Information on EC 3.6.1.24 - nucleoside phosphoacylhydrolase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
3.6.1.24
-
RECOMMENDED NAME
GeneOntology No.
nucleoside phosphoacylhydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolyses mixed phospho-anhydride bonds
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
nucleoside-5'-phosphoacylate acylhydrolase
Attacks ribonucleoside 5'-nitrophenylphosphates, but is inactive against phosphodiesters.
CAS REGISTRY NUMBER
COMMENTARY hide
37289-35-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl adenylate + H2O
?
show the reaction diagram
-
-
-
-
?
adenosine 5'-p-nitrophenylphosphate + H2O
p-nitrophenol + AMP
show the reaction diagram
-
-
-
?
cytidine 5'-p-nitrophenylphosphate + H2O
p-nitrophenol + CMP
show the reaction diagram
-
-
-
?
guanosine 5'-p-nitrophenylphosphate + H2O
p-nitrophenol + GMP
show the reaction diagram
-
-
-
?
methionyl adenylate + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl 5'-nucleotides + H2O
p-nitrophenol + nucleoside phosphate
show the reaction diagram
-
hydrolysis A,C,G,U,T nucleotides
-
?
thymidine 5'-p-nitrophenylphosphate + H2O
p-nitrophenol + TMP
show the reaction diagram
-
-
-
?
uridine 5'-p-nitrophenylphosphate + H2O
p-nitrophenol + UMP
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
p-nitrophenyl 5'-nucleotides + H2O
p-nitrophenol + nucleoside phosphate
show the reaction diagram
-
hydrolysis A,C,G,U,T nucleotides
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no metal ions required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alanyl adenylate
-
competitive inhibition of other substrates
aminoalkyl adenylate
-
strongest inhibitor, competitive inhibition of other substrates
AMP
-
competitive
KI
-
50% loss of activity at 1 M
lysyl adenylate
-
competitive inhibition of other substrates
Methionyl adenylate
-
competitive inhibition of other substrates
SDS
-
complete loss of activity at 6 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Li2SO4
-
160% of standard activity at 0.75 M
NH4Cl
-
180% of standard activity at 1 M
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.056
adenosine 5'-p-nitrophenylphosphate
-
-
4
cytidine 5'-p-nitrophenylphosphate
-
-
0.11
guanosine 5'-p-nitrophenylphosphate
-
-
770
thymidine 5'-p-nitrophenylphosphate
-
-
0.65
uridine 5'-p-nitrophenylphosphate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0018
-
crude extract
6670
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 10.5
-
very low activity at pH 5.5 and 10.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 65
-
rapid loss of activity above 50°C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
not periplasm
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23000
-
sedimentation equilibrium method, gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
irreversible inactivation below pH 7.5
209999
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
no loss of activity within one day
65
-
loss of activity above 65°C, can be restored by cooling down to 0°C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivation by repeated freezing and thawing
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
stable towards reducing agents
-
209999
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, can be stored for at least one year
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE