Information on EC 3.5.2.1 - barbiturase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.5.2.1
-
RECOMMENDED NAME
GeneOntology No.
barbiturase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
barbiturate + H2O = 3-oxo-3-ureidopropanoate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Pyrimidine metabolism
-
-
uracil degradation II (oxidative)
-
-
SYSTEMATIC NAME
IUBMB Comments
barbiturate amidohydrolase (3-oxo-3-ureidopropanoate-forming)
Contains zinc and is specific for barbiturate as substrate [3]. Forms part of the oxidative pyrimidine-degrading pathway in some microorganisms, along with EC 1.17.99.4 (uracil/thymine dehydrogenase) and EC 3.5.1.95 (N-malonylurea hydrolase). It was previously thought that the end-products of the reaction were malonate and urea but this has since been disproved [2]. May be involved in the regulation of pyrimidine metabolism, along with EC 2.4.2.9, uracil phosphoribosyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-16-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
barbiturate + H2O
?
show the reaction diagram
-
induced by uracil, enzyme is involved in the oxidative catabolism of uracil
-
-
-
barbiturate + H2O
malonate + urea
show the reaction diagram
barbiturate + H2O
ureidomalonic acid
show the reaction diagram
strict specificity
-
?
ureidomalonic acid
barbiturate + H2O
show the reaction diagram
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
barbiturate + H2O
?
show the reaction diagram
-
induced by uracil, enzyme is involved in the oxidative catabolism of uracil
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
4.4 mol per mol of enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
2 mM, 30% inhibition
5,5'-dithio-bis-(2-nitrobenzoic acid)
2 mM, 50% inhibition
8-hydroxyquinoline
2 mM, 60% inhibition
alloxan
competitive
Cd2+
2 mM, 60% inhibition
Co2+
2 mM, 50% inhibition
Cu2+
2 mM, complete inhibition
cyanuric acid
competitive
dihydro-L-orotate
competitive
diisopropyl phosphofluoridate
2 mM, 90% inhibition
EDTA
2 mM, 30% inhibition
Hg2+
2 mM, complete inhibition
N-bromosuccinimide
2 mM, 60% inhibition
N-ethylmaleimide
2 mM, complete inhibition
Ni2+
2 mM, 80% inhibition
o-phenanthroline
2 mM, 80% inhibition
p-chloromercuribenzoate
2 mM, complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1 - 3.37
Barbiturate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.7
alloxan
pH 8.0, 30C
0.42
cyanuric acid
pH 8.0, 30C
4.5
dihydro-L-orotate
pH 8.0, 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
loss of activity above
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9.5
-
pH 7.5: about 70% of maximal activity, pH 9.5: about 45% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
4 * 45000, SDS-PAGE
172000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
4 * 45000, SDS-PAGE
additional information
partial amino acid sequence analysis
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
30 min, complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, 10% (v/v) ethylene glykol, stable up to 3 months
4C, cell-free extract, loss of 70% of activity during 2 weeks
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE