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Information on EC 3.5.4.4 - adenosine deaminase and Organism(s) Schizosaccharomyces pombe and UniProt Accession Q9P6I7

for references in articles please use BRENDA:EC3.5.4.4
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EC Tree
IUBMB Comments
The enzyme, found in a wide variety of microorganisms, plants, invertebrates, and animals, plays a role in purine metabolism.
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This record set is specific for:
Schizosaccharomyces pombe
UNIPROT: Q9P6I7
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The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
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adenosine
+
H2O
=
inosine
+
NH3
+
=
+
Synonyms
adenosine deaminase, adenosine deaminase 2, adenosine aminohydrolase, adenosine deaminase 1, adaii, pvada, mj1541, ciada, sco4901, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Adenosine aminohydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Deamination
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
adenosine aminohydrolase
The enzyme, found in a wide variety of microorganisms, plants, invertebrates, and animals, plays a role in purine metabolism.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-93-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenine + H2O
hypoxanthine + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
2.0% of the activity of adenine
-
-
?
adenosine 3'-phosphate + H2O
inosine 3'-phosphate + NH3
show the reaction diagram
4.5% of the activity of adenine
-
-
?
ATP + H2O
ITP + NH3
show the reaction diagram
3.3% of the activity of adenine
-
-
?
N6-isopentenyladenosine + H2O
?
show the reaction diagram
1.9% of the activity of adenine
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7
0.2 M phosphate buffer, 33°C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33
0.2 M phosphate buffer, pH 6.7
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
this enzyme catalyses the reaction of EC 3.5.4.2 and EC 3.5.4.4; wild-type 972 h
SwissProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
activity of purified protein is stable for 1-2 weeks when stored at 4°C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20°C with 50% (v/v) glycerol and at -80°C with 10-20% (v/v) glycerol or as freeze-dried powder
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli BL21 (DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pospisilova, H.; Sebela, M.; Novak, O.; Frebort, I.
Hydrolytic cleavage of N6-substituted adenine derivatives by eukaryotic adenine and adenosine deaminases
Biosci. Rep.
28
335-347
2008
Saccharomyces cerevisiae (P53909), Saccharomyces cerevisiae, Arabidopsis thaliana (Q8LPL7), Arabidopsis thaliana, Schizosaccharomyces pombe (Q9P6I7), Schizosaccharomyces pombe
Manually annotated by BRENDA team