Information on EC 3.5.1.9 - arylformamidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.5.1.9
-
RECOMMENDED NAME
GeneOntology No.
arylformamidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
N-formyl-L-kynurenine + H2O = formate + L-kynurenine
show the reaction diagram
also acts on other aromatic formylamines
-
-
-
N-formyl-L-kynurenine + H2O = formate + L-kynurenine
show the reaction diagram
the catalytic triad is formed by residues Ser162, Asp247, and His279
Q8K4H1
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
carboxylic acid amide hydrolysis
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Glyoxylate and dicarboxylate metabolism
-
Metabolic pathways
-
tryptophan degradation I (via anthranilate)
-
tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
-
tryptophan degradation XI (mammalian, via kynurenine)
-
Tryptophan metabolism
-
SYSTEMATIC NAME
IUBMB Comments
aryl-formylamine amidohydrolase
Also acts on other aromatic formylamines.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
AFMID
-
-
AFMID
Q8K4H1
-
formamidase I
-
-
-
-
formamidase II
-
-
-
-
formyl kynurenine formamidase
-
-
formylase
-
-
-
-
formylkynureninase
-
-
-
-
formylkynurenine formamidase
-
-
-
-
KFase
-
-
kynurenine formamidase
-
-
kynurenine formamidase
-
-
kynurine formamidase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
156229-75-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain ATCC 14579
-
-
Manually annotated by BRENDA team
formamidase I
-
-
Manually annotated by BRENDA team
guinea pig, formamidase II
-
-
Manually annotated by BRENDA team
1form, formamidase I
-
-
Manually annotated by BRENDA team
1 form, formamidase I
-
-
Manually annotated by BRENDA team
2 forms, formamidase I and II
-
-
Manually annotated by BRENDA team
2 forms, formamidase I and II
-
-
Manually annotated by BRENDA team
chicken, formamidase I
-
-
Manually annotated by BRENDA team
Hansenula henricii
2 forms, formamidase I and II
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
formamidase II
-
-
Manually annotated by BRENDA team
gene Afmid
-
-
Manually annotated by BRENDA team
housefly, formamidase I
-
-
Manually annotated by BRENDA team
no activity in Mus musculus
not present in liver
-
-
Manually annotated by BRENDA team
no activity in Rattus sp.
not present in liver
-
-
Manually annotated by BRENDA team
rainbow trout
-
-
Manually annotated by BRENDA team
rabbit, formamidase II
-
-
Manually annotated by BRENDA team
strain PAO1
-
-
Manually annotated by BRENDA team
frog, formamidase II
-
-
Manually annotated by BRENDA team
1 form, formamidase I
-
-
Manually annotated by BRENDA team
formamidase II
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2-nitrophenyl)acetate + H2O
2-nitrophenol + acetic acid
show the reaction diagram
-
-
-
?
2-chloro-N-(1-naphthyl)acetamide + H2O
chloroacetate + 1-naphthylamine
show the reaction diagram
-
low activity
-
?
2-methylformanilide + H2O
2-methylaniline + formate
show the reaction diagram
-
-
-
-
?
5-hydroxyformyl-DL-kynurenine + H2O
5-hydroxy-DL-kynurenine + formate
show the reaction diagram
-
-
-
?
acetanilide + H2O
acetate + aniline
show the reaction diagram
-
formation of alcohol esters in the presence of alcohols
-
?
N-acetylanthranilic acid + H2O
acetate + anthranilic acid
show the reaction diagram
-
formation of alcohol esters in the presence of alcohols
-
?
N-formyl-2aminobenzenesulfonic acid + H2O
formate + 2-aminobenzenesulfonic acid
show the reaction diagram
-
low activity
-
?
N-formyl-4-(methylamino)-benzoic acid + H2O
4-methylaminobenzoic acid + formate
show the reaction diagram
-
-
-
-
?
N-formyl-DL-kynurenine + H2O
L-kynurenine + formate + N-formyl-D-kynurenine
show the reaction diagram
-
mixture of equal amounts of D- and L-kynurenine
N-formyl-D-kynurenine might inhibit activity
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
Q04066
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
Hansenula henricii
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
preferred substrate, involved in formation of antibiotics and NAD
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
formation of alcohol esters in the presence of alcohols
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
formamidase II is an integral component of the actinomycin-synthesizing system
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
unable to transfer a formyl group directly to tetrahydrofolic acid
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
involved in the pathway of tryptophan degradation
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
-
?
