Information on EC 3.5.4.22 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
3.5.4.22
-
RECOMMENDED NAME
GeneOntology No.
1-pyrroline-4-hydroxy-2-carboxylate deaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-pyrroline-4-hydroxy-2-carboxylate + H2O = 2,5-dioxopentanoate + NH3
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of amidines
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
-
-
trans-4-hydroxy-L-proline degradation II
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-
SYSTEMATIC NAME
IUBMB Comments
1-pyrroline-4-hydroxy-2-carboxylate aminohydrolase (decyclizing)
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CAS REGISTRY NUMBER
COMMENTARY hide
9054-77-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-pyrroline-4-hydroxy-2-carboxylate + H2O
2,5-dioxopentanoate + NH3
show the reaction diagram
D-1-pyrroline-4-hydroxy-2-carboxylate + H2O
2,5-dioxopentanoate + NH3
show the reaction diagram
-
-
-
-
ir
L-1-pyrroline-4-hydroxy-2-carboxylate + H2O
2,5-dioxopentanoate + NH3
show the reaction diagram
-
-
-
-
ir
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-pyrroline-4-hydroxy-2-carboxylate + H2O
2,5-dioxopentanoate + NH3
show the reaction diagram
L-1-pyrroline-4-hydroxy-2-carboxylate + H2O
2,5-dioxopentanoate + NH3
show the reaction diagram
-
-
-
-
ir
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
-
0.01 mM
Cu2+
-
0.01 mM
Hg2+
-
0.01 mM
L-2-oxo-3-deoxyarabinonate
-
competitive inhibition
-
p-mercuribenzoate
-
1 mM, 85% inhibition
pyruvate
-
competitive inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.693 - 1.4
1-pyrroline-4-hydroxy-2-carboxylate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.19
1-pyrroline-4-hydroxy-2-carboxylate
Pseudomonas putida
-
at pH 8.0 and 30°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.592
L-2-oxo-3-deoxyarabinonate
-
at pH 8.0 and 30°C
-
0.492
pyruvate
-
at pH 8.0 and 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
below pH 6 rapid decomposition of substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Brucella melitensis biotype 2 (strain ATCC 23457)
Brucella melitensis biotype 2 (strain ATCC 23457)
Rhizobium meliloti (strain 1021)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35000
-
8 * 35000, His6-tagged protein, SDS-PAGE
35342
-
8 * 35342, calculated from amino acid sequence
62000
-
ultracentrifugation
275000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homooctamer
additional information
-
part of the glucarate- and hydroxyproline-induced KGSA dehydrogenase
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, fraction II, at least one month
-
-15°C, fraction IV, at least one month
-
25°C,fraction IV, at least three hours
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha cells
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