Information on EC 3.5.1.92 - pantetheine hydrolase

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The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY
3.5.1.92
-
RECOMMENDED NAME
GeneOntology No.
pantetheine hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(R)-pantetheine + H2O = (R)-pantothenate + 2-aminoethanethiol
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of amide bond
-
-
PATHWAY
KEGG Link
MetaCyc Link
Pantothenate and CoA biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
(R)-pantetheine amidohydrolase
The enzyme hydrolyses only one of the amide bonds of pantetheine. The substrate analogues phosphopantetheine and CoA are not substrates. The enzyme recycles pantothenate (vitamin B5) and produces 2-aminoethanethiol (cysteamine), a potent anti-oxidant [5].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
GPI-80
O95498
-
PAGEL
Q9NY84
-
pantetheinase
-
-
pantetheinase
-
-
pantetheinase
O95497, O95498, Q9NY84
-
pantetheinase
-
-
pantetheinase
-
-
pantetheinase-associated gene expressed in leukocytes
Q9NY84
-
pantetheinases
O95497, O95498, Q9N484
-
pantetheine hydrolase
-
-
vanin
-
-
vanin gene family
O95497, O95498, Q9N484
-
vanin-1
-
belongs to the nitrilase superfamily, which contains the invariant catalytic triad residues: glutamate, lysine, and cysteine
vanin-1
O95497
-
vanin-1
-
-
vanin-2
O95498
-
vanin-3
Q9N484
-
vanin-3
Q9NY84
-
vanin-3
-
-
VNN1
-
gene name
VNN1
O95497
-
VNN2
O95498
-
VNN3
Q9N484
-
VNN3
Q9NY84
-
CAS REGISTRY NUMBER
COMMENTARY
56093-18-6
-
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
vanin 1 knockout mice display an enhanced resistance to oxidative stress and show down-regulated tissue inflammation in response to oxidative stress. Vanin knockout mice exhibit a loss of cysteamine-mediated inhibition of peroxisome proliferator-activated receptor gamma. Vanin 1 deficient islets are twice as susceptible to cell death as wild type cells
physiological function
-
vanin plays a role in inflammation, oxidative stress, cell migration and numerous diseases including cardiovascular disease (increased vanin 1 activity has a protective effect against cardiovascular disease). Vanin 1 has a cytoprotective effect against type 1 diabetes in islet cells. Vanin 1 is a central regulator of not only stress responses via production of cysteamine but also lipid biosynthesis by controlling the flux through the fatty acid and/or cholesterol biosynthetic pathways
physiological function
-
overexpression of vanin-1 is most strongly associated with progression to chronic pediatric immune thrombocytopenia. Vanin-1 is a peripheral blood oxidative stress sensor
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-pantetheine + H2O
(R)-pantothenate + cysteamine
show the reaction diagram
-
-
-
-
?
(R)-pantetheine + H2O
(R)-pantothenate + cysteamine
show the reaction diagram
-
-
-
-
?
(R)-pantetheine 4-phosphate + H2O
?
show the reaction diagram
-
10% of the activity with (R)-pantetheine
-
-
?
(S)-ethyl pantetheine + H2O
?
show the reaction diagram
-
80% of the activity with (R)-pantetheine
-
-
?
6-O-[N-acetyl-alpha-D-glucosaminyl]-1-octadecylphospho-1D-myos-inositol + H2O
6-O-alpha-D-glucosaminyl-1-octadecylphospho-1D-myo-inositol + acetate
show the reaction diagram
-
-
-
-
?
N,N'-bis(pantothenyl)-1,6-diaminohexane + H2O
?
show the reaction diagram
-
96% of the activity with (R)-pantetheine
-
-
?
pantetheine + H2O
pantothenic acid + cysteamine
show the reaction diagram
-, O95497, O95498, Q9NY84
-
-
-
?
pantetheine + H2O
pantothenic acid + cysteamine
show the reaction diagram
Q9N484
-
-
-
?
pantetheine + H2O
pantothenic acid + cysteamine
show the reaction diagram
-
development of a highly sensitive fluorescent assay using a novel fluorescently labeled pantotheine derivative for high throughput screening for drug development and for quantification in various cell types and tissues
-
-
?
pantetheine + H2O
pantothenate + cysteamine
show the reaction diagram
-
-
-
-
?
pantetheine + H2O
pantothenate + cysteamine
show the reaction diagram
-
-
-
-
?
pantothenate-7-amido-4-methylcoumarin + H2O
pantothenic acid + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
based on LC-MS analysis
-
?
pantothenate-7-amido-4-methylcoumarin + H2O
pantothenic acid + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
based on LC-MS analysis, demonstrating that pantothenate-7-amido-4-methylcoumarin is hydrolyzed by vanin-1 at the amide-bond
-
?
pantothenyl-beta-aminoethanol + H2O
?
show the reaction diagram
-
-
-
-
?
pantothenyl-beta-aminoethanol + H2O
?
show the reaction diagram
-
75% of the activity with (R)-pantetheine
-
-
?
