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Information on EC 3.5.5.4 - cyanoalanine nitrilase and Organism(s) Lupinus angustifolius and UniProt Accession Q5QGZ8

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.4 cyanoalanine nitrilase
IUBMB Comments
L-Asparagine is formed as an intermediate. cf. EC 4.2.1.65, 3-cyanoalanine hydratase and EC 3.5.1.1, asparaginase.
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This record set is specific for:
Lupinus angustifolius
UNIPROT: Q5QGZ8
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Word Map
The taxonomic range for the selected organisms is: Lupinus angustifolius
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
nitrilase 4, cyanoalanine hydratase, 3-cyanoalanine nitrilase, nit4a, cyanoalanine nitrilase, beta-cyanoalanine nitrilase, nit4b, nit4b2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
beta-cyanoalanine nitrilase
-
-
-
-
NIT4A
-
isoform
NIT4B
-
isoform
nitrilase 4
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of nitriles
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3-cyano-L-alanine aminohydrolase
L-Asparagine is formed as an intermediate. cf. EC 4.2.1.65, 3-cyanoalanine hydratase and EC 3.5.1.1, asparaginase.
CAS REGISTRY NUMBER
COMMENTARY hide
85638-44-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-cyano-L-alanine + H2O
L-aspartate + NH3
show the reaction diagram
-
enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 4:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate
-
?
phenyl-3-propionitrile + H2O
phenyl-3-propionic acid + NH3
show the reaction diagram
-
-
-
?
3-cyano-L-alanine + H2O
L-aspartate + NH3
show the reaction diagram
-
enzyme has additional hydratase activity for 3-cyano-L-alanine, ratio of hydratase activity to nitrilase activity is 3.3:1. L-asparagine and L-aspartate are formed at the same time, asparagine is not an intermediate
-
?
beta-cyano-L-alanine + H2O
L-aspartate + NH3
show the reaction diagram
-
L-asparagine is formed as an intermediate
-
-
?
phenyl-3-propionitrile + H2O
phenyl-3-propionic acid + NH3
show the reaction diagram
-
-
-
?
additional information
?
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.18
3-Cyano-L-alanine
pH 8.0, 37°C
1.18
3-Cyano-L-alanine
pH 8.0, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.1
substrate phenyl-3-propionic acid, pH 8.0, 37°C
143.7
substrate 3-cyano-L-alanine, pH 8.0, 37°C
0.048
substrate phenyl-3-propionic acid, pH 8.0, 37°C
143.7
substrate 3-cyano-L-alanine, pH 8.0, 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isoform LaNIT4A, in addition to nitrilase activity, enzyme shows cyanoalanine hydratase activity
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NRL4A_LUPAN
349
0
37762
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
x * 38000, SDS-PAGE
38000
x * 38000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 38000, SDS-PAGE
?
x * 38000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Piotrowski, M.; Volmer, J.J.
Cyanide metabolism in higher plants: cyanoalanine hydratase is a NIT4 homolog
Plant Mol. Biol.
61
111-122
2006
Lupinus angustifolius (Q3LRV4), Lupinus angustifolius (Q5QGZ8)
Manually annotated by BRENDA team
Janowitz, T.; Trompetter, I.; Piotrowski, M.
Evolution of nitrilases in glucosinolate-containing plants
Phytochemistry
70
1680-1686
2009
Allium cepa, Arabidopsis thaliana, Brassica rapa, Capsella rubella, Carica papaya, Curcuma longa, Glycine max, Hordeum vulgare, Lactuca sativa, Linum usitatissimum, Lotus japonicus, Lupinus angustifolius, Manihot esculenta, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Pinus taeda, Populus trichocarpa, Ricinus communis, Saccharum officinarum, Selaginella moellendorffii, Solanum lycopersicum, Sorghum bicolor, Tarenaya spinosa, Triticum aestivum, Tropaeolum majus, Vitis vinifera, Zea mays
Manually annotated by BRENDA team