Information on EC 3.5.4.34 - tRNAAla(adenine37) deaminase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.4.34
-
RECOMMENDED NAME
GeneOntology No.
tRNAAla(adenine37) deaminase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
adenine37 in tRNAAla + H2O = hypoxanthine37 in tRNAAla + NH3
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
tRNAAla(adenine37) aminohydrolase
The enzyme deaminates adenosine37 to inosine in eukaryotic tRNAAla [1]. tRNA editing is strictly dependent on Mg2+ [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
the TAD1 gene is not essential in yeast under optimal growth conditions at 30°C
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenine37 in tRNAAla + H2O
hypoxanthine37 in tRNAAla + NH3
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenine37 in tRNAAla + H2O
hypoxanthine37 in tRNAAla + NH3
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
the KCl optimum for A37–I37 conversion by Tad1p is 25 mM
Mg2+
tRNA editing is strictly magnesium dependent with an optimal concentration between 2–3 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KCl
high salt concentrations (above 100 mM KCl) inhibit the reaction
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000006
adenine37 in tRNAAla
30°C, pH 7,9
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0705
adenine37 in tRNAAla
Saccharomyces cerevisiae
P53065
30°C, pH 7,9
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
the enzyme is expressed ubiquitously in human tissues
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45350
x * 45350, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 45350, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Pichia pastoris
expression in Pichia pastoris
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
mutant strains in which the TAD1 gene is disrupted are viable but lack Tad1p enzyme activity and their tRNAAla is not modified at position A37