Information on EC 3.5.2.18 - enamidase

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The expected taxonomic range for this enzyme is: Eubacterium barkeri

EC NUMBER
COMMENTARY hide
3.5.2.18
-
RECOMMENDED NAME
GeneOntology No.
enamidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
6-oxo-1,4,5,6-tetrahydronicotinate + 2 H2O = 2-formylglutarate + NH3
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of C-N bond
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
-
-
Nicotinate and nicotinamide metabolism
-
-
nicotinate degradation III
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-
SYSTEMATIC NAME
IUBMB Comments
6-oxo-1,4,5,6-tetrahydronicotinate amidohydrolase
Contains iron and Zn2+. Forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri. Other enzymes involved in this pathway are EC 1.17.1.5 (nicotinate dehydrogenase), EC 1.3.7.1 (6-hydroxynicotinate reductase), EC 1.1.1.291 (2-hydroxymethylglutarate dehydrogenase), EC 5.4.99.4 (2-methyleneglutarate mutase), EC 5.3.3.6 (methylitaconate Delta-isomerase), EC 4.2.1.85 (dimethylmaleate hydratase) and EC 4.1.3.32 (2,3-dimethylmalate lyase).
CAS REGISTRY NUMBER
COMMENTARY hide
911980-63-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4,5,6-tetrahydro-6-oxonicotinate + H2O
2-(enamine)glutarate + NH3
show the reaction diagram
-
-
-
?
2-(enamine)glutarate + H2O
(S)-2-formylglutarate + NH3
show the reaction diagram
-
-
-
?
6-oxo-1,4,5,6-tetrahydronicotinate + H2O
2-formylglutarate + NH3
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-oxo-1,4,5,6-tetrahydronicotinate + H2O
2-formylglutarate + NH3
show the reaction diagram
-
the enzyme forms part of the nicotinate-fermentation catabolism pathway in Eubacterium barkeri
-
-
r
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe
-
contains 1.0 Fe per subunit. The Fe/Zn binuclear metal center of enamidase catalyzes amide hydrolysis of 6-oxo-1,4,5,6-tetrahydronicotinate, hydration and ammonia elimination
Zn
-
contains about 0.6 Zn per subunit
additional information
-
the enzyme contains the typical metal binding His-X-His pattern in the N-terminal part
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39793
-
4 * 39793, MALDI-TOF MS
40000
-
4 * 40000, SDS-PAGE
178000
size-exclusion chromatography
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli strain BL 21