Information on EC 3.5.4.11 - pterin deaminase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.5.4.11
-
RECOMMENDED NAME
GeneOntology No.
pterin deaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 2-amino-4-hydroxypteridine + H2O = a 2,4-dihydroxypteridine + NH3
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolytic deamination
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
2-amino-4-hydroxypteridine aminohydrolase
The animal enzyme is specific for pterin, isoxanthopterin and tetrahydropterin.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-04-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Alcaligenes metalcaligenes
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-amino-3,5-dihydropteridine-4,6-dione + H2O
1,5-dihydropteridine-2,4,6(3H)-trione + NH3
show the reaction diagram
-
i.e. xanthopterin
-
-
?
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine + H2O
2,4-dihydroxy-6-hydroxymethyl-7,8-dihydropteridine + NH3
show the reaction diagram
2-amino-4-hydroxypteridine + H2O
2,4-dihydroxypteridine + NH3
show the reaction diagram
2-amino-4-oxo-3,4-dihydropteridine-6-carboxylic acid + H2O
2,4-dioxo-1,2,3,4-tetrahydropteridine-6-carboxylic acid + NH3
show the reaction diagram
2-amino-5,6,7,8-tetrahydropteridin-4(1H)-one + H2O
5,6,7,8-tetrahydropteridine-2,4(1H,3H)-dione + NH3
show the reaction diagram
-
the enzyme specifically deaminates pterin, isoxanthopterin and tetrahydropterin
-
-
?
2-amino-6-hydroxypteridin-4(3H)-one + H2O
6-hydroxypteridine-2,4(1H,3H)-dione + NH3
show the reaction diagram
-
i.e. 6-hydroxypterin
-
-
?
2-amino-6-methylpteridin-4(3H)-one + H2O
6-methylpteridine-2,4(1H,3H)-dione + NH3
show the reaction diagram
-
i.e. 6-methylpterin
-
-
?
2-amino-6-[(1R,2S)-1,2-dihydroxypropyl]pteridin-4(8H)-one + H2O
6-[(1R,2S)-1,2-dihydroxypropyl]pteridine-2,4(1H,3H)-dione + NH3
show the reaction diagram
-
i.e. biopterin
-
-
?
2-amino-6-[(2S)-2-hydroxypropanoyl]-7,8-dihydropteridin-4(1H)-one + H2O
6-[(2S)-2-hydroxypropanoyl]-7,8-dihydropteridine-2,4(1H,3H)-dione + NH3
show the reaction diagram
-
i.e. sepiapterin
-
-
?
2-amino-7-methylpteridin-4(3H)-one + H2O
7-methylpteridine-2,4(1H,3H)-dione + NH3
show the reaction diagram
-
i.e. 7-methylpterin
-
-
?
2-aminopteridin-4(1H)-one + H2O
(2S)-2-amino-2-hydroxy-2,3-dihydropteridin-4(1H)-one + NH3
show the reaction diagram
2-aminopteridin-4(1H)-one + H2O
2-hydroxypteridin-4(1H)-one + NH3
show the reaction diagram
2-aminopteridin-4(3H)-one + H2O
(2R)-2-amino-2-hydroxy-2,3-dihydropteridin-4(1H)-one + NH3
show the reaction diagram
2-aminopteridin-4(3H)-one + H2O
2-hydroxypteridin-4(3H)-one + NH3
show the reaction diagram
2-aminopteridine-4,7(1H,8H)-dione + H2O
7-hydroxypteridine-2,4(1H,3H)-dione + NH3
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-amino-4-hydroxypteridin-6-carboxylic acid
Alcaligenes metalcaligenes
-
-
4-chloromercuribenzoate
-
1 mM, 69% inhibition
8-Azaguanine
-
1 mM, 58% inhibition
Azaguanine
-
0.00145 mM, 57% inhibition
CN-
-
0.5 mM, 48% inhibition
KCN
-
10 mM, 12% inhibition
KF
Alcaligenes metalcaligenes
-
-
lumazine
-
0.00145 mM, 57% inhibition
NaF
Alcaligenes metalcaligenes
-
-
p-chloromercuribenzoate
Alcaligenes metalcaligenes
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
-
0.1-1 mM, about 25% increase in activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
2-Amino-4-hydroxypteridine
-
pH 6.5, 37C
0.14 - 1.3
2-amino-4-oxo-3,4-dihydropteridine-6-carboxylic acid
0.008
2-aminopteridine-4,7(1H,8H)-dione
pH 8.5, 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.01
2-amino-4-oxo-3,4-dihydropteridine-6-carboxylic acid
unidentified prokaryotic organism
P0CI72
pH 8.5, 30C
1.05
2-aminopteridine-4,7(1H,8H)-dione
unidentified prokaryotic organism
P0CI72
pH 8.5, 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.2
2-amino-4-oxo-3,4-dihydropteridine-6-carboxylic acid
unidentified prokaryotic organism
P0CI72
pH 8.5, 30C
19401
131.3
2-aminopteridine-4,7(1H,8H)-dione
unidentified prokaryotic organism
P0CI72
pH 8.5, 30C
41377
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.017
-
25C, pH 7.0
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 6.7
Alcaligenes metalcaligenes
-
although the reaction proceeds in the complete absence of phosphate, the rate of deamination is slightly faster in phosphate than in Tris buffer
7.3
-
substrate: 2-amino-4-oxo-3,4-dihydropteridine-6-carboxylic acid
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 8
-
pH 5.5: about 65% of maximal activity, pH 8.0: about 85% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest activity in 4-day-old larva
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
-
gel filtration
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
vapor diffusion method, diffraction quality crystals of the selenomethionine form of Sgx9339a are obtained in trigonal space group P3121 at room temperature
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing causes a severe loss of activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C or -80C, the activity is maintained for several months
-
4C, the activity decreases about 80% after standing overnight
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Strep-Tactin column chromatography, HisTrap column chromatography, and S200 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
Show AA Sequence (131 entries)
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