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Information on EC 3.5.4.33 - tRNA(adenine34) deaminase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P47058

for references in articles please use BRENDA:EC3.5.4.33
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EC Tree
IUBMB Comments
The enzyme is involved in editing of tRNA. The active site contains Zn2+ .
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P47058 not found.
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Word Map
  • 3.5.4.33
  • deamination
  • wobble
  • anticodons
  • heterodimeric
  • a-to-i
  • deaminases
  • chloroplast
  • decode
  • intellectual
  • trna-specific
  • disability
  • trypanosoma
  • trnaarg
  • trypanosomatids
  • adats
  • base-pairing
  • cytidine-to-uridine
  • post-transcriptionally
  • brucei
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
adenine34 in tRNA
+
=
hypoxanthine34 in tRNA
+
Synonyms
adat2/3, tada protein, trna:adenosine 34 deaminase, attada, sptad, adat2-adat3 complex, trna a:34 deaminase, trna adenosine deaminase arginine, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ADAT2-ADAT3 complex
P47058; Q9URQ3
-
tRNA:A34 deaminase
P47058; Q9URQ3
-
tRNA:adenosine 34 deaminase
P47058; Q9URQ3
-
SYSTEMATIC NAME
IUBMB Comments
tRNA(adenine34) aminohydrolase
The enzyme is involved in editing of tRNA. The active site contains Zn2+ [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
show the reaction diagram
adenine34 in tRNAAla(AGC) + H2O
hypoxanthine34 in tRNAAla(IGC) + NH3
show the reaction diagram
P47058; Q9URQ3
-
-
-
?
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
show the reaction diagram
adenine34 in tRNASer(AGA) + H2O
hypoxanthine34 in tRNASer(IGA) + NH3
show the reaction diagram
P47058; Q9URQ3
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenine34 in tRNA + H2O
hypoxanthine34 in tRNA + NH3
show the reaction diagram
adenine34 in tRNAArg + H2O
hypoxanthine34 in tRNAArg + NH3
show the reaction diagram
P47058; Q9URQ3
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
P47058; Q9URQ3
optimum concentration2-5 mM, the enzyme does not require any cofactor, except magnesium ions, to deaminate adenosine 34 efficiently in tRNA. The dependence of the enzymatic reaction on magnesium ions probably reflects the need for a correct tRNA architecture
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
P47058; Q9URQ3
5 mM abolishes activity up to 70%
4,7-phenanthroline
P47058; Q9URQ3
5 mM abolishes activity up to 70%
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000023
adenine34 in tRNASer(AGA)
P47058; Q9URQ3
pH and temperature not specified in the publication
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
P47058; Q9URQ3
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
P47058: TAD2 and Q9URQ3: TAD3
P47058; Q9URQ3
UniProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
P47058; Q9URQ3
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
P47058; Q9URQ3
the heterodimeric enzyme consists of two sequence-related subunits (Tad2p/ADAT2 and Tad3p/ADAT3), both of which contain cytidine deaminase motifs
homodimer
P47058; Q9URQ3
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E56A
P47058; Q9URQ3
mutation in subunit Tad2, mutant enzyme has no deaminase activity in vitro
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
P47058; Q9URQ3
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Elias, Y.; Huang, R.H.
Biochemical and structural studies of A-to-I editing by tRNA:A34 deaminases at the wobble position of transfer RNA
Biochemistry
44
12057-12065
2005
Agrobacterium tumefaciens (A9CK16), Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Saccharomyces cerevisiae (P47058 and Q9URQ3), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (P47058 and Q9URQ3)
Manually annotated by BRENDA team
Auxilien, S.; Crain, P.F.; Trewyn, R.W.; Grosjean, H.
Mechanism, specificity and general properties of the yeast enzyme catalysing the formation of inosine 34 in the anticodon of transfer RNA
J. Mol. Biol.
262
437-458
1996
Saccharomyces cerevisiae (P47058 and Q9URQ3), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (P47058 and Q9URQ3)
Manually annotated by BRENDA team
Gerber, A.P.; Keller, W.
An adenosine deaminase that generates inosine at the wobble position of tRNAs
Science
286
1146-1149
1999
Saccharomyces cerevisiae (P47058 and Q9URQ3), Saccharomyces cerevisiae ATCC 204508 (P47058 and Q9URQ3)
Manually annotated by BRENDA team