Information on EC 3.5.3.20 - diguanidinobutanase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
3.5.3.20
-
RECOMMENDED NAME
GeneOntology No.
diguanidinobutanase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1,4-diguanidinobutane + H2O = agmatine + urea
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amidines
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
1,4-diguanidinobutane amidinohydrolase
Other diguanidinoalkanes with 3 to 10 methylene groups can also act, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
125268-65-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-diguanidinobutane + H2O
agmatine + urea
show the reaction diagram
1-amino-5-guanidinopentane + H2O
1,5-diaminopentane + urea
show the reaction diagram
-
-
-
-
?
1-amino-6-guanidinohexane + H2O
1,6-diaminohexane + urea
show the reaction diagram
-
-
-
-
?
1-amino-7-guanidinoheptane + H2O
1,7-diaminoheptane + urea
show the reaction diagram
-
-
-
-
?
agmatine + H2O
putrescine + urea
show the reaction diagram
diguanidinoalkanoate + H2O
aminoalkylguanidine + urea
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,4-diguanidinobutane + H2O
agmatine + urea
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
inert
Co2+
-
required for full activity
Mg2+
-
inert
Ni2+
-
required for full activity
Zn2+
-
inert
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-Diaminodecane
-
41% inhibition at concentration of 3.0 mM
1,7-Diaminoheptane
-
22% inhibition at concentration of 5.0 mM
1,8-diaminooctane
-
34% inhibition at concentration of 3.0 mM
1,9-diaminononane
-
38% inhibition at concentration of 3.0 mM
1-amino-5-guanidinopentane
-
29% inhibition at concentration of 5.0 mM
5-Guanidinovalerate
-
10% inhibition at concentration of 5.0 mM
agmatine
-
28% inhibition at concentration of 5.0 mM
DTNB
-
11% inhibition at concentration of 5.0 mM
N-carbamoylagmatine
-
62% inhibition at concentration of 5.0 mM
p-chloromercuribenzoate
-
complete loss of activity at concentration of 5.0 mM, 30% reactivation by incubation with 10.0 mM 2-mercaptoethanol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.91
1,3-diguanidinopropane
-
-
-
0.65 - 0.7
1,4-Diguanidinobutane
0.5
1,5-Diguanidinopentane
-
-
0.33
1,6-Diguanidinohexane
-
-
0.24
1,7-Diguanidinoheptane
-
-
0.23
1,8-Diguanidinooctane
-
-
2.5
1-amino-5-guanidinopentane
-
-
1.3
1-amino-6-guanidinohexane
-
-
1
1-amino-7-guanidinoheptane
-
-
3
agmatine
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1330
1,4-Diguanidinobutane
Pseudomonas putida
-
per subunit
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 11
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
170000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4*38000 Da, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
most stable
209443
7 - 8
-
stable in potassium phosphate
209444
7 - 9
-
stable in TRIS-HCl
209444
10
-
stable in piperazine hydrochloride
209444
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
no loss of activity within 2 h in presence of 10 mM EDTA
50
-
unstable on heating for 15 min
60
-
complete inactivation in presence and absence of Mn2+
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
pH 7.0-8.0
-
pH 7.0-9.0
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
full purification
-
partial purification
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
preparation of pure agmatine sulfate