Information on EC 3.5.99.8 - 5-nitroanthranilic acid aminohydrolase

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The expected taxonomic range for this enzyme is: Bradyrhizobium

EC NUMBER
COMMENTARY hide
3.5.99.8
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RECOMMENDED NAME
GeneOntology No.
5-nitroanthranilic acid aminohydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-nitroanthranilate + H2O = 5-nitrosalicylate + NH3
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
5-nitroanthranilate degradation
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SYSTEMATIC NAME
IUBMB Comments
5-nitroanthranilate aminohydrolase
The enzyme catalyses the initial step in biodegradation of 5-nitroanthranilic acid by Bradyrhizobium sp. strain JS329.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-nitroanthranilate + H2O
5-nitrosalicylate + NH3
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-nitroanthranilate + H2O
5-nitrosalicylate + NH3
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
no added cofactors are required for the transformation
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
best stimulating divalent cation
additional information
NaaA contains the sequence HEAWH (amino acid residues 108 to 112), which is similar to the zinc binding motif HEXXH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
inhibits 70% at 1 mM
EDTA
inhibits 99% at 1 mM
o-phenanthroline
inhibits 98% at 1 mM
additional information
the activity of NaaA after inhibition is restored by the addition of divalent metal ions: e.g. Co2+ to 100%, Mn2+ to 85%, Zn2+ to 81%, Fe2+ to 67%, or Ni2+ to 39%, all at 0.667 mM
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain Rosetta 2/pJS803 by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene naaA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain Rosetta 2/pJS803 and in strain EPI300(pJS800)