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IUBMB CommentsAttacks ribonucleoside 5'-nitrophenylphosphates, but is inactive against phosphodiesters.
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Hydrolyses mixed phospho-anhydride bonds
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Hydrolyses mixed phospho-anhydride bonds
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phosphorous acid anhydride hydrolysis
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nucleoside-5'-phosphoacylate acylhydrolase
Attacks ribonucleoside 5'-nitrophenylphosphates, but is inactive against phosphodiesters.
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acetyl adenylate + H2O
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adenosine 5'-p-nitrophenylphosphate + H2O
p-nitrophenol + AMP
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cytidine 5'-p-nitrophenylphosphate + H2O
p-nitrophenol + CMP
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guanosine 5'-p-nitrophenylphosphate + H2O
p-nitrophenol + GMP
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methionyl adenylate + H2O
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p-nitrophenyl 5'-nucleotides + H2O
p-nitrophenol + nucleoside phosphate
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hydrolysis A,C,G,U,T nucleotides
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thymidine 5'-p-nitrophenylphosphate + H2O
p-nitrophenol + TMP
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uridine 5'-p-nitrophenylphosphate + H2O
p-nitrophenol + UMP
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p-nitrophenyl 5'-nucleotides + H2O
p-nitrophenol + nucleoside phosphate
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hydrolysis A,C,G,U,T nucleotides
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additional information
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no metal ions required
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alanyl adenylate
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competitive inhibition of other substrates
aminoalkyl adenylate
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strongest inhibitor, competitive inhibition of other substrates
KI
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50% loss of activity at 1 M
lysyl adenylate
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competitive inhibition of other substrates
Methionyl adenylate
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competitive inhibition of other substrates
SDS
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complete loss of activity at 6 mM
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Li2SO4
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160% of standard activity at 0.75 M
NH4Cl
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180% of standard activity at 1 M
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0.056
adenosine 5'-p-nitrophenylphosphate
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4
cytidine 5'-p-nitrophenylphosphate
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0.11
guanosine 5'-p-nitrophenylphosphate
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770
thymidine 5'-p-nitrophenylphosphate
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0.65
uridine 5'-p-nitrophenylphosphate
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5.5 - 10.5
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very low activity at pH 5.5 and 10.5
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10 - 65
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rapid loss of activity above 50°C
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brenda
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additional information
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not periplasm
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brenda
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A9GN44_SORC5
Sorangium cellulosum (strain So ce56)
1011
0
107364
TrEMBL
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23000
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sedimentation equilibrium method, gel filtration
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7.5
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irreversible inactivation below pH 7.5
209999
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0
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no loss of activity within one day
65
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loss of activity above 65°C, can be restored by cooling down to 0°C
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inactivation by repeated freezing and thawing
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stable towards reducing agents
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209999
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-20°C, can be stored for at least one year
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Spahr, P.F.; Gesteland, R.F.
Purification and properties of a new enzyme from Escherichia coli: nucleoside phosphacyl hydrolase
Eur. J. Biochem.
12
270-284
1970
Escherichia coli
brenda
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