Information on EC 3.5.4.2 - adenine deaminase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.5.4.2
-
RECOMMENDED NAME
GeneOntology No.
adenine deaminase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
adenine + H2O = hypoxanthine + NH3
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of amidines
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-
-
-
PATHWAY
KEGG Link
MetaCyc Link
adenine and adenosine salvage IV
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Metabolic pathways
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Purine metabolism
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SYSTEMATIC NAME
IUBMB Comments
adenine aminohydrolase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Aah1p
Saccharomyces cerevisiae BY4742
-
-
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ADase
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adenase
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adenine aminase
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adenine deaminase
P53909
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adenine deaminase
Saccharomyces cerevisiae 23344c
P53909
-
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adenine deaminase
Q9P6I7
-
NadA
Q8X1T6
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Sgx9403g
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-
CAS REGISTRY NUMBER
COMMENTARY
9027-68-3
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
no activity in other than some bacteria and some lower eukaryotes
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-
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Manually annotated by BRENDA team
Pseudomonas synxantha A3
strain A3
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-
Manually annotated by BRENDA team
strain BY4742, gene AAH1
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-
Manually annotated by BRENDA team
this enzyme catalyses the reaction of EC 3.5.4.2. and EC 3.5.4.4; strain 23344c
SwissProt
Manually annotated by BRENDA team
Saccharomyces cerevisiae 23344c
this enzyme catalyses the reaction of EC 3.5.4.2. and EC 3.5.4.4; strain 23344c
SwissProt
Manually annotated by BRENDA team
Saccharomyces cerevisiae BY4742
strain BY4742, gene AAH1
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-
Manually annotated by BRENDA team
this enzyme catalyses the reaction of EC 3.5.4.2 and EC 3.5.4.4; wild-type 972 h
SwissProt
Manually annotated by BRENDA team
strain J-350P
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-
Manually annotated by BRENDA team
Streptomyces sp. J-350P
strain J-350P
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
deletion of the AAH locus in intact parasites establishes that AAH is not an essential gene and that -/-aah cells are capable of salvaging the same range of purine nucleobases and nucleosides as wild type Leishmania donovani. The aah null mutant is able to infect murine macrophages in vitro and in mice, although the parasite loads in both model systems are modestly reduced compared with wild type infections
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
-
no dialyzable cofactors required
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Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0231
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2'-Deoxycoformycin
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pH 7.0, 25°C
0.00013
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6-Chloropurine
-
pH 7.5, 30°C
4
-
hypoxanthine
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competitive
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
8.5
-
pH 5: about 30% of maximal activity, pH 8.5: about 20% of maximal activity
6
8
-
potassium phosphate buffer, maximal activity pH 7
7
9
-
Tris-HCl buffer, maximal activity pH 9
7.5
9
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glycine-NaOH buffer, maximal activity pH 7
PDB
SCOP
CATH
ORGANISM
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Arthrobacter aurescens (strain TC1)
Enterococcus faecalis (strain ATCC 700802 / V583)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Western blot analysis establishes that AAH is expressed in both life cycle stages of Leishmania donovani
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