S-ethylpantetheine + H2O
?
show the reaction diagram
-
-
-
-
?
S-pantetheine 3-pyruvate + H2O
S-cysteamine 3-pyruvate + pantothenate
show the reaction diagram
-
-
S-cysteamine 3-pyruvate spontaneously forms a cyclic product
-
?
S-pantetheine-3-pyruvate + H2O
S-cysteamine 3-pyruvate + pantothenate
show the reaction diagram
-
-
-
-
?
S-pantetheine-3-pyruvate + H2O
S-cysteamine-3-pyruvate + pantothenate
show the reaction diagram
-
-
S-cysteamine-3-pyruvate cyclizes in a non-rate-limiting step to give 2H-1,4-thiazin-5,6-dihydro-3-carboxylic acid, a compound exhibiting a strong absorption at 296 nm
-
?
6-O-[N-acetyl-alpha-D-glucosaminyl]-1-[1,2-bis(hexadecanoyl)-sn-glycero]phosphor-1D-myo-inositol + H2O
6-O-alpha-D-glucosaminyl-1-[1,2-bis(hexadecanoyl)-sn-glycero]phospho-1D-myo-inositol + acetate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
membrane-bound pantetheinase is the main source of cysteamine in tissues under physiological conditions. The enzyme might by involved in the regulation of some immune functions maybe in the context of the response to oxidative stress
-
-
-
additional information
?
-
-
the transcription factors steroidogenic factor-1 and SOX9 regulate expression of Vanin-1 during mouse testis development. The sex- and cell-type-specific expression of vanin-1 in the developing mpouse gonad in vivo is required to provide an appropriate environment for male germ cell development
-
-
-
additional information
?
-
-
the vanin-1 gene is involved in testis development in mouse
-
-
-
additional information
?
-
-
vanin-1 is a key molecule to regulate the GSH-dependent response to oxidative injury in tissue at the epithelial level
-
-
-
additional information
?
-
-
vanin-1 plays a role in thymic reconstitution following damage by irradiation
-
-
-
additional information
?
-
-
vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions
-
-
-
additional information
?
-
-
vanin-1-/- mice are less susceptible to intestinal inflammation, either acute or chronic. Pantetheinase activity of vanin-1 is a major regulator of intestinal inflammation, acting through cysteamine release
-
-
-
additional information
?
-
-
vanin-1 controls granuloma formation and macrophage polarization in Coxiella burnetii infection
-
-
-
additional information
?
-
-
vanin-1 is cytoprotective for islet beta cells and regulates the development of type 1 diabetes
-
-
-
additional information
?
-
-
vanin-1 regulates chondrogenesis via glutathione metabolism and is critical for accelerated chondrogenesis of ank/anf mesenchymal precursors and phosphate donor-driven chondrogenic transdifferentiation and calcification of aortic smooth muscle cells
-
-
-
additional information
?
-
-
vanin-1, by antagonizing PPARgamma, licenses the production of inflammatory mediators by intestinal epithelial cells
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-O-[N-acetyl-alpha-D-glucosaminyl]-1-octadecylphospho-1D-myos-inositol + H2O
6-O-alpha-D-glucosaminyl-1-octadecylphospho-1D-myo-inositol + acetate
show the reaction diagram
-
-
-
-
?
pantetheine + H2O
pantothenic acid + cysteamine
show the reaction diagram
-, O95497, O95498, Q9NY84
-
-
-
?
pantetheine + H2O
pantothenic acid + cysteamine
show the reaction diagram
Q9N484
-
-
-
?
pantetheine + H2O
pantothenate + cysteamine
show the reaction diagram
-
-
-
-
?
pantetheine + H2O
pantothenate + cysteamine
show the reaction diagram
-
-
-
-
?
6-O-[N-acetyl-alpha-D-glucosaminyl]-1-[1,2-bis(hexadecanoyl)-sn-glycero]phosphor-1D-myo-inositol + H2O
6-O-alpha-D-glucosaminyl-1-[1,2-bis(hexadecanoyl)-sn-glycero]phospho-1D-myo-inositol + acetate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
-
-
-
-
additional information
?
