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Information on EC 3.5.4.34 - tRNAAla(adenine37) deaminase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P53065

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IUBMB Comments
The enzyme deaminates adenosine37 to inosine in eukaryotic tRNAAla . tRNA editing is strictly dependent on Mg2+ .
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P53065
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
adenine37 in tRNAAla
+
=
hypoxanthine37 in tRNAAla
+
Synonyms
adat1, hadat1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
tRNAAla(adenine37) aminohydrolase
The enzyme deaminates adenosine37 to inosine in eukaryotic tRNAAla [1]. tRNA editing is strictly dependent on Mg2+ [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenine37 in tRNAAla + H2O
hypoxanthine37 in tRNAAla + NH3
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenine37 in tRNAAla + H2O
hypoxanthine37 in tRNAAla + NH3
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
the KCl optimum for A37–I37 conversion by Tad1p is 25 mM
Mg2+
tRNA editing is strictly magnesium dependent with an optimal concentration between 2–3 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KCl
high salt concentrations (above 100 mM KCl) inhibit the reaction
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000006
adenine37 in tRNAAla
30°C, pH 7,9
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0705
adenine37 in tRNAAla
30°C, pH 7,9
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
the TAD1 gene is not essential in yeast under optimal growth conditions at 30°C
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
mutant strains in which the TAD1 gene is disrupted are viable but lack Tad1p enzyme activity and their tRNAAla is not modified at position A37
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gerber, A.; Grosjean, H.; Melcher, T.; Keller, W.
Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2
EMBO J.
17
4780-4789
1998
Saccharomyces cerevisiae (P53065)
Manually annotated by BRENDA team