N-formyl-L-kynurenine + H2O
formate + L-kynurenine
show the reaction diagram
-
-
-
-
?
N-formyl-L-kynurenine + H2O
formate + L-kynurenine
show the reaction diagram
Q8K4H1
-
-
-
?
N-formyl-L-kynurenine + H2O
formate + L-kynurenine
show the reaction diagram
-
step in tryptophan metabolism and biosynthesis of kynurenine-derived derivatives, e.g. kynurenic acid, quinolinic acid, nicotinamide, NAD+, and NADP+, pathway disruption causes neurotoxicity and immunotoxicity, overview
-
-
?
N-formyl-m-aminobenzoic acid + H2O
formate + m-aminobenzoic acid
show the reaction diagram
-
very low activity
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
-
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
low activity
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
the only substrate
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
60% of activity compared to N-formyl-L-kynurenine
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
22% of activity compared to N-formyl-L-kynurenine
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
low activity for both formamidases I and II
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
low activity for both formamidases I and II
-
?
N-formyl-o-aminonitrobenzene + H2O
formate + o-nitroaniline
show the reaction diagram
-
low activity
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
-
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
-
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
low activity
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
formation of alcohol esters in the presence of alcohols
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
24% of activity compared to N-formyl-L-kynurenine
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
involvement in anthranilate cycle
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
13.5% activity compared to N-formyl-L-kynurenine
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
Hansenula henricii
-
10times slower hydrolysis than for N-formyl-L-kynurenine
-
?
N-formylnitroaniline + H2O
formate + nitroaniline
show the reaction diagram
-
low activity
-
?
N1,Nalpha-diformyl-L-kynurenine + H2O
formate + N-alpha-L-kynurenine
show the reaction diagram
-
-
-
?
N1,Nalpha-diformyl-L-kynurenine + H2O
formate + N-alpha-L-kynurenine
show the reaction diagram
-
-
-
?
N1,Nalpha-diformyl-L-kynurenine + H2O
formate + N-alpha-L-kynurenine
show the reaction diagram
-
lower activity for formamidases I and II than for its natural substrate
-
?
N1,Nalpha-diformyl-L-kynurenine + H2O
formate + N-alpha-L-kynurenine
show the reaction diagram
-
lower activity for formamidases I and II than for its natural substrate
-
?
formanilide + H2O
formate + aniline
show the reaction diagram
-
poor activity
-
?
additional information
?
-
-
an enzyme other than the aryl formamidase additionally catalyzes the formation of L-kynurenine in kidney
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
Hansenula henricii
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
-
-
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
preferred substrate, involved in formation of antibiotics and NAD
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
formation of alcohol esters in the presence of alcohols
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
formamidase II is an integral component of the actinomycin-synthesizing system
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
unable to transfer a formyl group directly to tetrahydrofolic acid
-
?
N-formyl-L-kynurenine + H2O
L-kynurenine + formate
show the reaction diagram
-
involved in the pathway of tryptophan degradation
-
?
N-formyl-L-kynurenine + H2O
formate + L-kynurenine
show the reaction diagram
Q8K4H1
-
-
-
?
N-formyl-L-kynurenine + H2O
formate + L-kynurenine
show the reaction diagram
-
step in tryptophan metabolism and biosynthesis of kynurenine-derived derivatives, e.g. kynurenic acid, quinolinic acid, nicotinamide, NAD+, and NADP+, pathway disruption causes neurotoxicity and immunotoxicity, overview
-
-
?
N-formyl-m-aminobenzoic acid + H2O
formate + m-aminobenzoic acid
show the reaction diagram
-
very low activity
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
-
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
low activity
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
the only substrate
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
60% of activity compared to N-formyl-L-kynurenine
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
22% of activity compared to N-formyl-L-kynurenine
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
low activity for both formamidases I and II
-
?
N-formyl-o-aminoacetophenone + H2O
formate + o-aminoacetophenone
show the reaction diagram
-
low activity for both formamidases I and II
-
?
N-formyl-o-aminonitrobenzene + H2O
formate + o-nitroaniline
show the reaction diagram
-
low activity
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
-
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
-
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
low activity
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
formation of alcohol esters in the presence of alcohols
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
24% of activity compared to N-formyl-L-kynurenine
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
involvement in anthranilate cycle
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
-
13.5% activity compared to N-formyl-L-kynurenine
-
?