-
-
membrane-bound pantetheinase is the main source of cysteamine in tissues under physiological conditions. The enzyme might by involved in the regulation of some immune functions maybe in the context of the response to oxidative stress
-
-
-
additional information
?
-
-
the transcription factors steroidogenic factor-1 and SOX9 regulate expression of Vanin-1 during mouse testis development. The sex- and cell-type-specific expression of vanin-1 in the developing mpouse gonad in vivo is required to provide an appropriate environment for male germ cell development
-
-
-
additional information
?
-
-
the vanin-1 gene is involved in testis development in mouse
-
-
-
additional information
?
-
-
vanin-1 is a key molecule to regulate the GSH-dependent response to oxidative injury in tissue at the epithelial level
-
-
-
additional information
?
-
-
vanin-1 plays a role in thymic reconstitution following damage by irradiation
-
-
-
additional information
?
-
-
vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions
-
-
-
additional information
?
-
-
vanin-1-/- mice are less susceptible to intestinal inflammation, either acute or chronic. Pantetheinase activity of vanin-1 is a major regulator of intestinal inflammation, acting through cysteamine release
-
-
-
additional information
?
-
-
vanin-1 controls granuloma formation and macrophage polarization in Coxiella burnetii infection
-
-
-
additional information
?
-
-
vanin-1 is cytoprotective for islet beta cells and regulates the development of type 1 diabetes
-
-
-
additional information
?
-
-
vanin-1 regulates chondrogenesis via glutathione metabolism and is critical for accelerated chondrogenesis of ank/anf mesenchymal precursors and phosphate donor-driven chondrogenic transdifferentiation and calcification of aortic smooth muscle cells
-
-
-
additional information
?
-
-
vanin-1, by antagonizing PPARgamma, licenses the production of inflammatory mediators by intestinal epithelial cells
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dithiothreitol
-
important for vanin-1 activity, vanin-1 activity is reduced in the absence of dithiothreitol and in the presence of a high level of 50 mM dithiothreitol. Optimal concentration: 0.4 mM
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,3-bis[(2-hydroxyethyl)thio]-1,4-naphthoquinone
-
i.e. NSC 95397, from library of pharmacologically active compounds, LOPAC
-
2-mercaptoethanol
-
reduced vanin-1 activity
2-Nitro-5-thiocyanobenzoate
-
0.1 mM, 15 min, 10% residual activity in absence of substrate S-pantetheine-3-pyruvate, 51% residual activity in presence of the substrate S-pantetheine-3-pyruvate
4,4'-dithiodipyridine
-
0.01 mM, 15 min, no residual activity in absence of substrate S-pantetheine-3-pyruvate, 13% residual activity in presence of the substrate S-pantetheine-3-pyruvate
4,4'-dithiodipyridine
-
competitive
5,5'-dithiobis(2-nitrobenzoate)
-
0.1 mM, 15 min, 78% residual activity in absence of substrate S-pantetheine-3-pyruvate, 33% residual activity in presence of the substrate S-pantetheine-3-pyruvate
5-(N,N-Hexamethylene)amiloride
-
from library of pharmacologically active compounds, LOPAC
8-cyclopentyl-1,3-didropylxanthine
-
from library of pharmacologically active compounds, LOPAC
-
beta-lapachone
-
from library of pharmacologically active compounds, LOPAC
Bromopyruvate
-
0.1 mM, 15 min, 2% residual activity in absence of substrate S-pantetheine-3-pyruvate, 76% residual activity in presence of the substrate S-pantetheine-3-pyruvate
cystamine
-
0.1 mM, 15 min, 41% residual activity in absence of substrate S-pantetheine-3-pyruvate, 60% residual activity in presence of the substrate S-pantetheine-3-pyruvate
cysteamine
-
5 mM, 55% inhibition
cystine
-
5 mM, 15 min, 100% residual activity in absence of substrate S-pantetheine-3-pyruvate, 32% residual activity in presence of the substrate S-pantetheine-3-pyruvate
dithiothreitol
-
vanin-1 activity is reduced in the absence of dithiothreitol and in the presence of a high level of 50 mM dithiothreitol
H2O2
-
0.1 mM, 15 min, 17% residual activity in absence of substrate S-pantetheine-3-pyruvate, 47% residual activity in presence of the substrate S-pantetheine-3-pyruvate
iodoacetamide
-
0.1 mM, 15 min, 0.5% residual activity in absence of substrate S-pantetheine-3-pyruvate, 72% residual activity in presence of the substrate S-pantetheine-3-pyruvate
iodoacetamide
-
at high concentrations
iodoacetate
-
