Information on EC 3.5.3.21 - methylenediurea deaminase

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The expected taxonomic range for this enzyme is: Ochrobactrum anthropi

EC NUMBER
COMMENTARY hide
3.5.3.21
-
RECOMMENDED NAME
GeneOntology No.
methylenediurea deaminase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
methylenediurea + 2 H2O = N-(hydroxymethyl)urea + 2 NH3 + CO2
show the reaction diagram
The product N-(carboxyaminomethyl)urea decomposes spontaneously into CO2, NH3, formaldehyde and urea according to the reactions: (1) N-(carboxyaminomethyl)urea = N-(aminomethyl)urea + CO2 (2) N-(aminomethyl)urea + H2O = N-(hydroxymethyl)urea + NH3 (3) N-(hydroxymethyl)urea = urea + formaldehyde
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-
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methylenediurea + H2O = N-(carboxyaminomethyl)urea + NH3
show the reaction diagram
(1a)
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-
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N-(aminomethyl)urea + H2O = N-(hydroxymethyl)urea + NH3
show the reaction diagram
spontaneous
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-
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N-(carboxyaminomethyl)urea = N-(aminomethyl)urea + CO2
show the reaction diagram
spontaneous
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amidines
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
NIL
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-
SYSTEMATIC NAME
IUBMB Comments
methylenediurea aminohydrolase
Methylenediurea is hydrolysed and decarboxylated to give an aminated methylurea, which then spontaneously hydrolyses to hydroxymethylurea. The enzyme from Ochrobactrum anthropi also hydrolyses dimethylenetriurea and trimethylenetetraurea as well as ureidoglycolate, which is hydrolysed to urea and glyoxylate, and allantoate, which is hydrolysed to ureidoglycolate, ammonia and carbon dioxide.
CAS REGISTRY NUMBER
COMMENTARY hide
205830-62-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain UF4
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Allantoate + H2O
Glyoxylate + urea + ammonium
show the reaction diagram
dimethylenetriurea + H2O
ammonium + formaldehyde + urea + CO2
show the reaction diagram
dimethylenetriurea + H2O
urea + NH3 + formaldehyde + CO2
show the reaction diagram
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-
-
-
?
methylenediurea + H2O
ammonium + formaldehyde + urea + CO2
show the reaction diagram
methylenediurea + H2O
urea + NH3 + formaldehyde + CO2
show the reaction diagram
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-
-
-
?
trimethylenetetraurea + H2O
ammonium + formaldehyde + urea + CO2
show the reaction diagram
Ureidoglycolate + H2O
Glyoxylate + urea
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Acetohydroxamate
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0004
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with NH4Cl as nitrogen source
0.97
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with methylenurea as nitrogen source
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
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pH 7: about 25% of maximal activity, pH 9.5: about 70% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 70
-
30°C: about 45% of maximal activity, 70°C: about 30% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
4 * 38000, SDS-PAGE
125000
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native PAGE
140000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 11
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60 min, stable
32167
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
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30 min, stable up to
80
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30 min, complete loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE