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F177H
beta-subunit mutant, 17.6% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
F177P
beta-subunit mutant, only partial intramolecular cleavage and no intermolecular cleavage in posttranslational modification, no activity with glutaryl-7-aminocephalosporanic acid
F177T
beta-subunit mutant, 4.3% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate. Only partial intermolecular cleavage in posttranslational modification
Q50L
beta-subunit mutant, 9.9% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Q50M
beta-subunit mutant, 7.5% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Q50R
beta-subunit mutant, 2.1% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Q50T
beta-subunit mutant, 72.5% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Q50Y
beta-subunit mutant, 15.7% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
R57C
beta-subunit mutant, no intermolecular cleavage in posttranslational modification, no activity with glutaryl-7-aminocephalosporanic acid
R57I
beta-subunit mutant, no intermolecular cleavage in posttranslational modification, no activity with glutaryl-7-aminocephalosporanic acid
R57K
beta-subunit mutant, 0.3% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, only partial intermolecular cleavage in posttranslational modification
S1A
beta-subunit mutant, no processing takes place, no activity with glutaryl-7-aminocephalosporanic acid
S1C
beta-subunit mutant, no intermolecular cleavage in posttranslational modification, no activity with glutaryl-7-aminocephalosporanic acid
Y149C
alpha-subunit mutant, 16.4% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149G
alpha-subunit mutant, 0.7% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149L
alpha-subunit mutant, 6.3% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149N
alpha-subunit mutant, 30.8% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149P
alpha-subunit mutant, 1.1% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149R
alpha-subunit mutant, 13.6% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y33F
beta-subunit mutant, 65.4% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y33I
beta-subunit mutant, 9.3% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate only partial intermolecular cleavage in posttranslational modification
Y33S
beta-subunit mutant, no intermolecular cleavage in posttranslational modification, no activity with glutaryl-7-aminocephalosporanic acid
F177H
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 17.6% of the wild-type activity, no activity with cephalosporin C
F177P
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
F177T
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 4.3% of the wild-type activity, no activity with cephalosporin C
L24F
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 25.9% of the wild-type activity
L24G
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 27% of the wild-type activity, no activity with cephalosporin C
L24K
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 1.1% of the wild-type activity, no activity with cephalosporin C
L24R
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 87.7% of the wild-type activity
L24W
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 11.3% of the wild-type activity, activity with cephalosporin C is 98.8% of wild-type activity
Q50L
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 9.9% of the wild-type activity, no activity with cephalosporin C
Q50M
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 7.5% of the wild-type activity, activity with cephalosporin C is 180% of wild-type activity
Q50M/F58A
-
beta-subunit mutation, activity with cephalosporin C is 180% of wild-type activity
Q50M/F58D
-
beta-subunit mutation, activity with cephalosporin C is 130% of wild-type activity
Q50M/F58G
-
beta-subunit mutation, activity with cephalosporin C is 290% of wild-type activity
Q50M/F58H
-
beta-subunit mutation, activity with cephalosporin C is 160% of wild-type activity
Q50M/F58I
-
beta-subunit mutation, activity with cephalosporin C is 150% of wild-type activity
Q50M/F58L
-
beta-subunit mutation, activity with cephalosporin C is 120% of wild-type activity
Q50M/F58P
-
beta-subunit mutation, activity with cephalosporin C is 240% of wild-type activity
Q50M/F58S
-
beta-subunit mutation, activity with cephalosporin C is 260% of wild-type activity
Q50M/F58T
-
beta-subunit mutation, activity with cephalosporin C is 230% of wild-type activity
Q50M/Y149F
-
Q50M mutation in beta-subunit, Y149F mutation in alpha-subunit, activity with cephalosporin C is 157% of wild-type activity
Q50M/Y149K
-
Q50M mutation in beta-subunit, Y149K mutation in alpha-subunit, activity with cephalosporin C is 600% of wild-type activity
Q50M/Y149K/F177G
-
beta-subunit mutation, activity with cephalosporin C is 787% of wild-type activity
Q50M/Y149K/F177P
-
beta-subunit mutation, activity with cephalosporin C is 301% of wild-type activity
Q50M/Y149K/F177S
-
beta-subunit mutation, activity with cephalosporin C is 456% of wild-type activity
