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Information on EC 3.5.4.5 - cytidine deaminase and Organism(s) Sporosarcina psychrophila and UniProt Accession Q9S3M0

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.5 cytidine deaminase
IUBMB Comments
Contains zinc. Catalyses the deamination of cytidine and 2'-deoxycytidine with similar efficiencies. The enzyme, which is widely distributed among organisms, is involved in salvage of both exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
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This record set is specific for:
Sporosarcina psychrophila
UNIPROT: Q9S3M0
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Word Map
The taxonomic range for the selected organisms is: Sporosarcina psychrophila
The enzyme appears in selected viruses and cellular organisms
Synonyms
cytidine deaminase, activation-induced cytidine deaminase, aicda, deoxycytidine deaminase, cr deaminase, cyd deaminase, t-cda, cytosine nucleoside deaminase, cytidine aminohydrolase, yeast cytidine deaminase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
activation-induced cytidine deaminase
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-
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AICDA
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-
-
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Cytidine aminohydrolase
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-
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cytosine nucleoside deaminase
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-
-
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DCD
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-
-
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dCDA
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Deamination
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amidine hydrolysis
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
cytidine/2'-deoxycytidine aminohydrolase
Contains zinc. Catalyses the deamination of cytidine and 2'-deoxycytidine with similar efficiencies. The enzyme, which is widely distributed among organisms, is involved in salvage of both exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis.
CAS REGISTRY NUMBER
COMMENTARY hide
37259-56-6
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9025-06-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
deoxycytidine + H2O
deoxyuridine + NH3
show the reaction diagram
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-
-
?
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 70
6°C: 45% of maximal activity, 70°C: 35% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2
chromatofocusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CDD_SPOPS
136
0
14600
Swiss-Prot
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14600
4 * 14600, calculation from nucleotide sequence
15000
4 * 15000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
72
30 min, irreversible denaturation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
irreversible denaturation at 72°C after 30 min
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cambi, A.; Vincenzetti, S.; De Sanctis, G.; Neuhard, J.; Natalini, P.; Vita, A.
Cytidine deaminase from two extremophilic bacteria: cloning, expression and comparison of their structural stability
Protein Eng.
14
807-813
2001
Bacillus subtilis, [Bacillus] caldolyticus (Q9R2S1), [Bacillus] caldolyticus, Sporosarcina psychrophila (Q9S3M0), Sporosarcina psychrophila
Manually annotated by BRENDA team