Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.6.1.12 - dCTP diphosphatase and Organism(s) Mus musculus and UniProt Accession Q9QY93

for references in articles please use BRENDA:EC3.6.1.12
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.12 dCTP diphosphatase
IUBMB Comments
The mammalian enzyme also displays weak activity against dTTP and dATP, but none against dGTP. Activity is highest with analogs including 5-iodo-dCTP and 5-methyl-dCTP.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Mus musculus
UNIPROT: Q9QY93
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
pyrophosphohydrolase, dctpp1, dctpase, rs21-c6, ntp pyrophosphohydrolase, dctp pyrophosphatase 1, dctpase-dutpase, ntp pyrophosphatase, dctp pyrophosphatase, xtp3-transactivated protein a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dCTP pyrophosphatase 1
-
dCTPase 1
-
dCTP pyrophosphatase
-
-
-
-
dCTPase
-
-
-
-
deoxy-CTPase
-
-
-
-
deoxycytidine triphosphatase
-
-
-
-
deoxycytidine-triphosphatase
-
-
-
-
phosphatase, deoxycytidine, tri-
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
dCTP nucleotidohydrolase
The mammalian enzyme also displays weak activity against dTTP and dATP, but none against dGTP. Activity is highest with analogs including 5-iodo-dCTP and 5-methyl-dCTP.
CAS REGISTRY NUMBER
COMMENTARY hide
9024-87-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-chloro-dATP + H2O
2-chloro-AMP + diphosphate
show the reaction diagram
-
-
-
?
2-hydroxy-dATP + H2O
2-hydroxy-dAMP + diphosphate
show the reaction diagram
-
-
-
?
5-bromo-dCTP + H2O
5-bromo-dCMP + diphosphate
show the reaction diagram
-
-
-
?
5-chloro-dCTP + H2O
5-chloro-dCMP + diphosphate
show the reaction diagram
-
-
-
?
5-fluoro-dUTP + H2O
5-fluoro-dUMP + diphosphate
show the reaction diagram
-
-
-
?
5-hydroxy-dCTP + H2O
5-hydroxy-dCMP + diphosphate
show the reaction diagram
-
-
-
?
5-iodo-dCTP + H2O
5-iodo-dCMP + diphosphate
show the reaction diagram
-
-
-
?
5-methyl-dCTP + H2O
5-methyl-dCMP + diphosphate
show the reaction diagram
-
-
-
?
CTP + H2O
CMP + diphosphate
show the reaction diagram
-
-
-
?
dATP + H2O
dAMP + diphosphate
show the reaction diagram
-
-
-
?
dCTP + H2O
dCMP + diphosphate
show the reaction diagram
-
-
-
?
dTTP + H2O
dTMP + diphosphate
show the reaction diagram
-
-
-
?
dUTP + H2O
dUMP + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MgCl2
maximum activity in presence of 100 mM MgCl2
MnCl2
up to 40% of the activity with MgCl2
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.107
2-chloro-dATP
pH 8.0, 30°C
0.169
2-hydroxy-dATP
pH 8.0, 30°C
0.0217
5-bromo-dCTP
pH 8.0, 30°C
0.05
5-chloro-dCTP
pH 8.0, 30°C
0.034
5-fluoro-dUTP
pH 8.0, 30°C
0.164
5-hydroxy-dCTP
pH 8.0, 30°C
0.0039
5-iodo-dCTP
pH 8.0, 30°C
0.0485
5-Methyl-dCTP
pH 8.0, 30°C
0.529
CTP
pH 8.0, 30°C
0.118
dATP
pH 8.0, 30°C
0.0441
dCTP
pH 8.0, 30°C
0.407
dTTP
pH 8.0, 30°C
0.484
dUTP
pH 8.0, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.57
2-chloro-dATP
pH 8.0, 30°C
1.39
2-hydroxy-dATP
pH 8.0, 30°C
1.49
5-bromo-dCTP
pH 8.0, 30°C
1.5
5-chloro-dCTP
pH 8.0, 30°C
0.58
5-fluoro-dUTP
pH 8.0, 30°C
0.87
5-hydroxy-dCTP
pH 8.0, 30°C
0.94
5-iodo-dCTP
pH 8.0, 30°C
1.33
5-Methyl-dCTP
pH 8.0, 30°C
0.31
CTP
pH 8.0, 30°C
0.53
dATP
pH 8.0, 30°C
1.37
dCTP
pH 8.0, 30°C
0.76
dTTP
pH 8.0, 30°C
0.76
dUTP
pH 8.0, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14.7
2-chloro-dATP
pH 8.0, 30°C
8.22
2-hydroxy-dATP
pH 8.0, 30°C
68.7
5-bromo-dCTP
pH 8.0, 30°C
30
5-chloro-dCTP
pH 8.0, 30°C
1.9
5-fluoro-dUTP
pH 8.0, 30°C
5.3
5-hydroxy-dCTP
pH 8.0, 30°C
241
5-iodo-dCTP
pH 8.0, 30°C
27.4
5-Methyl-dCTP
pH 8.0, 30°C
0.156
CTP
pH 8.0, 30°C
4.49
dATP
pH 8.0, 30°C
31.1
dCTP
pH 8.0, 30°C
1.87
dTTP
pH 8.0, 30°C
1.57
dUTP
pH 8.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
temperature-dependent increase of activity up to 60°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Manually annotated by BRENDA team
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Manually annotated by BRENDA team
enzyme is ubiquitously expressed. Expression is highest in the liver and heart, and to a lesser extent the salivary gland
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
exclusivelyx located in the cytosol
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DCTP1_MOUSE
170
0
18795
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18783
x * 19000, SDS-PAGE, x * 18783, calculated
19000
x * 19000, SDS-PAGE, x * 18783, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 19000, SDS-PAGE, x * 18783, calculated
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nonaka, M.; Tsuchimoto, D.; Sakumi, K.; Nakabeppu, Y.
Mouse RS21-C6 is a mammalian 2'-deoxycytidine 5'-triphosphate pyrophosphohydrolase that prefers 5-iodocytosine
FEBS J.
276
1654-1666
2009
Mus musculus (Q9QY93), Mus musculus
Manually annotated by BRENDA team