N-formylanthranilic acid + H2O
formate + anthranilic acid
show the reaction diagram
Hansenula henricii
-
10times slower hydrolysis than for N-formyl-L-kynurenine
-
?
5-hydroxyformyl-DL-kynurenine + H2O
5-hydroxy-DL-kynurenine + formate
show the reaction diagram
-
-
-
?
additional information
?
-
-
an enzyme other than the aryl formamidase additionally catalyzes the formation of L-kynurenine in kidney
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
Q04066
dependent
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co3+
-
15-20fold activation
Mn2+
-
15-20fold activation
additional information
-
no activation by Mg2+, Cu2+ and Co3+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1-pyridine-2-aldoxime 1'-(pyridine-4-aldoxime) dimethyl ether dichloride
-
HS-3, complete inhibition at 0.5 mM
2-pyridine aldoxime
-
31% inhibition at 1 mM
3-Hydroxy-DL-kynurenine
-
50% inhibition at 0.2 mM, Ki: 8 mM
3-pyridine aldoxime O-n-propyl ether
-
43% inhibition at 1 mM
3-pyridine aldoxime O-phenyl ether
-
61% inhibition at 1 mM
acetone
-
inhibition up to 10%, v/v
Ag+
-
complete inhibition at 0.01 mM
Ag+
-
76% inhibition at 0.1 mM
Ag+
-
complete inhibition at 0.1 mM
Ag+
-
complete inhibition at 0.05 mM
anthranilic acid
-
9% inhibition at 0.5-1 mM
anthranilic acid
-
29% inhibition at 1-2 mM
anthranilic acid
-
Ki: 1.6 mM
anthranilic acid
-
45% inhibition at 0.5 mM
arsenate
-
strong inhibition for formamidase I, slight inhibition for formamidase II at 1 mM
ascorbate
-
50% inhibition at 10 mM
Ba2+
-
strong inhibition at 1 mM
bis(4-nitrophenyl)phosphate
-
slight inhibition at 1 mM after 30 min preincubation
bromphenol blue
-
50% inhibition at 0.024 mM after 5 min preincubation
Carbaryl
-
moderately potent teratogen, Kd: 0.085 mM
Co2+
-
moderate inhibition at 1 mM
Cu2+
-
moderate inhibition at 1 mM
Cu2+
-
complete inhibition at 1 mM
Cu2+
Hansenula henricii
-
strong inhibition at 0.25 mM, more inhibition for formamidase I than formamidase II
Cu2+
-
21% inhibition at 0.1 mM
Cu2+
-
31% inhibition at 1 mM
diazoxon
-
highly potent teratogen, Kd: 0.15 mM
dicrotophos
-
potent teratogen, Kd: 3.2 mM
diethyl dicarbonate
-
90% inhibition at 0.5 mM
diethyl-(4-nitrophenyl)-phosphate
-
marked inhibition
diisopropylfluorophosphate
-
complete inhibition at 0.01 mM
dimethyl sulfoxide
-
inhibition up to 10%, v/v
Dioxane
-
inhibition up to 10%, v/v
EDTA
-
0.1 mM, complete inactivation
Fe2+
-
31% inhibition only for formamidase II at 1 mM
-
Hg2+
-
moderate inhibition at 1 mM
Hg2+
-
strong inhibition at 1 mM
Hg2+
-
complete inhibition at 1 mM
Hg2+
Hansenula henricii
-
strong inhibition for both, formamidases I and II
Hg2+
-
50% inhibition at 0.1 mM
Hg2+
-
20% inhibition at 0.05 mM
Hg2+
-
57% inhibition at 0.1 mM
Hg2+
-
complete inhibition at 1 mM
Hg2+
-
complete inhibition at 0.05 mM
hydrosulfite
-
strong inhibition at 1 mM
iodoacetamide
Hansenula henricii
-
slight inhibition at 1 mM for both formamidases I and II
Isoleucine
-
19% inhibition at 20 mM
L-kynurenine
-
marked inhibition above 0.1 mM
L-kynurenine
-
competitive inhibition, Ki: 0.26 mM
L-kynurenine
-
15% inhibition at 0.1 mM, Ki: 0.25 mM
L-kynurenine
-
strong non-competitive inhibition
L-tryptophan
-
non-competitive inhibition, Ki: 5.24 mM
methionine
-
20% inhibition at 20 mM
methyl-triphenyl phosphonium bromide
-
-
Mg2+
-
moderate inhibition at 1 mM
Mn2+
-
strong inhibition at 1 mM
N,N-Dimethylformamide
-
inhibition up to 10%, v/v
N-acetylanthranilic acid
-
15% inhibition at 6.7 mM
N-bromosuccinimide
-
marked inhibition
N-ethyloxycarbonyl-2-ethyloxy-1,2-dihydroquinoline
-
53% inhibition at 1 mM
N-formyl-o-aminotoluene
-
45% inhibition at 6.7 mM
Na-bisulfite
-
50% inhibition at 0.18 mM
Na-bisulfite
-
50% inhibition at 0.32 mM
Na-bisulfite
-
strong inhibition 10 mM
Na-bisulfite
-
complete inhibition at 4 mM
Na-Metaarsenite
-
marked inhibition
Na2S2O4
-
27% inhibition at 0.04 mM
NaAsO2
-
marked inhibition
NaAsO2
-
25% inhibition at 0.05 mM
NaCN
-
31% inhibition at 5 mM
NaF
-
at 10 mM: strong inhibition for formamidase I, slight inhibition for formamidase II
NaF
-
42% inhibition at 5 mM
NaF
Hansenula henricii
-
strong inhibition for both formamidases I and II at 1 mM
NaF
-
27% inhibition at 10 mM
NaHSO3
-
50% inhibition at 0.18 mM
NaHSO3
-
50% inhibition at 0.32 mM
NaHSO3
-
strong inhibition at at 10 mM
NaHSO3
Hansenula henricii
-
strong inhibition at 1 mM for both formamidases I and II
NaHSO3
-
24% inhibition at 0.04 mM
NaHSO3
-
complete inhibition at 4 mM
NH2OH*HCl
-
14% inhibition at 3 mM
O,O-Diethyl-O-(4-nitrophenyl)phosphate
-
E600, complete inhibition at 1 mM
o-aminobenzamidine
-
62% inhibition at 0.5 mM
p-chloromercuribenzoate
Hansenula henricii
-
strong inhibition at 0.1 mM for both formamidases I and II
p-hydroxymercuribenzoate
-
complete inhibition at 1 mM
p-nitrophenyldiethyl phosphoric acid
-
92% inhibition at 10 mM
-
phenyl saligenin cyclic phosphate
-
Kd: 0.00083 mM
phenylalanine
-
slight inhibition at 20 mM
phenylmethylsulfonyl fluoride
-
strong inhibition
phenylmethylsulfonyl fluoride
-
Kd: 3.3 mM
phenylmethylsulfonyl fluoride
-
complete inhibition at 1 mM
Salicyl aldoxime
-
71% inhibition at 1 mM
tetraethyl diphosphate
-
Kd: 67 mM
tetraheptylammonium bromide
-
94% inhibition at 2.5 mM
tetrahexylammonium bromide
-
56% inhibition at 5 mM
tetraoctylammoniumbromide
-
94% inhibition at 2.5 mM
tetraphenyl phosphonium bromide
-
22% inhibition at 5 mM
threonine
-
19% inhibition at 20 mM
trimethyl-n-decylammonium bromide
-
65% inhibition at 5 mM
trimethyl-n-decylammonium chloride
-
65% inhibition at 5 mM
trimethyl-n-hexadecylammonium bromide
-
100% inhibition at 5 mM
trimethylhexadecylammonium chloride
-
100% inhibition at 5 mM
trimethylphenylammonium bromide
-
20% inhibition at 5 mM
trimethylphenylammonium chloride
-
20% inhibition at 5 mM
Urea
-
75% inhibition at 1 M
Urea
-
25% inhibition at 2 M
valine
-
slight inhibition at 20 mM
Zn2+
-
moderate inhibition at 1 mM
Zn2+
-
strong inhibition at 1 mM
Zn2+
-
complete inhibition of formamidase I at 1 mM, 88% inhibition of formamidase II at 1 mM
Zn2+
Hansenula henricii
-
strong inhibition at 0.1 mM, more for formamidase II than formamidase I
Monocrotophos
-
potent teratogen, Kd: 5.4 mM
additional information
-
no inhibition by PMSF
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
