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Information on EC 3.5.3.9 - allantoate deiminase and Organism(s) Arabidopsis thaliana and UniProt Accession O49434

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IUBMB Comments
This enzyme is part of the ureide pathway, which permits certain organisms to recycle the nitrogen in purine compounds. This enzyme, which liberates ammonia from allantoate, is present in plants and bacteria. In plants it is localized in the endoplasmic reticulum. Requires manganese.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O49434
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
ataah, allantoate-degrading enzyme, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
allantoate amidohydrolase
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allantoate amidohydrolase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of amidines
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SYSTEMATIC NAME
IUBMB Comments
allantoate amidinohydrolase (decarboxylating)
This enzyme is part of the ureide pathway, which permits certain organisms to recycle the nitrogen in purine compounds. This enzyme, which liberates ammonia from allantoate, is present in plants and bacteria. In plants it is localized in the endoplasmic reticulum. Requires manganese.
CAS REGISTRY NUMBER
COMMENTARY hide
37289-13-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
show the reaction diagram
allantoate + H2O
ureidoglycine + NH3 + CO2
show the reaction diagram
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-
-
-
?
additional information
?
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substrate specificity, overview, no activity with N-alpha-carbamoyl-L-Asp, N-alpha-carbamoyl-L-Ala, and N-alpha-carbamoyl-Gly
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
allantoate + 2 H2O
(S)-ureidoglycolate + 2 NH3 + CO2
show the reaction diagram
allantoate degradation is required to recycle purine-ring nitrogen in plants
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-Asn
inhibition via chelating Mn2+, allantoate protects and reverses, competitive or mixed-type inhibition
L-Asp
inhibition via chelating Mn2+, allantoate protects and reverses
additional information
no inhibition by beta-mercaptoethanol, allantoin, urea, N-alpha-acetyl-L-Asn and N-alpha-acetyl-D-Asn, nitrate, and by phenylphosphorodiamidate
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.032
fluoride
Arabidopsis thaliana
pH 8.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
distribution, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
deletion mutant enzymes show symptoms similar to those of nitrogen deficiency: early flowering, reduced size at maturity, and decreased fertility
metabolism
ureide-degrading enzyme
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AAH_ARATH
525
0
56524
Swiss-Prot
Chloroplast (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
103000
gel filtration
53100
x * 53100, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 53100, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
an N-terminally truncated version of AAH-YFP lacking the first 71 amino acids exclusively localized to the cytosol
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant StrepII-tagged enzyme from Nicotiana tabacum leaf extracts to homogeneity by streptavidin affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
transient expression in Nicotiana tabacum as StrepII-tagged enzyme using the Agrobacterium tumefaciens transfection method, co-expression of YFP-tagged enzyme in Nicotiana tabacum plants with endoplasmic reticulum and peroxisomal markers
expression in Saccharomyces cerevisiae pDR196 and introduced in Saccharomyces cerevisiae mutant dal2 strain
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Todd, C.D.; Polacco, J.C.
AtAAH encodes a protein with allantoate amidohydrolase activity from Arabidopsis thaliana
Planta
223
1108-1113
2006
Arabidopsis thaliana
Manually annotated by BRENDA team
Werner, A.K.; Sparkes, I.A.; Romeis, T.; Witte, C.P.
Identification, biochemical characterization, and subcellular localization of allantoate amidohydrolases from Arabidopsis and soybean
Plant Physiol.
146
418-430
2008
Arabidopsis thaliana (O49434), Glycine max (A9GYV1), Glycine max
Manually annotated by BRENDA team
Takagi, H.; Watanabe, S.; Tanaka, S.; Matsuura, T.; Mori, I.C.; Hirayama, T.; Shimada, H.; Sakamoto, A.
Disruption of ureide degradation affects plant growth and development during and after transition from vegetative to reproductive stages
BMC Plant Biol.
18
287
2018
Arabidopsis thaliana (O49434)
Manually annotated by BRENDA team