Information on EC 3.5.4.32 - 8-oxoguanine deaminase

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The expected taxonomic range for this enzyme is: Pseudomonas aeruginosa

EC NUMBER
COMMENTARY hide
3.5.4.32
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RECOMMENDED NAME
GeneOntology No.
8-oxoguanine deaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
8-oxoguanine + H2O = urate + NH3
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
8-oxoguanine aminohydrolase
Zn2+ is bound in the active site. 8-Oxoguanine is formed via the oxidation of guanine within DNA by reactive oxygen species. If uncorrected, this modification leads to the incorporation of 8-oxoG:A mismatches and eventually to G:C to T:A transversions.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
identified from environmental DNA sequencing efforts in the Sargasso Sea
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
8-oxoguanine + H2O
8-oxoxanthine + NH3
show the reaction diagram
8-oxoguanine + H2O
urate + NH3
show the reaction diagram
ammeline + H2O
?
show the reaction diagram
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-
?
guanine + H2O
xanthine + NH3
show the reaction diagram
isocytosine + H2O
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
8-oxoguanine + H2O
8-oxoxanthine + NH3
show the reaction diagram
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8-oxoguanine (8-oxoG), which is formed by the oxidation of guanine within DNA via reactive oxygen species. If uncorrected, this modification leads to the incorporation of 8-oxoG:A mismatches and eventually to G:C to T:A transversions
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?
8-oxoguanine + H2O
urate + NH3
show the reaction diagram
Q9I6Z0
8-oxoguanine (8-oxoG), which is formed by the oxidation of guanine within DNA via reactive oxygen species. If uncorrected, this modification leads to the incorporation of 8-oxoG:A mismatches and eventually to G:C to T:A transversions
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
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a single zinc ion is bound in the active site. The divalent cation is coordinated to two histidine residues (H93 and H95)
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1 - 2.6
guanine
1.6 - 1.8
isocytosine
additional information
additional information
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.3 - 52
guanine
3.9 - 62
isocytosine
additional information
additional information
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20 - 270
8-oxoguanine
4165
0.4 - 52
guanine
199
2.2 - 392
isocytosine
6539
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
the crystals of the SeMet Pa0142 homologue complexed with Zn2+ are grown by vapor diffusion at room temperature
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
codon-optimized for expression in Escherichia coli
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