Information on EC 3.5.4.15 - guanosine deaminase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.5.4.15
-
RECOMMENDED NAME
GeneOntology No.
guanosine deaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
guanosine + H2O = xanthosine + NH3
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Deamination
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
guanosine nucleotides degradation I
-
-
purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
guanosine aminohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9067-85-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Col-0
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain No. 149
-
-
Manually annotated by BRENDA team
strain No. 149
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxyguanosine + H2O
2'-deoxyxanthosine + NH3
show the reaction diagram
8-azaguanosine + H2O
8-azaxanthosine + NH3
show the reaction diagram
adenine + H2O
allopurinol + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
cytidine + H2O
uridine + NH3
show the reaction diagram
-
-
-
?
guanosine + H2O
xanthosine + NH3
show the reaction diagram
additional information
?
-
-
no activity with adenine, adenosine, AMP, guanine, GMP, cytosine, cytidine, CMP, cyclic GMP, pterine, and tetrahydrofolate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2'-deoxyguanosine + H2O
2'-deoxyxanthosine + NH3
show the reaction diagram
-
-
-
-
?
adenine + H2O
allopurinol + NH3
show the reaction diagram
-
-
-
?
adenosine + H2O
inosine + NH3
show the reaction diagram
-
-
-
?
cytidine + H2O
uridine + NH3
show the reaction diagram
-
-
-
?
guanosine + H2O
xanthosine + NH3
show the reaction diagram
additional information
?
-
-
no activity with adenine, adenosine, AMP, guanine, GMP, cytosine, cytidine, CMP, cyclic GMP, pterine, and tetrahydrofolate
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-Amino-5-imidazole carboxamide ribonucleoside
-
-
p-chloromercuribenzoate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5761
2'-deoxyguanosine
-
at pH 8.0 and 40C
0.122
8-azaguanosine
-
-
0.062
deoxyguanosine
-
-
0.0098 - 0.264
guanosine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.611
2'-deoxyguanosine
Arabidopsis thaliana
-
at pH 8.0 and 40C
1.753
guanosine
Arabidopsis thaliana
-
at pH 8.0 and 40C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.061
2'-deoxyguanosine
Arabidopsis thaliana
-
at pH 8.0 and 40C
504
6.642
guanosine
Arabidopsis thaliana
-
at pH 8.0 and 40C
296
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
62.4 - 486
-
different brain areas, activity via nucleoside phosphorylase and guanine deaminase
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
rapidly decreased above 50C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000 - 20000
-
gel filtration
18000
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gel filtration
45000
-
x * 45000, enzyme-yellow fluorescent protein complex, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 45000, enzyme-yellow fluorescent protein complex, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 7 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana benthamiana
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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caffeine biosynthesis and purine metabolism