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Information on EC 3.5.2.3 - dihydroorotase and Organism(s) Mesocricetus auratus and UniProt Accession P08955

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.3 dihydroorotase
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This record set is specific for:
Mesocricetus auratus
UNIPROT: P08955 not found.
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Word Map
The taxonomic range for the selected organisms is: Mesocricetus auratus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
dihydroorotase, dho, dhoase, dihydroorotase domain, hudhoase, type i dhoase, human dhoase domain, type ii dho, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carbamoylaspartic dehydrase
-
-
-
-
DHOase
dihydroorotate dehydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of cyclic amides
-
-
-
-
formation of cyclic amides
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
(S)-dihydroorotate amidohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9024-93-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-dihydroorotate + H2O
N-carbamoyl-L-aspartate
show the reaction diagram
dexrazoxane + H2O
ADR-925
show the reaction diagram
-
i.e. ICRF187 or (+)-1,2-bis(3,5-dioxopiperazin-1-yl)propane, cardioprotective agent
i.e. N,N’-[(1S)-1-methyl-1,2-ethanediyl]bis[N-(2-amino-2-oxoethyl)glycine], biologically active form
?
N-carbamoyl-L-aspartate
L-dihydroorotate + H2O
show the reaction diagram
-
-
-
-
r
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxo-1,2,3,6-tetrahydropyrimidine-4,6-dicarboxylic acid
-
competitive inhibitor
5-aminoorotate
-
1 mM, 90% inhibition
Furosemide
-
1 mM, 80% inhibition
additional information
-
not inhibitory: 4-chlorobenzenesulfonamide
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
(S)-dihydroorotate
-
pH 7.4, 15°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0045
2-oxo-1,2,3,6-tetrahydropyrimidine-4,6-dicarboxylic acid
-
at 37°C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 45
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PYR1_MESAU
2225
0
243128
Swiss-Prot
other Location (Reliability: 3)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
-
temperature-dependent conversion to homotetramer, crystallization data
homotetramer
-
temperature-dependent conversion to homodimer, crystallization data
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
at 50°C the enzyme remains stable for 10 min
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
N-linked butylamine-agarose column chromatography and DEAE-Sephacel column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli SO1263 cells
-
expressed in Escherichia coli strain SO1263
-
overexpression in Escherichia coli strain SØ1263/pyrC-
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
activation of the cardioprotective agent dexrazoxane by the enzyme in heart, mechanism
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Maher, M.J.; Huang, D.T.; Guss, J.M.; Collyer, C.A.; Christopherson, R.I.
Crystallization of hamster dihydroorotase: involvement of a disulfide-linked tetrameric form
Acta Crystallogr. Sect. D
59
381-384
2003
Mesocricetus auratus
Manually annotated by BRENDA team
Schroeder, P.E.; Davidson, J.N.; Hasinoff, B.B.
Dihydroorotase catalyzes the ring opening of the hydrolysis intermediates of the cardioprotective drug dexrazoxane (ICRF-187)
Drug Metab. Dispos.
30
1431-1435
2002
Mesocricetus auratus
Manually annotated by BRENDA team
Anderson, M.A.; Cleland, W.W.; Huang, D.T.; Chan, C.; Shojaei, M.; Christopherson, R.I.
13C and 15N isotope effects for conversion of L-dihydroorotate to N-carbamyl-L-aspartate using dihydroorotase from hamster and Bacillus caldolyticus
Biochemistry
45
7132-7139
2006
[Bacillus] caldolyticus, Mesocricetus auratus
Manually annotated by BRENDA team
Huang, D.T.; Kaplan, J.; Menz, R.I.; Katis, V.L.; Wake, R.G.; Zhao, F.; Wolfenden, R.; Christopherson, R.I.
Thermodynamic analysis of catalysis by the dihydroorotases from hamster and Bacillus caldolyticus, as compared with the uncatalyzed reaction
Biochemistry
45
8275-8283
2006
[Bacillus] caldolyticus, Cricetinae, Mesocricetus auratus
Manually annotated by BRENDA team
Lee, M.; Chan, C.W.; Mitchell Guss, J.; Christopherson, R.I.; Maher, M.J.
Dihydroorotase from Escherichia coli: loop movement and cooperativity between subunits
J. Mol. Biol.
348
523-533
2005
Arabidopsis thaliana (O04904), Escherichia coli (P05020), Escherichia coli, Mesocricetus auratus (P08955), Saccharomyces cerevisiae (P20051), Homo sapiens (P27708), Helicobacter pylori (P56465), Plasmodium falciparum (Q8IKA9)
Manually annotated by BRENDA team
Robles Lopez, S.M.; Hortua Triana, M.A.; Zimmermann, B.H.
Cloning and preliminary characterization of the dihydroorotase from Toxoplasma gondii
Mol. Biochem. Parasitol.
148
93-98
2006
Saccharomyces cerevisiae, Mycobacterium leprae, Plasmodium berghei (A0A509AX79), Arabidopsis thaliana (O04904), Trypanosoma cruzi (O76138), Escherichia coli (P05020), Mesocricetus auratus (P08955), Homo sapiens (P27708), Ustilago maydis (P31301), Toxoplasma gondii (Q8MXA5), Toxoplasma gondii
Manually annotated by BRENDA team