Information on EC 3.5.4.8 - aminoimidazolase

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The expected taxonomic range for this enzyme is: Clostridium cylindrosporum

EC NUMBER
COMMENTARY hide
3.5.4.8
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RECOMMENDED NAME
GeneOntology No.
aminoimidazolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-aminoimidazole + H2O = imidazol-4-one + NH3
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amidine hydrolysis
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Purine metabolism
-
-
purine nucleobases degradation I (anaerobic)
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purine nucleobases degradation II (anaerobic)
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purine metabolism
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SYSTEMATIC NAME
IUBMB Comments
4-aminoimidazole aminohydrolase
Requires Fe2+. This enzyme forms part of the xanthine-degradation pathway in some bacteria. The product of the reaction, imidazol-4-one, can be converted non-enzymically into formiminoglycine. An enzyme has been identified in Clostridium cylindrosporum that can perform this hydrolysis reaction [2,3].
CAS REGISTRY NUMBER
COMMENTARY hide
9025-17-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain HC1
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Manually annotated by BRENDA team
strain HC1
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-aminoimidazole + H2O
formiminoglycine + NH3 + unidentified product
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-aminoimidazole + H2O
formiminoglycine + NH3 + unidentified product
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
restores activity of EDTA treated enzyme to 83% of the original activity
Fe2+
-
divalent cation required for activity
Mn2+
-
restores activity of EDTA treated enzyme to 60% of the original activity
Ni2+
-
restores activity of EDTA treated enzyme to 23% of the original activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-Amino-1,2,4-triazole
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-
4-Hydroxy-1,2,3-triazole
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less effective inhibitor
Co2+
-
addition of 0.002 M reduces activity by 50% or more
histamine
-
less effective inhibitor
Mg2+
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addition of 0.002 M reduces activity by 50% or more
Mn2+
-
addition of 0.002 M reduces activity by 50% or more
Zn2+
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addition of 0.002 M reduces activity by 50% or more
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.8
4-aminoimidazole
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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in triethanolamine hydrochloride
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable in absence of reducing agents, completely inactive without cysteine, cysteine, sodium hydrosulfite, glutathione, sodium thioglycolate and ascorbic acid stabilizes enzyme activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE