Information on EC 3.6.1.14 - adenosine-tetraphosphatase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
3.6.1.14
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RECOMMENDED NAME
GeneOntology No.
adenosine-tetraphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
adenosine 5'-tetraphosphate + H2O = ATP + phosphate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphorous acid anhydride hydrolysis
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Purine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
adenosine-tetraphosphate phosphohydrolase
Also acts on inosine tetraphosphate and tripolyphosphate but shows little or no activity with other nucleotides or polyphosphates.
CAS REGISTRY NUMBER
COMMENTARY hide
37289-26-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenosine 5'-pentaphosphate + H2O
?
show the reaction diagram
adenosine 5'-tetraphosphate + H2O
ATP + phosphate
show the reaction diagram
alpha-beta-methylene-adenosine 5'-tetraphosphate + H2O
alpha-beta-methylene-adenosine 5'-triphosphate + phosphate
show the reaction diagram
guanosine tetraphosphate + H2O
GTP + phosphate
show the reaction diagram
inosine tetraphosphate + H2O
ITP + phosphate
show the reaction diagram
tripolyphosphate + H2O
diphosphate + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
divalent metal ion required, the order of decreasing effectiveness for hydrolysis of adenosine 5'-tetraphosphate is: Co2+, Fe2+, Mg2+, Ni2+, Mn2+. For tripolyphosphate hydrolysis the order is: Ni2+, Co2+, Fe2+, Mg2+, Mn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ammonium molybdate
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N-ethylmaleimide
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p-hydroxymercuribenzoate
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sodium o-vanadate
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tripolyphosphate
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competitive inhibitor of adenosine tetraphosphate
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0027 - 0.08
adenosine 5'-tetraphosphate
0.0034
inosine tetraphosphate
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-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
723
adenosine 5'-tetraphosphate
Saccharomyces cerevisiae
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-
40
adenosine-5'-pentaphosphate
Saccharomyces cerevisiae
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.8 - 7.2
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50% of maximal activity at pH 3.8 and 7.2
7.6 - 9.4
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50% of maximal activity at pH 7.6 and 9.4
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 45000, SDS-PAGE
monomer
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1 * 25000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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enzyme is stable at the extremes of pH for at least 30 min
209873
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 6 months, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE