Information on EC 3.5.3.24 - N1-aminopropylagmatine ureohydrolase

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The expected taxonomic range for this enzyme is: Archaea, Bacteria

EC NUMBER
COMMENTARY hide
3.5.3.24
-
RECOMMENDED NAME
GeneOntology No.
N1-aminopropylagmatine ureohydrolase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N1-aminopropylagmatine + H2O = spermidine + urea
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
spermidine biosynthesis III
-
-
polyamine pathway
-
-
SYSTEMATIC NAME
IUBMB Comments
N1-aminopropylagmatine amidinohydrolase
The enzyme, which has been characterized from the hyperthermophilic archaeon Pyrococcus kodakarensis and the thermophilic Gram-negative bacterium Thermus thermophilus, is involved in the biosynthesis of spermidine.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
a disruption mutant accumulates N1-aminopropylagmatine
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
agmatine + H2O
putrescine + urea
show the reaction diagram
43fold higher kcat/Km value for N1-aminopropylagmatine than for agmatine
-
-
?
N1-aminopropylagmatine + H2O
spermidine + urea
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N1-aminopropylagmatine + H2O
spermidine + urea
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.486
agmatine
pH 7.5, 70°C
0.00642
N1-aminopropylagmatine
pH 7.5, 70°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00186
agmatine
Thermococcus kodakarensis
Q5JI38
pH 7.5, 70°C
0.001
N1-aminopropylagmatine
Thermococcus kodakarensis
Q5JI38
pH 7.5, 70°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00387
agmatine
Thermococcus kodakarensis
Q5JI38
pH 7.5, 70°C
505
0.165
N1-aminopropylagmatine
Thermococcus kodakarensis
Q5JI38
pH 7.5, 70°C
15292
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32500
x * 32500, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 32500, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90
enzyme is not fully unfolded
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
overexpression in Escherichia coli