Information on EC 3.5.4.45 - melamine deaminase

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The expected taxonomic range for this enzyme is: Acidovorax citrulli

EC NUMBER
COMMENTARY hide
3.5.4.45
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RECOMMENDED NAME
GeneOntology No.
melamine deaminase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ammeline + H2O = ammelide + NH3
show the reaction diagram
(2)
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melamine + H2O = ammeline + NH3
show the reaction diagram
(1)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
melamine degradation
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Atrazine degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
melamine aminohydrolase
The enzyme, isolated from the bacterium Acidovorax citrulli, performs the deamination of melamine 15-fold faster than the deamination of ammeline. It also has activity with 2-chloro-4,6-diamino-s-triazine, but has no activity toward halo-substituted triazine ring compounds such as atrazine (cf. EC 3.8.1.8, atrazine chlorohydrolase).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-chloro-4,6-diamino-s-triazine + H2O
2-chloro-4-amino-6-hydroxy-s-triazine + NH3
show the reaction diagram
aminoatrazine + H2O
hydroxyatrazine + NH3
show the reaction diagram
ammeline + H2O
ammelide + NH3
show the reaction diagram
melamine + H2O
ammeline + NH3
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-chloro-4,6-diamino-s-triazine
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up to 50% inhibtion of melamine turnover
aminoatrazine
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up to 71% inhibtion of melamine turnover
ammeline
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up to 50% inhibtion of melamine turnover
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.017
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substrate 2-chloro-4,6-diamino-s-triazine, pH not specified in the publication, 22°C; substrate aminoatrazine, pH not specified in the publication, 22°C
0.135
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substrate ammeline, pH not specified in the publication, 22°C
0.245
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substrate melamine, pH not specified in the publication, 22°C
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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