Information on EC 3.5.4.43 - hydroxydechloroatrazine ethylaminohydrolase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.4.43
-
RECOMMENDED NAME
GeneOntology No.
hydroxydechloroatrazine ethylaminohydrolase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydroxyatrazine + H2O = N-isopropylammelide + ethylamine
show the reaction diagram
Involved in a pathway by which the herbicide atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, is degraded in bacteria via N-isopropylammelide, 2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of C-N bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Atrazine degradation
-
-
atrazine degradation I (aerobic)
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
degradation of aromatic, nitrogen containing compounds
-
-
SYSTEMATIC NAME
IUBMB Comments
hydroxyatrazine ethylaminohydrolase
Contains Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.
CAS REGISTRY NUMBER
COMMENTARY hide
188496-88-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
isolated from Manfredi soil in Argentina used to grow corn and are treated with atrazine for weed control during 3 years, gene atzB
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Clavibacter michiganese
-
-
-
Manually annotated by BRENDA team
Clavibacter michiganese ATZ1
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
use of atrazine as sole nitrogen source and as sole carbon source. Comparison of degradation products with those from Pseudomonas sp. and Pseudoaminobacter sp.
-
-
Manually annotated by BRENDA team
use of atrazine as sole nitrogen source and as sole carbon source. Comparison of degradation products with those from Pseudomonas sp. and Nocardiodes sp.
-
-
Manually annotated by BRENDA team
Pseudoaminobacter sp.
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-di(N-isobutylamino)-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-di(N-sec-butylamino)-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-di(N-tert-butylamino)-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-diamino-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-dichloro-6-amino-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-diethylamino-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2,4-dimethylamino-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-(N-ethyl-N-methylamino)-4-ethylamino-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-(N-ethylamino)-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-amino-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-amino-4-chloro-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-amino-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-amino-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-amino-6-(N-ethylamino)-4-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-azido-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-chloro-4-amino-6-hydroxy-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-chloro-4-amino-6-hydroxy-s-triazine + H2O
4-amino-2,6-dihydroxy-s-triazine + HCl
show the reaction diagram
-
-
ammelide is the sole reaction product
-
?
2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-cyano-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-fluoro-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-hydroxy-4,6-di(N-hydroxyethylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-hydroxy-4-(N-ethylamino)-6-(N-isopropylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-hydroxy-4-(N-isopropylamino)-6-(N-(3-methoxypropyl)amino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-isopropylamino-4-ethylamino-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
2-mercapto-4-isopropylamino-6-ethylamino-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
4,6-bis-isopropylamine-1H-triazin-2-one + H2O
?
show the reaction diagram
-
-
-
-
?
4,6-bisethylamino-1H-[1,3,5]triazine-2-one + H2O
?
show the reaction diagram
-
-
-
-
?
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
N-isopropylammelide + ethylamine
show the reaction diagram
4-(ethylamino)-6-(propan-2-ylamino)-1,3,5-triazin-2-ol + H2O
6-(propan-2-ylamino)-1,3,5-triazine-2,4-diol + ethanamine
show the reaction diagram
-
-
-
-
ir
4-(N-ethylamino)-6-hydroxy-2-(N-methylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
4-amino-6-ethylamino-[1,3,5]triazine-2-ol + H2O
6-amino-[1,3,5]triazine-2,4-diol + ethylamine
show the reaction diagram
-
-
-
-
?
4-amino-6-isopropylamino-[1,3,5]triazine-2-ol + H2O
6-amino-[1,3,5]triazine-2,4-diol + isopropylamine
show the reaction diagram
-
-
-
-
?
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
show the reaction diagram
hydroxyatrazine + H2O
N-isopropylammelide + ethylamine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H2O
?
show the reaction diagram
4-(ethylamino)-6-(propan-2-ylamino)-1,3,5-triazin-2-ol + H2O
6-(propan-2-ylamino)-1,3,5-triazine-2,4-diol + ethanamine
show the reaction diagram
-
-
-
-
ir
hydroxyatrazine + H2O
N-isopropyl ammelide + ethanamine
show the reaction diagram
additional information
?
-
-
the isolated strain is capable of degrading atrazine. Displacement of the three substituents on the s-triazine ring is mediated by three enzymatic steps encoded by the genes atzA, atzB, and atzC. AtzB catalyzes hydroxyatrazine deamidation, yielding N-isopropylammelide
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
72% inhibition, not due to metal removal
8-Hydroxyquinoline-5-sulfonic acid
-
-
Chelex-100 resin
-
-
dipicolinic acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.05
-
purified recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
isoelectric focusing, recombinant AtzB
5.7
-
sequence calculation
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000
-
2 * 52000, recombinant AtzB, SDS-PAGE
105000
-
recombinant AtzB, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 52000, recombinant AtzB, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme 6.6fold from Escherichia coli strain DH5alpha to homogeneity by ammonium sulfate fractionation, hydroxyapatite chromatography, and gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene atzB, cloned from soil isolated strain, DNA and amino acid sequence determination and analysis, sequence comparison
-
gene atzB, expression in Escherichia coli strain DH5alpha
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
exposure to atrazine results in up to 16fold increase in expression
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
-
degradation of atrazine to non-phytotoxic metabolites
degradation
Show AA Sequence (606 entries)
Please use the Sequence Search for a specific query.