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EC Tree
The taxonomic range for the selected organisms is: Mus musculus The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
kynurenine formamidase, afmid, formylase, kfase, formamidase i, formamidase ii, arylformamidase, formylkynurenine formamidase,
more
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formylkynureninase
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formylkynurenine formamidase
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kynurenine formamidase
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kynurine formamidase
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N-formyl-L-kynurenine + H2O = formate + L-kynurenine
the catalytic triad is formed by residues Ser162, Asp247, and His279
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carboxylic acid amide hydrolysis
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aryl-formylamine amidohydrolase
Also acts on other aromatic formylamines.
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N-formyl-L-kynurenine + H2O
formate + L-kynurenine
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N-formyl-L-kynurenine + H2O
formate + L-kynurenine
additional information
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an enzyme other than the aryl formamidase additionally catalyzes the formation of L-kynurenine in kidney
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N-formyl-L-kynurenine + H2O
formate + L-kynurenine
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N-formyl-L-kynurenine + H2O
formate + L-kynurenine
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step in tryptophan metabolism and biosynthesis of kynurenine-derived derivatives, e.g. kynurenic acid, quinolinic acid, nicotinamide, NAD+, and NADP+, pathway disruption causes neurotoxicity and immunotoxicity, overview
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N-formyl-L-kynurenine + H2O
formate + L-kynurenine
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?
N-formyl-L-kynurenine + H2O
formate + L-kynurenine
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step in tryptophan metabolism and biosynthesis of kynurenine-derived derivatives, e.g. kynurenic acid, quinolinic acid, nicotinamide, NAD+, and NADP+, pathway disruption causes neurotoxicity and immunotoxicity, overview
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additional information
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an enzyme other than the aryl formamidase additionally catalyzes the formation of L-kynurenine in kidney
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0.18 - 0.19
N-formyl-L-kynurenine
0.18
N-formyl-L-kynurenine
pH 7.4, native wild-type enzyme
0.19
N-formyl-L-kynurenine
pH 7.4, recombinant wild-type enzyme
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42
purified recombinant wild-type enzyme
0.00002
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liver knockout mutant enzyme
0.00087
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kidney knockout mutant enzyme
0.0038
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kidney heterozygous mutant enzyme
0.0067
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kidney wild-type enzyme
0.0083
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liver heterozygous mutant enzyme
0.0156
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liver wild-type enzyme
additional information
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determination of kynurenine pathway metabolites concentrations in plasma of wild-type and mutant mice
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SwissProt
brenda
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brenda
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brenda
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brenda
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high activity
brenda
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brenda
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KFA_MOUSE
305
0
34229
Swiss-Prot
other Location (Reliability: 4 )
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34229
x * 34229, sequence calculation
36000
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formamidase II, gel filtration
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?
x * 34229, sequence calculation
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D247A
site-directed mutagenesis, inactive mutant
H279A
site-directed mutagenesis, inactive mutant
S162A
site-directed mutagenesis, inactive mutant
additional information
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construction of heterozygous and knockout enzyme-deficient mutants, inactivation through the removal of a shared bidirectional promoter region regulating expression of the Afmid gene and the thymidine kinase gene, phenotype overview, Afmid knockout mice show 13% residual enzyme activity in kidney due to a distinct enzyme activity
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native enzyme from liver cytosol, recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography, gel filtration, and nickel affinity chromatography, to high purity, recombinant wild-type enzyme 90fold
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DNA and amino acid sequence determination and anlysis, expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain DH10B
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Moore, G.P.; Sullivan, D.T.
The characterization of multiple forms of kynurenine formidase in Drosophila melanogaster
Biochim. Biophys. Acta
397
468-477
1975
Bos taurus, Saccharomyces cerevisiae, Gallus gallus, Drosophila melanogaster, Drosophila virilis, Mus musculus, Musca domestica, Lithobates pipiens
brenda
Dobrovolsky, V.N.; Bowyer, J.F.; Pabarcus, M.K.; Heflich, R.H.; Williams, L.D.; Doerge, D.R.; Arvidsson, B.; Bergquist, J.; Casida, J.E.
Effect of arylformamidase (kynurenine formamidase) gene inactivation in mice on enzymatic activity, kynurenine pathway metabolites and phenotype
Biochim. Biophys. Acta
1724
163-172
2005
Mus musculus
brenda
Pabarcus, M.K.; Casida, J.E.
Cloning, expression, and catalytic triad of recombinant arylformamidase
Protein Expr. Purif.
44
39-44
2005
Mus musculus (Q8K4H1), Mus musculus
brenda