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Information on EC 3.5.4.2 - adenine deaminase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P53909
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3.5.4.2 adenine deaminaseSpecify your search results
Word Map
- 3.5.4.2
- purine
- hypoxanthine
- inosine
- nucleosidase
-
2.4.2.7
-
2\'-deoxycoformycin
- deaminases
-
nickase
-
phosphoribosyltransferases
- guanase
- food industry
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
adenine deaminase, adenine aminohydrolase, adase, aah1p, adenase, pa0148, aaur1117, sgx9403e, sgx9403g, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ADase
-
-
-
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adenase
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-
-
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adenine aminase
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of amidines
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
adenine aminohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
9027-68-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-[(E)-4-hydroxy-3-methylbut-2-enylamino]purine + H2O
?
1.8% of the activity of adenine
-
-
?
6-[(Z)-4-hydroxy-3-methylbut-2-enylamino]purine + H2O
?
1.7% of the activity of adenine
-
-
?
benzyladenine + H2O
hypoxanthine + 1-phenylmethaneamine
0.7% of the activity of adenine
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-
ir
kinetin + H2O
hypoxanthine + 1-furan-2-ylmethaneamine
2.6% of the activity of adenine
-
-
ir
m-topolin + H2O
hypoxanthine + 2-(aminomethyl)phenol
2.5% of the activity of adenine
-
-
ir
N6-isopentenyladenine + H2O
hypoxanthine + 3-methylbut-3-en-1-amine
2.1% of the activity of adenine
-
-
ir
adenine + H2O
?
-
purine salvage pathway and nitrogen metabolism
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-
?
additional information
?
-
-
gene AAH1 is tightly downregulated upon cell entry into the non-proliferating state/quiescence, which is induced by nutrient limitations, the Srb10p cyclin-dependent kinase and its cyclin, Srb11p, regulate AAH1 expression at the transcriptional level, while Saf1p, a previously uncharacterized F-box protein, acts at a post-transcriptional level by promoting degradation of Aah1p, Aah1p expression is controlled by the RAS cAMP pathway, overview
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-
?
adenine + H2O
hypoxanthine + NH3
irreversible deamination, metabolic salvage pathway of purines
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-
ir
kinetin + H2O
?
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
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-
?
kinetin + H2O
?
hydrolysis of adenine compounds, cleavage of cytokinins into inosine and the corresponding side chain amine
-
-
?
N6-(2-isopentenyl)adenine + H2O
hypoxanthine + 3-methylbut-2-en-1-amine
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
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-
?
N6-(2-isopentenyl)adenine + H2O
hypoxanthine + 3-methylbut-2-en-1-amine
hydrolysis of adenine compounds, low activity towards N6-substituted adenines, partial conversion, release of N6-side chain amines
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
adenine + H2O
?
-
purine salvage pathway and nitrogen metabolism
-
-
?
adenine + H2O
hypoxanthine + NH3
irreversible deamination, metabolic salvage pathway of purines
-
-
ir
kinetin + H2O
?
release of hypoxanthine and inosine, release of amine from from the cytokinine side chain
-
-
?
N6-(2-isopentenyl)adenine + H2O
hypoxanthine + 3-methylbut-2-en-1-amine
partial conversion, at endo-side of N6 amino groups, release of N6-side chain amines
-
-
?
additional information
?
-
-
gene AAH1 is tightly downregulated upon cell entry into the non-proliferating state/quiescence, which is induced by nutrient limitations, the Srb10p cyclin-dependent kinase and its cyclin, Srb11p, regulate AAH1 expression at the transcriptional level, while Saf1p, a previously uncharacterized F-box protein, acts at a post-transcriptional level by promoting degradation of Aah1p, Aah1p expression is controlled by the RAS cAMP pathway, overview
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5.5
adenine
0.067 mM adenine in 0.2 M phosphate buffer, 33°C, pH 7.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.34
recombinant protein, adenine as substrate, 0.067 mM in 0.2 M phosphate buffer, 33°C, pH 7.0, adenine most favoured substrate, also hydrolyzes N6-substituted adenines from the cytokinin group of plant hormones, reaction products identified by mass spectroscopy, relative reaction rates given
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
this enzyme catalyses the reaction of EC 3.5.4.2. and EC 3.5.4.4; strain 23344c
SwissProt
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
isolation and analysis of three deletion mutants srb10, srb11 and saf1 (ybr280c) affecting AAH1 expression during post-diauxic growth and in early stationary phase, overview
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
activity highest in potassium phosphate buffer, decreasing activity to 75% in Tris/HCl buffer and to 30% in MOPS buffer
696861
additional information
activity highest in potassium phosphate buffer, decreasing activity to 75% in Tris/HCl buffer and to 30% in MOPS buffer
696861
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
prolonged storage in solution, at -20°C in presence of 50% glycerol, at -80°C in presence of 10-20% glycerol
additional information
prolonged storage in solution, at -20°C in presence of 50% glycerol, at -80°C in presence of 10-20% glycerol
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after dissolving the freeze-dried enzyme a significant decrease od activity is observed within 3-4 h
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
activity of purified protein is stable for 1-2 weeks when stored at 4°C at concentrations above 10 mg/ml with the addition of 20% (v/v) glycerol. Prolonged storage in solution is possible at -20°C with 50% (v/v) glycerol and at -80°C with 10-20% (v/v) glycerol or as freeze-dried powder
at 4°C in potassium buffer above a concentration of 10 mg/ml and in presence of 20% glycerol stable for 1-2 weeks, prolonged storage in solution at -20°C in presence of 50% glycerol and at -80°C in presence of 10-20% glycerol, or as freeze-dried powder
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using Ni2+-nitrilotriacetate (Ni2+-NTA) column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli, pDrive, pet100/D-TOPO, pet151/D-TOPO vectors
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Deeley, M.C.
Adenine deaminase and adenine utilization in Saccharomyces cerevisiae
J. Bacteriol.
174
3102-3110
1992
Saccharomyces cerevisiae
Escusa, S.; Camblong, J.; Galan, J.M.; Pinson, B.; Daignan-Fornier, B.
Proteasome- and SCF-dependent degradation of yeast adenine deaminase upon transition from proliferation to quiescence requires a new F-box protein named Saf1p
Mol. Microbiol.
60
1014-1025
2006
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
Pospisilova, H.; Sebela, M.; Novak, O.; Frebort, I.
Hydrolytic cleavage of N6-substituted adenine derivatives by eukaryotic adenine and adenosine deaminases
Biosci. Rep.
28
335-347
2008
Saccharomyces cerevisiae, Saccharomyces cerevisiae (P53909), Schizosaccharomyces pombe, Schizosaccharomyces pombe (Q9P6I7)
EXTERNAL LINKS (specific for EC-Number 3.5.4.2)
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Release 2023.1 (January 2023)
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