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Information on EC 3.6.1.15 - nucleoside-triphosphate phosphatase and Organism(s) Southampton virus and UniProt Accession Q04544

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IUBMB Comments
The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. Also hydrolyses nucleoside diphosphates, thiamine diphosphate and FAD. The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin .
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This record set is specific for:
Southampton virus
UNIPROT: Q04544
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Word Map
The taxonomic range for the selected organisms is: Southampton virus
The enzyme appears in selected viruses and cellular organisms
Synonyms
ntpase, ntpdase, nucleoside triphosphatase, ntpdase1, ntpdase2, nsp13, kidins220, nucleoside triphosphate diphosphohydrolase, ntpase/helicase, nucleoside triphosphate hydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleic acid-independent nucleoside triphosphatase
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polypeptide p41
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GP086L protein
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M86L protein
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-
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MC100R
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-
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NTPase
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-
-
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nucleoside 5-triphosphatase
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-
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nucleoside triphosphate phosphohydrolase
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-
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nucleoside-5-triphosphate phosphohydrolase
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phosphatase, nucleoside tri-
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
unspecific diphosphate phosphohydrolase
The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. Also hydrolyses nucleoside diphosphates, thiamine diphosphate and FAD. The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin [5].
CAS REGISTRY NUMBER
COMMENTARY hide
9075-51-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O
ADP + phosphate
show the reaction diagram
-
-
-
?
CTP + H2O
CDP + phosphate
show the reaction diagram
about 35% of activity with ATP
-
-
?
GTP + H2O
GDP + phosphate
show the reaction diagram
about 90% of activity with ATP
-
-
?
UTP + H2O
UDP + phosphate
show the reaction diagram
about 35% of activity with ATP
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-
?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
20 mM, about 50% of the maximal activation obtained with Mn2+
Mn2+
activates, 3.5-5 mM MnCl2 are optimal at an ATP concentration of 0.5 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
in presence of Mn2+, 10 mM MgCl2 inhibits 20%
MgCl2
in presence of Mn2+, 10 mM MgCl2 inhibits 10%
NaCl
100 mM, inhibits ATP hydrolysis by 30%
polyA
inhibits to a lesser extent than polyC and polyU
polyC
1 mg/l, 70% inhibition
polyG
inhibits to a lesser extent than polyC and polyU
polyU
1 mg/l, 70% inhibition
RNA
homopolymeric RNA inhibits under otherwise optimal conditions
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 7.5
ATPase activity
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 8
pH 6.8: about 90% of maximal activity, pH 8.0: about 70% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pfister, T.; Wimmer, E.
Polypeptide p41 of a Norwalk-like virus is a nucleic acid-independent nucleoside triphosphatase
J. Virol.
75
1611-1619
2001
Southampton virus (Q04544), Southampton virus
Manually annotated by BRENDA team