adenosine 5'-O-(gamma-fluoromonophosphate)
Q9LVM4
-
adenosine 5'-O-(gamma-fluorotriphosphate)
Q9LVM4
-
adenosine 5'-phosphosulfate
Q9LVM4
substrate inhibition
adenosine 5'-phosphosulfate
-
substrate inhibition
ADP
-
competitive inhibitor
AMP
-
competitive inhibitor
AMP
Q9LVM4
product inhibition
AMP
-
product inhibition
ATP
-
competitive inhibitor
diethyl dicarbonate
-
inactivation
O,O',O''-tris[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl] O,O',O''-propane-1,2,3-triyl tris[hydrogen (phosphorothioate)]
-
-
O,O'-bis[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl] O,O'-(2-hydroxypropane-1,3-diyl) bis[hydrogen (phosphorothioate)]
-
-
O-[1,11-bis[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]-3,9-dioxido-3,9-disulfanyl-2,4,8,10-tetraoxa-3l5,9l5-diphosphaundecan-6-yl] dihydrogen phosphorothioate
-
-
O-[3-[[[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy](sulfanyl)phosphoryl]oxy]-2,2-bis([[[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy](sulfanyl)phosphoryl]oxy]methyl)propyl] dihydrogen phosphorothioate
-
-
O-[3-[[[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy](sulfanyl)phosphoryl]oxy]-2,2-bis([[[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy](sulfanyl)phosphoryl]oxy]methyl)propyl] O-[[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl] hydrogen phosphorothioate
-
-
P1,P3-bis(5'-adenosyl) triphosphate
Q9LVM4
substrate inhibition
P1,P3-bis(5'-adenosyl) triphosphate
-
substrate inhibition
suramin
-
0.1 nM
suramin
-
strong, competitive inhibition, IC50: 0.002 mM
Zn2+
-
competitive inhibitor
Zn2+
-
IC50: 0.02 mM
additional information
-
poor inhibition by fluoroadenylates, ATP, and ADP
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.003
-
5'-O-[amino(hydroxy)phosphoryl]adenosine
Q9LVM4
pH 6.8, 30C
0.003
-
5'-O-[amino(hydroxy)phosphoryl]adenosine
-
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
0.015
-
5'-O-[amino(hydroxy)phosphoryl]adenosine
-
in 0.1 M potassium phosphate (pH 6.8), 5 mM MgCl2, at 30C
0.003
-
adenosine 5'-phosphoramidate
-
pH 6.8, 30C
0.0053
-
adenosine-5'-phospho-N-methylimidazolide
-
wild-type enzyme, pH 6.6
0.18
-
adenosine-5'-phospho-N-methylimidazolide
-
mutant H96G, pH 6.6
0.0062
-
adenosine-5'-phosphoimidazolide
-
wild-type enzyme, pH 5.8
0.026
-
adenosine-5'-phosphoimidazolide
-
mutant H96G, pH 5.8
0.0006
-
Ap4A
-
-
0.0026
-
Ap4A
-
-
0.0046
-
Ap4A
-
-
0.013
-
Ap5A
-
-
0.212
-
ApCF2ppA
-
-
0.117
-
ApCH2ppA
-
-
0.00066
-
P1,P3-bis(5'-adenosyl)triphosphate
-
diphosphorylated recombinant enzyme, pH 6.8, 37C
0.00067
-
P1,P3-bis(5'-adenosyl)triphosphate
-
monophosphorylated recombinant enzyme, pH 6.8, 37C
0.0014
-
P1,P3-bis(5'-adenosyl)triphosphate
-
pH 7.0, 37C
0.0016
-
P1,P3-bis(5'-adenosyl)triphosphate
-
nonphosphorylated recombinant enzyme, pH 6.8, 37C
0.0029
-
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
-
wild-type enzyme, pH 7.3
0.026
-
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
-
mutant H96G, pH 8.9
0.001
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.001
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.001
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.0013
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.0015
-
P1-P3-bis(5'-adenosyl) triphosphate
-
Ap3Aase-alpha and -c
0.0019
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.0035
-
P1-P3-bis(5'-adenosyl) triphosphate
-
Ap3Aase-beta
0.0054
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.007
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.012
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.012
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.014
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.015
-
P1-P3-bis(5'-adenosyl) triphosphate
-
-
0.002
-
P1-P3-bis(5'-guanosyl) triphosphate
-
-
0.01
-
P1-P3-bis(5'-guanosyl) triphosphate
-
-
0.04
-
P1-P3-bis(5'-guanosyl) triphosphate
-
-
0.025
-
P1-P3-bis(5'-uracyl) triphosphate
-
-
20
-
UDP
-
pH 7, synthesis of Ap3U by H96G mutant
0.09
-
ApCHFppA
-
-
additional information
-
additional information
-
steady state kinetics of the phosphorylated enzyme form
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.26
-
5'-O-[amino(hydroxy)phosphoryl]adenosine
Q9LVM4
pH 6.8, 30C
1.26
-
adenosine 5'-phosphoramidate
-
pH 6.8, 30C
0.62
-
adenosine-5'-phospho-N-methylimidazolide
-
mutant H96G, pH 6.6
4.2
-
adenosine-5'-phospho-N-methylimidazolide
-
wild-type enzyme, pH 6.6
0.91
-
adenosine-5'-phosphoimidazolide
-
mutant H96G, pH 5.8
4.4
-
adenosine-5'-phosphoimidazolide
-
wild-type enzyme, pH 5.8
0.02
-
P1,P3-bis(5'-adenosyl)triphosphate
-
diphosphorylated recombinant enzyme, pH 6.8, 37C
0.38
-
P1,P3-bis(5'-adenosyl)triphosphate
-
monophosphorylated recombinant enzyme, pH 6.8, 37C
2.74
-
P1,P3-bis(5'-adenosyl)triphosphate
-
nonphosphorylated recombinant enzyme, pH 6.8, 37C
3
6
P1,P3-bis(5'-adenosyl)triphosphate
-
nonphosphorylated recombinant enzyme, pH 6.8, 37C
0.0014
-
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
-
mutant H96G, pH 8.9
3.5
-
P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
-
wild-type enzyme, pH 7.3
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.018
-
adenosine 5'-O-(gamma-fluoromonophosphate)
Q9LVM4
pH 6.8, 30C
0.235
-
adenosine 5'-O-(gamma-fluoromonophosphate)
Q9LVM4
pH 6.8, 30C, in presence of Mg2+
0.0071
-
adenosine 5'-phosphosulfate
Q9LVM4
pH 6.8, 30C
0.0136
-
adenosine 5'-phosphosulfate
-
pH 6.8, 30C
0.14
-
ADP
Q9LVM4
pH 6.8, 30C
0.11
-
AMP
-
pH 6.8, 30C
0.113
-
AMP
Q9LVM4
pH 6.8, 30C
0.145
-
ATP
Q9LVM4
pH 6.8, 30C
0.0021
-
P1,P3-bis(5'-adenosyl) triphosphate
Q9LVM4
pH 6.8, 30C, in presence of Mg2+
0.018
-
P1,P3-bis(5'-adenosyl) triphosphate
-
pH 6.8, 30C, in presence of Mg2+
0.000022
-
suramin
-
pH 7.0, 37C
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.002
-
suramin
-
strong, competitive inhibition, IC50: 0.002 mM
0.02
-
Zn2+
-
IC50: 0.02 mM
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0075
-
-
-
0.02
-
-
-
0.07
-
-
-
0.4
-
-
skeletal muscle, 27000 x g supernatant
1.34
-
-
purified native enzyme from leukocytes
1.8
-
-
brain, 27000 x g supernatant
4.8
-
-
liver, 27000 x g precipitate
13
-
-
kidney, 27000 x g precipitate
290
-
-
Ap3Aase-alpha
300
-
-
Ap3Aase-beta
additional information
-
-
-
additional information
-
Q9LVM4
relative velocities of hydrolysis with different substrates, overview
additional information
-
-
relative velocities of hydrolysis with different substrates, overview
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.8
-
-
optimal pH for hydrolysis of adenosine-5'-phosphoimidazolide
6.6
-
-
optimal pH for hydrolysis of adenosine-5'-phospho-N-methylimidazolide
6.8
-
-
assay at
6.8
-
Q9LVM4
assay at
6.8
-
-
assay at
7
7.2
-
-
7.3
-
-
optimal pH for hydrolysis of P1-5'-O-adenosine-P3-5'-O-adenosine triphosphate
8.2
8.5
-
-
8.5
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.6
10
-
-
6.6
9.5
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
-
30
-
Q9LVM4
assay at
30
-
-
assay at
37
-
-
assay at
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
45
-
-
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
a human SV40-immortalized normal bronchial epithelial cell line
Manually annotated by BRENDA team
-
colonic cancer cell line
Manually annotated by BRENDA team
-
colonic cancer cell line
Manually annotated by BRENDA team
-
colonic cancer cell line
Manually annotated by BRENDA team
-
FHIT expression level and distribution in neoplastic, hyperplastic, and healthy endometrium, overview
Manually annotated by BRENDA team
-
of secretory endometrium
Manually annotated by BRENDA team
-
of secretory endometrium
Manually annotated by BRENDA team
-
colonic cancer cell line
Manually annotated by BRENDA team
additional information
-
FHIT expression analysis of wild-type and mutant enzyme in colonic cancer cell lines, overview
Manually annotated by BRENDA team
additional information
-
detailed FHIT expression analysis in human tissues, the expression is reduced about 30-100% in cancer cells compared to healthy cells in diverse tissue types, no expression in lymphoma cells, overview
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
29800
-
-
gel filtration
34000
-
-
gel filtration
36000
-
-
gel filtration
36000
-
-
gel filtration
41000
-
-
-
41000
-
-
gel filtration, Ap3A hydrolase I
55000
-
-
gel filtration
115000
-
-
gel filtration
230000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
-
dimer
-
2 * 17000
homodimer
-
alpha2, 2 * 20500, SDS-PAGE
homodimer
-
-
monomer
-
alpha, 1 * 36000, SDS-PAGE
monomer
-
alpha 1 * 41000, SDS-PAGE, Ap3A hydrolase I
polymer
-
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
mannoside
phosphoprotein
-
Fhit is phosphorylated in vivo, and is phosphorylated in vitro by Src kinase
phosphoprotein
-
FHIT is phosphorylated at Tyr114
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, 50 mM HEPES-NaOH buffer, pH 7.0, 100 mM NaCl, 25% glycerol, at least 18 months
-
4C, 50 mM HEPES-NaOH buffer, pH 7.0, 100 mM NaCl, 3 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partially
-
co-expression of Fhit with the elk tyrosine kinase in Escherichia coli strain TBK1 by ion exchange chromatography, gel filtration, and another step of ion exchange chromatography
-
native enzyme from blood platelets and leukocytes, 19000fold, by anion exchange chromatography and N6-5'-AMP affinity chromatography to homogeneity
-
native enzyme from brain cytosolic fraction by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and N6-5'-AMP affinity chromatography to homogeneity
-
Ni-NTA agarose column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gene At5g58240, DNA and amino acid sequence determination and analysis
Q9LVM4
gene FHIT, DNA sequence determination and analysis, comparative mapping study, the gene locates to BTA22q24prox
Q4KTA6
co-expression of Fhit with the elk tyrosine kinase in Escherichia coli strain TBK1, expression of nonphosphorylized enzyme in Escherichia coli strain SG100
-
constitutive expression of FHIT in Calu-1 cells, endogenously lacking FHIT protein, FHIT re-expression does not affect cell survival, but a Calu-1 cell clone in which the expression of FHIT is under the control of a CMV promoter, is more resistant to TRAIL-induced, i.e. tumor necrosis factor-related apoptosis inducing ligand-induced, apoptosis than the mock transfected Calu-1 clones
-
expression in COS1 cells; expression in Escherichia coli
-
expression in Escherichia coli
-
FHIT expression analysis of wild-type and mutant enzyme in colon cancer cell lines, overview
-
gene fhit maps to chromosome 3q14.2, expression analysis in human tissues, overview
-
expressed in Escherichia coli BL21(DE3) cells
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
FHIT is absent or reduced in many types of human tumors including lung, esophagus, stomach, kidney and cervical carcinomas
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ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
H96G
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mutant Fhit can not hydrolyse phosphoanhydrides, but can synthetise dinucleoside tri- and tetraphosphates
H355N
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inactive
H355N
Trypanosoma brucei 427
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inactive
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H96G
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site-directed mutagenesis in the fragile histidine triad, leads to reduced activity and reduced hydrolysis of reaction intermediates
additional information
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FHIT-specific small interference RNA transfection of SV40-immortalized normal bronchial BEAS cells that show levels of FHIT protein comparable to those of normal bronchial cells, results in a significant increase of TRAIL-induced apoptosis