Q50M/Y149K/M145A
-
Q50M and Y149K are mutation in the beta-subunit, M145A is a mutation in the alpha-subunit, activity with cephalosporin C is 335% of wild-type activity
Q50M/Y149K/M145L
-
Q50M and Y149K are mutations in the beta-subunit, M145L is a mutation in the alpha-subunit, activity with cephalosporin C is 704% of wild-type activity
Q50M/Y149K/M145P
-
Q50M and Y149K are mutations in the beta-subunit, M145P is a mutation in the alpha-subunit, activity with cephalosporin C is 442% of wild-type activity
Q50M/Y149K/M145T
-
Q50M and Y149K are mutation in the beta-subunit, M145T is a mutation in the alpha-subunit, activity with cephalosporin C is 433% of wild-type activity
Q50M/Y149K/Y33N
-
beta-subunit mutation, activity with cephalosporin C is 460% of wild-type activity
Q50M/Y149K/Y33S
-
beta-subunit mutation, activity with cephalosporin C is 568% of wild-type activity
Q50M/Y149K/Y33T
-
beta-subunit mutation, activity with cephalosporin C is 424% of wild-type activity
Q50M/Y33D
-
beta-subunit mutant, activity with cephalosporin C is 450% of wild-type activity
Q50M/Y33G
-
beta-subunit mutant, activity with cephalosporin C is 70% of wild-type activity
Q50M/Y33H
-
beta-subunit mutant, activity with cephalosporin C is 101% of wild-type activity
Q50M/Y33L
-
beta-subunit mutant, activity with cephalosporin C is 202% of wild-type activity
Q50M/Y33N
-
beta-subunit mutant, activity with cephalosporin C is 350% of wild-type activity
Q50M/Y33P
-
beta-subunit mutant, activity with cephalosporin C is 99% of wild-type activity
Q50M/Y33S
-
beta-subunit mutant, activity with cephalosporin C is 60% of wild-type activity
Q50M/Y33T
-
beta-subunit mutant, activity with cephalosporin C is 109% of wild-type activity
Q50M/Y33V
-
beta-subunit mutant, activity with cephalosporin C is 135% of wild-type activity
Q50R
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 2.1% of the wild-type activity, no activity with cephalosporin C
Q50T
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 72.5% of the wild-type activity, no activity with cephalosporin C
Q50Y
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 15.7% of the wild-type activity, no activity with cephalosporin C
R155G
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 88% of the wild-type activity
R57C
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
R57I
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
R57K
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 0.3% of the wild-type activity, no activity with cephalosporin C
R57S
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
S152G
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 75.4% of the wild-type activity, activity with cephalosporin C is 38.6% of wild-type activity
S152H
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 63.7% of the wild-type activity, activity with cephalosporin C is 11.9% of wild-type activity
S152N
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 78% of the wild-type activity, activity with cephalosporin C is 24.8% of wild-type activity
S152P
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 76.2% of the wild-type activity, activity with cephalosporin C is 60.4% of wild-type activity
S152T
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 81.4% of the wild-type activity, activity with cephalosporin C is 34.7% of wild-type activity
S1A
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
S1betaC
-
no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
V70G
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 32.1% of the wild-type activity
V70S
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 28.8% of the wild-type activity, no activity with cephalosporin C
Y149C
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 16.4% of the wild-type activity, activity with cephalosporin C is 24% of wild-type activity
Y149G
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 0.7% of the wild-type activity, no activity with cephalosporin C
Y149L
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 6.3% of the wild-type activity, activity with cephalosporin C is 48% of wild-type activity
Y149N
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 30.8% of the wild-type activity, activity with cephalosporin C is 48% of wild-type activity
Y149P
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 1.1% of the wild-type activity, no activity with cephalosporin C
Y149R
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 13.6% of the wild-type activity, no activity with cephalosporin C
Y33F
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 65.4% of the wild-type activity, no activity with cephalosporin C
Y33I
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 9.3% of the wild-type activity, activity with cephalosporin C is 90% of wild-type activity
Y33S
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 120% of the wild-type activity
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Oh, B.; Kim, M.; Yoon, J.; Chung, K.; Shin, Y.; Lee, D.; Kim, Y.