1 mM, 15 min, 16% residual activity in absence of substrate S-pantetheine-3-pyruvate, 90% residual activity in presence of the substrate S-pantetheine-3-pyruvate
iodoacetate
-
at high concentrations
N2-(cis-2-aminocyclohexyl)-N6-(3-chlorophenyl)-9-ethyl-9H-purine-2,6-diamine hydrochloride
-
i.e. CGP-74514A hydrochloride, from library of pharmacologically active compounds, LOPAC
-
NaAsO2
-
1 mM, 15 min, 90% residual activity in absence or presence of substrate S-pantetheine-3-pyruvate
NaN3
-
15 mM, 90% inhibition
NEM
-
1 mM, 15 min, no residual activity in absence of substrate S-pantetheine-3-pyruvate, 54% residual activity in presence of the substrate S-pantetheine-3-pyruvate
o-Iodosobenzoate
-
0.05 mM, 15 min, 15% residual activity in absence of substrate S-pantetheine-3-pyruvate, 53% residual activity in presence of the substrate S-pantetheine-3-pyruvate
p-aminophenylarsine oxide
-
1 mM, 15 min, 45% residual activity in absence of substrate S-pantetheine-3-pyruvate, 55% residual activity in presence of the substrate S-pantetheine-3-pyruvate
pantethine
-
0.01 mM, 15 min, no residual activity in absence of substrate S-pantetheine-3-pyruvate, 52% residual activity in presence of the substrate S-pantetheine-3-pyruvate
pantethine
-
fully competitive at pH 5.0, uncompetitive at pH 8.0
pantethine
-
-
pantothenate
-
50 mM, 60% inhibition
PCMB
-
0.0001 mM, 15 min, 1% residual activity in absence of substrate S-pantetheine-3-pyruvate, 68% residual activity in presence of the substrate S-pantetheine-3-pyruvate
Phenylarsine oxide
-
1 mM, 15 min, 48% residual activity in absence of substrate S-pantetheine-3-pyruvate, 52% residual activity in presence of the substrate S-pantetheine-3-pyruvate
roscovitine
-
from library of pharmacologically active compounds, LOPAC
mercaptoethanol
-
-
additional information
-
no inhibition by 5 mM GSSG in absence or presence of substrate S-pantetheine-3-pyruvate
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
activates
cytokine IL-17
Q9N484
increased expression of vanin-1; increased expression vanin-3
-
cytokine Th1
Q9N484
increased expression of vanin-1; increased expression vanin-3
-
dithioerythritol
-
1-13 mM, activates
dithiothreitol
-
activates
additional information
Q9N484
19fold upregultion of gene expression of vanin-1 psoriasis, vanin-1 expression in atopic dermatits little increased; 2.3fold overexpression of vanin-3 in psioriais; induced expression of vanin-2 in psoriasis and atopic dermatitis; mixture of TNF-alpha, IL-1alpha and IL-6 results in increased expression of vanin-1 in reconstructed human epidermis; mixture of TNF-alpha, IL-1alpha and IL-6 results in increased expression of vanin-3 in reconstructed human epidermis
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.02
-
(R)-pantetheine
-
-
5
-
pantetheine
-
-
13
-
pantothenate-7-amido-4-methylcoumarin
-
apparent Km value, soluble recombinant vanin-1, pH 7.7, 25C
-
28
-
pantothenate-7-amido-4-methylcoumarin
-
apparent Km value, HPLC assay, pH 7.7, 25C
-
2.8e-05
-
S-cysteamine-3-pyruvate
-
pH 7.6, 37C
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.004
-
mercaptoethanol
-
-
0.0024
-
pantethine
-
pH 5.5
0.0035
-
pantethine
-
pH 8.0
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0057
-
2,3-bis[(2-hydroxyethyl)thio]-1,4-naphthoquinone
-
pH 7.5, 25C
-
0.00404
-
5-(N,N-Hexamethylene)amiloride
-
pH 7.5, 25C
0.0135
-
8-cyclopentyl-1,3-didropylxanthine
-
pH 7.5, 25C
-
0.0095
-
beta-lapachone
-
pH 7.5, 25C
0.0079
-
N2-(cis-2-aminocyclohexyl)-N6-(3-chlorophenyl)-9-ethyl-9H-purine-2,6-diamine hydrochloride
-
pH 7.5, 25C
-
0.0176
-
roscovitine
-
pH 7.5, 25C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
a simple, rapid, very sensitive, and specific continous spectrophotometric assay of pantetheinase activity
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
8
-
-
7.5
8.5
-
-
7.5
-
-
assay at
9
9.5
-
-
9
-
-
-
additional information
-
-
greatly reduced activity observed at both acidic (pH 3.0) and basic (pH 10.0) conditions
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
9.7
-
pH 5.5: about 50% of maximal activity, pH 9.7: about 70% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
epithelium. Vanin-1 controls the inflammatory response in injured colon. Vanin-1 regulates the expression of PPARgamma in epithelial cells
Manually annotated by BRENDA team
-
higher level in kidney than in liver, determined by RT-PCR
Manually annotated by BRENDA team
-
peripheral blood leukocyte
Manually annotated by BRENDA team
-
