Information on EC 3.5.3.26 - (S)-ureidoglycine aminohydrolase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.5.3.26
-
RECOMMENDED NAME
GeneOntology No.
(S)-ureidoglycine aminohydrolase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-2-ureidoglycine + H2O = (S)-ureidoglycolate + NH3
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
allantoin degradation to glyoxylate II
-
-
allantoin degradation to ureidoglycolate II (ammonia producing)
-
-
Microbial metabolism in diverse environments
-
-
Purine metabolism
-
-
allantoin degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
(S)-ureidoglycine amidinohydrolase
Binds Mn2+. This enzyme, found in plants and bacteria, is part of the ureide pathway, which enables the recycling of the nitrogen in purine compounds. In plants it is localized in the endoplasmic reticulum.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-2-ureidoglycine + H2O
(S)-ureidoglycolate + NH3
show the reaction diagram
-
-
-
-
?
(S)-ureidoglycine + H2O
(S)-ureidoglycolate + NH3
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-2-ureidoglycine + H2O
(S)-ureidoglycolate + NH3
show the reaction diagram
-
-
-
-
?
(S)-ureidoglycine + H2O
(S)-ureidoglycolate + NH3
show the reaction diagram
P75713
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.77 - 3.46
(S)-2-ureidoglycine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
73 - 761
(S)-2-ureidoglycine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
32 - 429
(S)-2-ureidoglycine
176138
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30200
-
8 * 30200, SDS-PAGE
200000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homooctamer
-
8 * 30200, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
seleno-L-methionine-labelled enzyme, hanging drop vapor diffusion method, using either 0.1 M phosphate citrate, pH 4.2, 10% (w/v) PEG 3000, 0.2 M NaCl or 0.1 M HEPES, pH 7.5, 7% (w/v) PEG 8000, 8% (v/v) ethylene glycol
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
immobilized metal affinity column chromatography and Superdex 200 gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli B834 and BL21 (DE3) cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E235A
-
inactive
E235Q
-
inactive
H237A
-
inactive
H241A
-
inactive
K291A
-
inactive
K291R
-
the mutant shows reduced activity compared to the wild type
Q275A
-
inactive
Y252F
-
the mutant shows reduced activity compared to the wild type
Y287A
-
inactive
Y287F
-
inactive