Information on EC 3.5.3.14 - amidinoaspartase

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The expected taxonomic range for this enzyme is: Pseudomonas

EC NUMBER
COMMENTARY hide
3.5.3.14
-
RECOMMENDED NAME
GeneOntology No.
amidinoaspartase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-amidino-L-aspartate + H2O = L-aspartate + urea
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
amidine hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
N-amidino-L-aspartate amidinohydrolase
Also acts slowly on N-amidino-L-glutamate.
CAS REGISTRY NUMBER
COMMENTARY hide
37325-60-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Pseudomonas chlororaphis
-
-
-
Manually annotated by BRENDA team
no activity in Pseudomonas chlororaphis ATCC 9446
-
-
-
Manually annotated by BRENDA team
fluorescent Pseudomonas
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
guanidinoglutaric acid + H2O
?
show the reaction diagram
-
-
-
-
?
N-amidino-L-aspartic acid
L-aspartic acid + urea
show the reaction diagram
N-amidino-L-aspartic acid + H2O
L-aspartic acid + urea
show the reaction diagram
N-amidino-L-glutamate + H2O
L-glutamic acid + urea
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-amidino-L-aspartic acid
L-aspartic acid + urea
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
C-32, specifically activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
aspartic acid
-
competitive inhibition
Urea
-
competitive inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0094 - 20
N-amidino-L-aspartate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9 - 10
-
C-32
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39800
-
5 * 39800, C-32, SDS-PAGE
240000
-
C-32, gel filtration
300000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentamer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 50
-
incubation at 40°C results in rapid activation, prolonged incubation causes irreversible loss of activity, incubation at 50°C 90% of the initial enzyme activity lost within 10 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, stable for several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
test for N-amidino-L-aspartate in the urine of uremic patients