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Information on EC 3.6.1.27 - undecaprenyl-diphosphate phosphatase and Organism(s) Micrococcus luteus and UniProt Accession C5CBT8

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IUBMB Comments
Isolated from the bacteria Micrococcus lysodeikticus , Escherichia coli [2,3,5,6] and Bacillus subtilis . The product of the reaction, ditrans,octacis-undecaprenyl phosphate, is essential for cell wall polysaccharide biosynthesis in these strains.
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Micrococcus luteus
UNIPROT: C5CBT8
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Word Map
The taxonomic range for the selected organisms is: Micrococcus luteus
The enzyme appears in selected viruses and cellular organisms
Synonyms
undecaprenyl pyrophosphate phosphatase, upp phosphatase, smu.244, c55-pp phosphatase, undecaprenyl diphosphate phosphatase, c55-pp pyrophosphatase, undecaprenyl pyrophosphatase, hp0851, undecaprenyl-pyrophosphate phosphatase, dolicholpyrophosphatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C55-PP phosphatase
-
undecaprenyl pyrophosphate phosphatase
-
C55-isoprenyl diphosphatase
-
-
-
-
C55-isoprenyl pyrophosphatase
-
-
-
-
Dolicholpyrophosphatase
-
-
-
-
isoprenyl pyrophosphatase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
ditrans,octacis-undecaprenyl-diphosphate phosphohydrolase
Isolated from the bacteria Micrococcus lysodeikticus [1], Escherichia coli [2,3,5,6] and Bacillus subtilis [4]. The product of the reaction, ditrans,octacis-undecaprenyl phosphate, is essential for cell wall polysaccharide biosynthesis in these strains.
CAS REGISTRY NUMBER
COMMENTARY hide
9077-80-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
undecaprenyl diphosphate + H2O
undecaprenyl phosphate + phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
undecaprenyl diphosphate + H2O
undecaprenyl phosphate + phosphate
show the reaction diagram
-
involved in cyclic peptidoglycan pathway
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no requirement for added metal ions
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
tripropeptin C
tripropeptin C can potentially inhibit C55-PP phosphatase activity, which plays a crucial role in the lipid cycle of peptidoglycan synthesis
DTNB
-
slight inhibition
n-butanol
-
-
n-Propanol
-
inhibitory at concentrations above 1.5 M
p-chloromercuriphenylsulfonic acid
-
-
tetrahydrofuran
-
inhibitory at concentrations above 2 M
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethanol
-
slight activation at concentration of 4 M
methanol
-
slight activation at concentration of 4 M
n-Propanol
-
activating at concentrations below 1.5 M
Triton X-100
-
-
Triton X-114
-
-
additional information
-
significant rise in activity at high ionic strength
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
undecaprenyl diphosphate
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00003 - 0.0001
tripropeptin C
Micrococcus luteus
in 100 mM Tris-HCl, 10 mM MgCl2 and 3.9 mM n-dodecyl-beta-maltoside (pH 7.5), 1.25 mM CaCl2, at 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3 - 7.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-20
-
significant loss of activity by freezing and thawing one time
25
-
solubilized enzyme stable for 30 min below 25°C, loss of activity with higher temperatures
4
-
30-40% loss of activity within 1 week
40
-
particular enzyme stable for 30 min below 40°C, loss of activity with higher temperatures
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ascorbic acid stabilizes
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Goldman, R.; Strominger, J.L.
Purification and properties of C 55 -isoprenylpyrophosphate phosphatase from Micrococcus lysodeikticus
J. Biol. Chem.
247
5116-5122
1972
Micrococcus luteus
Manually annotated by BRENDA team
Hashizume, H.; Sawa, R.; Harada, S.; Igarashi, M.; Adachi, H.; Nishimura, Y.; Nomoto, A.
Tripropeptin C blocks the lipid cycle of cell wall biosynthesis by complex formation with undecaprenyl pyrophosphate
Antimicrob. Agents Chemother.
55
3821-3828
2011
Micrococcus luteus (C5CBT8)
Manually annotated by BRENDA team