Deacylation activity of cephalosporin acylase to cephalosporin C is improved by changing the side-chain conformations of active-site residues
Biochem. Biophys. Res. Commun.
310
19-27
2003
Brevundimonas diminuta
brenda
Kim, Y.; Hol, W.G.
Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity
Chem. Biol.
8
1253-1264
2001
Brevundimonas diminuta (Q9L5D6), Brevundimonas diminuta KAC-1 (Q9L5D6)
brenda
Kim, S.; Kim, Y.
Active site residues of cephalosporin acylase are critical not only for enzymatic catalysis but also for post-translational modification
J. Biol. Chem.
276
48376-48381
2001
Brevundimonas diminuta (Q9L5D6)
brenda
Aramori, I.; Fukagawa, M.; Tsumura, M.; Iwami, M.; Ono, H.; Ishitani, Y.; Koji, H.; Kohsaka, M.; Ueda, Y.; Imanaka, H.
Comparative characterization of new glutaryl 7-ACA and cephalosporin C acylases
J. Ferment. Bioeng.
73
185-192
1992
Brevibacillus laterosporus, Brevundimonas diminuta, Pseudomonas sp., Brevundimonas diminuta N176, Brevibacillus laterosporus J1
-
brenda
Kim, Y.; Yoon, K.; Khang, Y.; Turley, S.; Hol, W.G.
The 2.0 A crystal structure of cephalosporin acylase
Structure Fold. Des.
8
1059-1068
2000
Brevundimonas diminuta (Q9L5D6), Brevundimonas diminuta
brenda
Khatuntseva, S.A.; Eldarov, M.A.; Lopatin, S.A.; Zeinalov, O.A.; Skryabin, K.G.
Cloning and expression of variants of the glutaryl-7-aminocephalosporic acid acylase of the bacterium Brevundimonas diminuta in Escherichia coli cells
Appl. Biochem. Microbiol.
43
414-421
2007
Brevundimonas diminuta, Brevundimonas diminuta VKM B-1297
-
brenda
Sonawane, V.C.
Enzymatic modifications of cephalosporins by cephalosporin acylase and other enzymes
Crit. Rev. Biotechnol.
26
95-120
2006
Aeromonas sp., Achromobacter xylosoxidans, Arthrobacter sp., Rhizobium viscosum, Brevibacillus laterosporus, Bacillus sp. (in: Bacteria), Brevundimonas diminuta, Burkholderia cepacia, Sphingomonas paucimobilis, Flavobacterium sp., Paecilomyces sp. (in: Eurotiomycetes), Pseudomonas sp., Pseudomonas putida, Pseudomonas nitroreducens, Aeromonas sp. ACY 95, Burkholderia cepacia BY21, Arthrobacter sp. 5-8A, Flavobacterium sp. 650, Pseudomonas sp. A14, Brevibacillus laterosporus J1, Pseudomonas nitroreducens CCRC 11041, Bacillus sp. (in: Bacteria) SV12, Pseudomonas sp. BLO 72, Brevundimonas diminuta N 176
brenda
Khatuntseva, S.A.; Eldarov, M.A.; Redo, V.A.; Skryabin, K.G.
Purification and immobilization of recombinant variants of Brevundimonas diminuta glutaryl-7-aminocephalosporanic acid acylase expressed in Escherichia coli cells
J. Biotechnol.
133
123-126
2008
Brevundimonas diminuta
brenda
Ma, X.; Su, E.; Zhu, Y.; Deng, S.; Wei, D.
High-level expression of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas diminuta NK703 in Escherichia coli by combined optimization strategies
J. Biotechnol.
168
607-615
2013
Brevundimonas diminuta (G3MDB2), Brevundimonas diminuta, Brevundimonas diminuta NK703 (G3MDB2)
brenda
Ma, X.; Su, E.; Deng, S.; Wei, D.
An effective method for extraction of glutaryl-7-aminocephalosporanic acid acylase from recombinant E. coli cells
Biotechnol. Bioprocess Eng.
20
718-724
2015
Brevundimonas diminuta (G3MDB2), Brevundimonas diminuta NK703 (G3MDB2)
-
brenda