Information on EC 3.5.99.10 - 2-iminobutanoate/2-iminopropanoate deaminase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.5.99.10
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RECOMMENDED NAME
GeneOntology No.
2-iminobutanoate/2-iminopropanoate deaminase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-iminobutanoate + H2O = 2-oxobutanoate + NH3
show the reaction diagram
2-iminopropanoate + H2O = pyruvate + NH3
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-serine degradation
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glycine betaine degradation I
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L-cysteine degradation II
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L-serine degradation
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L-tryptophan degradation II (via pyruvate)
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purine nucleobases degradation II (anaerobic)
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SYSTEMATIC NAME
IUBMB Comments
2-iminobutanoate aminohydrolase
This enzyme, which has been found in all species and tissues examined, catalyses the hydrolytic deamination of imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases, such as EC 4.3.1.19, threonine ammonia-lyase and EC 4.3.1.17, L-serine ammonia-lyase. The reactions, which can occur spontaneously, are accelerated to minimize the cellular damage that could be caused by these reactive intermediates.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-iminobutanoate + H2O
2-oxobutanoate + NH3
show the reaction diagram
2-iminopropanoate + H2O
pyruvate + NH3
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-iminobutanoate + H2O
2-oxobutanoate + NH3
show the reaction diagram
2-iminopropanoate + H2O
pyruvate + NH3
show the reaction diagram
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 16% (w/v) PEG 10000, 0.1 M bis-Tris pH 5.3 and 0.1 M ammonium acetate
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography, and Superdex 75 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta(DE3) cells
Show AA Sequence (150 entries)
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