Information on EC 3.5.1.94 - gamma-glutamyl-gamma-aminobutyrate hydrolase

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The expected taxonomic range for this enzyme is: Escherichia coli K-12

EC NUMBER
COMMENTARY hide
3.5.1.94
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RECOMMENDED NAME
GeneOntology No.
gamma-glutamyl-gamma-aminobutyrate hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-(gamma-L-glutamylamino)butanoate + H2O = 4-aminobutanoate + L-glutamate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of amide bond
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
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Metabolic pathways
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putrescine degradation II
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polyamine pathway
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SYSTEMATIC NAME
IUBMB Comments
4-(glutamylamino)butanoate amidohydrolase
Forms part of a putrescine-utilizing pathway in Escherichia coli, in which it has been hypothesized that putrescine is first glutamylated to form gamma-glutamylputrescine, which is oxidized to 4-(gamma-glutamylamino)butanal and then to 4-(gamma-glutamylamino)butanoate. The enzyme can also catalyse the reactions of EC 3.5.1.35 (D-glutaminase) and EC 3.5.1.65 (theanine hydrolase).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-(gamma-glutamylamino)butanoate + H2O
4-aminobutanoate + L-glutamate
show the reaction diagram
enzyme is involved in the gamma-glutamyl-gamma-aminobutyrate hydrolase pathway for the degradation of putrescine to succinate semialdehyde. PuuD is essential for growth on putrescine as a sole source of nitrogen. Expression of the enzyme is induced by putrescine and O2. Addition of succinate and NH4Cl to the medium reduces the expression of PuuD
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?
gamma-(glutamylamino)butanoate + H2O
gamma-aminobutanoate + L-glutamate
show the reaction diagram
Cys114 is the active center of PuuD
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-(gamma-glutamylamino)butanoate + H2O
4-aminobutanoate + L-glutamate
show the reaction diagram
P76038
enzyme is involved in the gamma-glutamyl-gamma-aminobutyrate hydrolase pathway for the degradation of putrescine to succinate semialdehyde. PuuD is essential for growth on putrescine as a sole source of nitrogen. Expression of the enzyme is induced by putrescine and O2. Addition of succinate and NH4Cl to the medium reduces the expression of PuuD
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-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28500
2 * 28500, calcaulation from amino-acid sequence
57000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 28500, calcaulation from amino-acid sequence
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stabilization of PuuD activity by beta-mercaptoethanol
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (465 entries)
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