higher level in kidney than in liver, similar expression in lung, determined by RT-PCR
Manually annotated by BRENDA team
-
similar expression like in liver, determined by RT-PCR
Manually annotated by BRENDA team
-
lung epithelial cells, transfected and selected for stable expression of human vanin-1
Manually annotated by BRENDA team
Q9N484
normal skin, psoriatic lesions, atopic dermatitis lesions; normal skin, psoriatic lesions, atopic dermatitis lesions; normal skin, psoriatic lesions, atopic dermatitis lesions
Manually annotated by BRENDA team
-
the transcription factors steroidogenic factor-1 and SOX9 regulate expression of vanin-1 during mouse testis development
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
vanin-3 is secreted
-
Manually annotated by BRENDA team
-
the purified recombinant vanin-1 consists of a extracellular portion without a glycosylphosphatidylinositol linker
-
Manually annotated by BRENDA team
-
ectoenzyme, GPI-anchored pantetheinase
Manually annotated by BRENDA team
Q9N484
membrane-bound; membrane-bound; membrane-bound
Manually annotated by BRENDA team
-
an highly sensitive fluorescent assay using a novel fluorescent pantotheine derivative is used for characterization of a soluble version of human vanin-1 recombinant protein, identification of hits from high-throughput screening, and quantification of vanin pantotheinase acitivity in cell lines and tissues
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
54000
-
-
gel filtration
54000
-
-
size exclusion chromatography-multiangular light scattering (SEC-MALS)
55000
-
-
ultracentrifugation
72000
-
-
-
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 58000, SDS-PAGE
?
-
x * 60000, SDS-PAGE
monomer
-
1 * 60000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
-
glycoprotein
-
vanin-3
glycoprotein
-
-
glycoprotein
-
presence of galactose, mannose, fucose, glucose, galactosamine and sialic acid, 11.8% total carbohydrate content
glycoprotein
-
11.8% carbohydrate by weight
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
7.6
-
good stability, rapid decrease outside this range
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
after solubilization, the enzsme can be stored indefinitely in the frozen state without significant loss of activity
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, pH 7, several months without significant loss of activity
-
0C, dilute solutions lose 5% activity per week
-
-20C, 12 months without loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant enzyme,His- and Flag-tagged vanin-1 is purified over a 10 ml HisTrap Fast Flow column (a nickel affinity chromatography), 95% purity
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in CHO cells
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EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
in lipopolysaccharide-treated cells, vanin-1 expression increases 5.1 to 40.2fold. In sodium arsenite-treated cells, vanin-1 expression increases 1.9 to 39.8fold
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the mRNA expression of vanin-1 increases about 2.4fold after the exposure of renal tubular cells to organic solvents (allyl alcohol, ethylene glycol, formaldehyde, chloroform, and phenol) for 24 h
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the mRNA level of vanin-1 significantly elevates in the renal cortices of ethylene glycol-exposed rats
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APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
drug development
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the highly sensitive fluorescent assay for the hydrolysis of pantothenate-7-amido-4-methylcoumarin as substrate to pantothenic acid and 7-amino-4-methylcoumarin could be a powerful tool for target validation and drug lead identification and characterization
medicine
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vanin-1 is a potential biomarker for nephrotoxicant-induced renal injury
medicine
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the pantetheinase activity of vanin-1 molecule could be a target for a new anti-inflammatory strategy
medicine
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manipulation the Vanin-1/pantetheinase activity represents an attractive therapeutic strategy for the treatment of inflammatory bowel disease
medicine
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vanin-1 is a potential biomarker for nephrotoxicant-induced renal injury