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Information on EC 3.5.2.2 - dihydropyrimidinase and Organism(s) Dictyostelium discoideum and UniProt Accession Q55DL0

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EC Tree
     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.2 In cyclic amides
                3.5.2.2 dihydropyrimidinase
IUBMB Comments
Also acts on dihydrothymine and hydantoin.
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This record set is specific for:
Dictyostelium discoideum
UNIPROT: Q55DL0
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Word Map
The taxonomic range for the selected organisms is: Dictyostelium discoideum
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
dhp, dihydropyrimidine dehydrogenase, dihydropyrimidinase, hydantoinase, d-hydantoinase, dhp-1, dhpase, dihydropyrimidine amidohydrolase, dhp-2, padhpase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dihydropyrimidine amidohydrolase
-
D-hydantoinase
-
-
-
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DHP
-
-
-
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Dihydropyrimidinase
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-
-
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dihydropyrimidine amidohydrolase
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hydantoin peptidase
-
-
-
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hydantoinase
-
-
-
-
hydropyrimidine hydrase
-
-
-
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pyrimidine hydrase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic acid amide hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
5,6-dihydropyrimidine amidohydrolase
Also acts on dihydrothymine and hydantoin.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-74-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,6-dihydrothymine + H2O
3-(carbamoylamino)-2-methylpropanoic acid
show the reaction diagram
-
-
-
?
5,6-dihydrouracil + H2O
N-carbamoyl-beta-alanine
show the reaction diagram
-
-
-
?
5,6-dihydrouracil + H2O
N-carbamyl-beta-alanine
show the reaction diagram
-
-
-
?
N-carbamoyl-beta-alanine + H2O
?
show the reaction diagram
-
-
-
?
5,6-dihydrothymine + H2O
?
show the reaction diagram
-
-
-
-
?
5,6-dihydrouracil + H2O
3-ureidopropionate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
does not hydrolyze hydantoins
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
zinc-metalloenzyme
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DPYS_DICDI
503
0
56032
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
homotetramer
-
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
with 100 mM Bistris, pH 6.5, 20% (w/v) PEG 3350, and 100 mM trisodium citrate
X-ray structure at at 2.05 A resolution
by vapour-diffusion method with sitting drops
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, phosphate buffer, pH 7.0, 10% (v/v) glycerol
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gel filtration, metal-affinity chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression as His8-tagged fusion protein in Escherichia coli BL21
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lohkamp, B.; Andersen, B.; Piskur, J.; Dobritzsch, D.
Purification, crystallization and X-ray diffraction analysis of dihydropyrimidinase from Dictyostelium discoideum
Acta Crystallogr. Sect. F
62
36-38
2005
Dictyostelium discoideum
Manually annotated by BRENDA team
Rawls, J.M.
Analysis of pyrimidine catabolism in Drosophila melanogaster using epistatic interactions with mutations of pyrimidine biosynthesis and beta-alanine metabolism
Genetics
172
1665-1674
2006
Caenorhabditis elegans (Q18677), Caenorhabditis elegans (Q21773), Drosophila melanogaster (Q2XST3), Drosophila melanogaster (Q8IPQ2), Drosophila melanogaster, Dictyostelium discoideum (Q55DL0), Mus musculus (Q9EQF5), Arabidopsis thaliana (Q9FMP3), Pseudomonas aeruginosa (Q9I676), Lachancea kluyveri (Q9P903), Drosophila melanogaster EP(3)3238 (Q2XST3), Drosophila melanogaster EP(3)3238 (Q8IPQ2)
Manually annotated by BRENDA team
Lohkamp, B.; Andersen, B.; Piskur, J.; Dobritzsch, D.
The crystal structures of dihydropyrimidinases reaffirm the close relationship between cyclic amidohydrolases and explain their substrate specificity
J. Biol. Chem.
281
13762-13776
2006
Dictyostelium discoideum (Q55DL0), Dictyostelium discoideum, Lachancea kluyveri (Q9P903), Lachancea kluyveri
Manually annotated by BRENDA team
Schnackerz, K.D.; Dobritzsch, D.
Amidohydrolases of the reductive pyrimidine catabolic pathway: Purification, characterization, structure, reaction mechanisms and enzyme deficiency
Biochim. Biophys. Acta
1784
431-444
2008
Bos taurus, Dictyostelium discoideum, Homo sapiens, Rattus norvegicus, Lachancea kluyveri
Manually annotated by BRENDA team