Information on EC 3.5.4.21 - creatinine deaminase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.5.4.21
-
RECOMMENDED NAME
GeneOntology No.
creatinine deaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
creatinine + H2O = N-methylhydantoin + NH3
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of cyclic amidines
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
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creatinine degradation II
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Metabolic pathways
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creatinine degradation
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SYSTEMATIC NAME
IUBMB Comments
creatinine iminohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37289-15-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
Flavobacterium filamentosum
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
-
-
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
unclassified bacterium BN 11
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-methylcytosine + H2O
3-methyluracil + NH3
show the reaction diagram
Flavobacterium filamentosum
-
-
-
-
?
5-fluorocytosine + H2O
5-fluorouracil + NH3
show the reaction diagram
Flavobacterium filamentosum
-
-
-
-
?
5-methylcytosine + H2O
5-methyluracil + NH3
show the reaction diagram
Flavobacterium filamentosum
-
-
-
-
?
creatinine + H2O
N-methylhydantoin + NH3
show the reaction diagram
cytosine + H2O
uracil + NH3
show the reaction diagram
Flavobacterium filamentosum
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
creatinine + H2O
N-methylhydantoin + NH3
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
Flavobacterium filamentosum
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-
Zn2+
Flavobacterium filamentosum
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-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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1 mM, 4.7% inhibition
2,2'-dipyridyl
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1 mM, 4.5% inhibition
Ca2+
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1 mM, 31% inhibition
Cd2+
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1 mM, 100% inhibition
Co2+
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1 mM, 78% inhibition
EDTA
-
1 mM, 9% inhibition
Fe2+
-
1 mM, 100% inhibition
Mg2+
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1 mM, 30% inhibition
Mn2+
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1 mM, 58% inhibition
NaF
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enzyme dialyzed against 20 mM became 78% inhibited
p-mercuribenzoate
Zn2+
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1 mM, 100% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.42
5-fluorocytosine
Flavobacterium filamentosum
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-
0.15 - 18
creatinine
0.62
cytosine
Flavobacterium filamentosum
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00001
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NH4Cl added to culture medium
0.0001
0.00015
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N-methylhydantoin added to culture medium
0.0002
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hydantoin added to culture medium
0.0004
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cytosine added to culture medium
0.00043
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creatinine added to culture medium
0.0006
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hydantoin added to culture medium
0.0011
0.0012
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hydantoin added to culture medium
0.0013
0.0014
0.0015
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NH4Cl added to culture medium
0.0016
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NH4Cl added to culture medium
0.0018
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polypeptone added to culture medium
0.0021
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NH4Cl added to culture medium
0.0024
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cytosine added to culture medium
0.0028
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NH4Cl added to culture medium
0.0034
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NH4Cl added to culture medium
0.0051
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creatinine added to culture medium
0.0056
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polypeptone added to culture medium
0.0058
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N-methylhydantoin added to culture medium
0.0062
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polypeptone added to culture medium
0.0064
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NH4Cl added to culture medium
0.0065
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polypeptone added to culture medium
0.0067
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creatinine added to culture medium
0.0072
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polypeptone added to culture medium
0.0092
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cytosine added to culture medium
0.01
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creatinine added to culture medium
0.0112
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creatinine added to culture medium
0.0198
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N-methylhydantoin added to culture medium
0.0216
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creatinine added to culture medium
0.0452
0.0729
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creatinine added to culture medium
0.089
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cytosine added to culture medium
39.8
Flavobacterium filamentosum
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.5
Flavobacterium filamentosum
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7.5 - 8
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immobilized enzyme
8.5 - 8.75
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44300
Flavobacterium filamentosum
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6 * 44300, SDS-PAGE
46310
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two-dimensional gel electrophoresis
47500
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6 * 47500, SDS-PAGE
195000
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ultracentrifugation
260000
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gel filtration
272500
Flavobacterium filamentosum
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gel filtration
285000
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gel filtration, PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
precipitated by addition of ammonim sulfate to 70% saturation, 4C over night
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
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30 min, 13% loss in activity
70
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30 min, 20% activity retained
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 50 mM potassium phosphate buffer, pH 7.65, 50% glycerol, loss in activity 0.038 U/ml per month
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4C, after two months no loss in activity
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5C, 20 mM phosphate buffer, pH 7, 60% saturated ammonium sulfate, at least 6 months
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5C, 20 mM phosphate buffer, pH 7, one week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis