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Information on EC 3.5.5.1 - nitrilase and Organism(s) Talaromyces marneffei and UniProt Accession B6Q5I3

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.5 In nitriles
                3.5.5.1 nitrilase
IUBMB Comments
Acts on a wide range of aromatic nitriles including (indol-3-yl)acetonitrile, and also on some aliphatic nitriles, and on the corresponding acid amides. cf. EC 4.2.1.84 nitrile hydratase.
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This record set is specific for:
Talaromyces marneffei
UNIPROT: B6Q5I3
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Word Map
The taxonomic range for the selected organisms is: Talaromyces marneffei
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
nitrilase, nitrilase 1, cyc705, nitrilase bll6402, nlase, nitrile aminohydrolase, benzonitrilase a, nitras-atii, nitmc-fb, nitrilase i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetonitrilase
-
-
-
-
Arylacetonitrilase
-
-
-
-
benzonitrilase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
nitrile aminohydrolase
Acts on a wide range of aromatic nitriles including (indol-3-yl)acetonitrile, and also on some aliphatic nitriles, and on the corresponding acid amides. cf. EC 4.2.1.84 nitrile hydratase.
CAS REGISTRY NUMBER
COMMENTARY hide
157297-79-5
Arabidopsis thaliana columbiana and Lansberg gene NIT2
157575-01-4
Arabidopsis thaliana columbiana gene NIT3
157575-02-5
Arabidopsis thaliana columbiana gene NIT4
205331-43-7
Arabidopsis thaliana nit2 isoenzyme2
205394-78-1
Arabidopsis thaliana nit3 isoenzyme3
205394-80-5
Arabidopsis thaliana nit1 isoenzyme1
9024-90-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
show the reaction diagram
-
-
-
?
3-chlorobenzonitrile + 2 H2O
3-chlorobenzoate + NH3
show the reaction diagram
-
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
show the reaction diagram
-
-
-
?
4-chlorobenzonitrile + 2 H2O
4-chlorobenzoate + NH3
show the reaction diagram
-
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
show the reaction diagram
-
-
-
?
benzonitrile + 2 H2O
benzoate + NH3
show the reaction diagram
-
-
-
?
phenylacetonitrile + 2 H2O
phenylacetate + NH3
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-cyanopyridine + 2 H2O
pyridine 2-carboxylic acid + NH3
show the reaction diagram
-
-
-
?
3-chlorobenzonitrile + 2 H2O
3-chlorobenzoate + NH3
show the reaction diagram
-
-
-
?
3-cyanopyridine + 2 H2O
pyridine 3-carboxylic acid + NH3
show the reaction diagram
-
-
-
?
4-chlorobenzonitrile + 2 H2O
4-chlorobenzoate + NH3
show the reaction diagram
-
-
-
?
4-cyanopyridine + 2 H2O
pyridine 4-carboxylic acid + NH3
show the reaction diagram
-
-
-
?
benzonitrile + 2 H2O
benzoate + NH3
show the reaction diagram
-
-
-
?
phenylacetonitrile + 2 H2O
phenylacetate + NH3
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.7
2-Cyanopyridine
pH 8.0, 30°C, recombinant enzyme
11.1
3-chlorobenzonitrile
pH 8.0, 30°C, recombinant enzyme
34.4
3-Cyanopyridine
pH 8.0, 30°C, recombinant enzyme
2.6
4-chlorobenzonitrile
pH 8.0, 30°C, recombinant enzyme
15.4
4-cyanopyridine
pH 8.0, 30°C, recombinant enzyme
7.9
Benzonitrile
pH 8.0, 30°C, recombinant enzyme
9.1
phenylacetonitrile
pH 8.0, 30°C, recombinant enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.24
recombinant enzyme expressed without GroEL/ES, pH 8.0, 30°C
0.7
recombinant enzyme coexpressed with GroEL/ES, pH 8.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.7
recombinant enzyme in cell-free extract, room temperature, 2 h, 70% activity remaining at pH 6.5 and pH 8.7, profile overview
718670
7
purified recombinant enzyme, room temperature, 2 h, completely stable
718670
9.5
and above, purified recombinant enzyme, room temperature, 2 h, no activity remaining
718670
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 35
recombinant enzyme in cell-free extract, pH 8.0, 1 h, stable
40
recombinant enzyme in cell-free extract, pH 8.0, 1 h, 20% activity remaining
55
and above, recombinant enzyme in cell-free extract, pH 8.0, 1 h, no activity remaining
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme 4fold from Escherichia coli strain BL21-Gold (DE3), co-purifiication with GroEL
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli strain BL21-Gold (DE3), co-expression of GroEL/ES
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Petrickova, A.; Vesela, A.B.; Kaplan, O.; Kubac, D.; Uhnakova, B.; Malandra, A.; Felsberg, J.; Rinagelova, A.; Weyrauch, P.; K?en, V.; Bezouska, K.; Martinkova, L.
Purification and characterization of heterologously expressed nitrilases from filamentous fungi
Appl. Microbiol. Biotechnol.
93
1553-1561
2012
Fusarium verticillioides (Q19A54), Fusarium verticillioides, Talaromyces marneffei (B6Q5I3), Talaromyces marneffei ATCC 18224 (B6Q5I3), Talaromyces marneffei ATCC 18224
Manually annotated by BRENDA team