arginine
-
30% activation at 20 mM
ascorbate
-
57% activation at 100 mM
butanol
-
activation e.g. between 0.75-2.5%, v/v, depending on substrate
ethanol
-
activation e.g. between 2.5-12%, v/v, depending on substrate
L-tryptophan
-
activation from 5-20 mM
lysine
-
30% activation at 20 mM
methanol
-
optimal activation e.g. between 5-15%, v/v, depending on substrate
methionine
-
20% activation at 20 mM
o-aminophenol
-
activation at 0.2 mM
Propanol
-
activation e.g. between 1.5-5%, v/v, depending on substrate
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1
-
2-Chloro-N-(1-naphthyl)acetamide
-
-
0.4
-
5-hydroxy-DL-kynurenine
-
-
0.45
-
N-formyl-DL-kynurenine
-
-
0.05
-
N-formyl-L-kynurenine
-
-
0.06
-
N-formyl-L-kynurenine
-
-
0.11
-
N-formyl-L-kynurenine
-
-
0.14
-
N-formyl-L-kynurenine
-
-
0.18
-
N-formyl-L-kynurenine
-
-
0.18
-
N-formyl-L-kynurenine
Q8K4H1
pH 7.4, native wild-type enzyme
0.18
-
N-formyl-L-kynurenine
Q04066
-
0.19
-
N-formyl-L-kynurenine
Q8K4H1
pH 7.4, recombinant wild-type enzyme
0.28
-
N-formyl-L-kynurenine
-
-
0.31
-
N-formyl-L-kynurenine
-
formamidase II
0.34
-
N-formyl-L-kynurenine
-
formamidase I
0.78
-
N-formyl-L-kynurenine
-
-
0.83
-
N-formyl-L-kynurenine
-
formamidase II
1.87
-
N-formyl-L-kynurenine
-
formamidase I
1.9
-
N-formyl-L-kynurenine
Hansenula henricii
-
formamidase I
2.2
-
N-formyl-L-kynurenine
Hansenula henricii
-
formamidase II
2.31
-
N-formyl-L-kynurenine
-
formamidase II
5
-
N-formyl-L-kynurenine
-
formamidase I
7.7
-
N-formyl-m-aminobenzoic acid
-
-
2.8
-
N-formyl-o-aminoacetophenone
-
-
8.1
-
N-formyl-o-aminoacetophenone
-
-
2.5
-
N-formyl-o-aminonitrobenzene
-
-
0.21
-
N-formylanthranilic acid
-
-
0.57
-
N-formylanthranilic acid
-
-
3.3
-
N-formylanthranilic acid
-
-
0.39
-
N1,Nalpha-diformyl-L-kynurenine
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
161
-
N-formyl-L-kynurenine
Q04066
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
8
-
3-Hydroxy-DL-kynurenine
-
-
10
-
ascorbate
-
-
0.024
-
bromphenol blue
-
after 5 min preincubation
0.1
-
Hg2+
-
-
0.25
-
L-kynurenine
-
-
5.24
-
L-tryptophan
-
non-competitive inhibition
0.18
-
Na-bisulfite
-
-
0.32
-
Na-bisulfite
-
-
0.18
-
NaHSO3
-
-
0.32
-
NaHSO3
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00002
-
-
liver knockout mutant enzyme
0.00087
-
-
kidney knockout mutant enzyme
0.0038
-
-
kidney heterozygous mutant enzyme
0.0067
-
-
kidney wild-type enzyme
0.0083
-
-
liver heterozygous mutant enzyme
0.0156
-
-
liver wild-type enzyme
0.26
-
-
formamidase I
0.33
-
-
-
0.48
-
-
formamidase II
0.59
-
Hansenula henricii
-
-
0.8
-
-
-
3.5
-
-
1 mM N-formyl-L-kynurenine, pH 7.4, 37C
4.7
-
-
1 mM N-formyl-L-kynurenine, pH 7.4, 37C
5.9
-
-
1 mM N-formyl-L-kynurenine, pH 7.4, 37C
8.33
-
-
formamidase I
22.8
-
-
-
26.8
-
-
formamidase II
42
-
Q8K4H1
purified recombinant wild-type enzyme
80.4
-
-
-
303
-
-
-
346
-
-
-
425
-
-
-
additional information
-
-
determination of kynurenine pathway metabolites concentrations in plasma of wild-type and mutant mice
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
7
Hansenula henricii
-
formamidase II
5.5
8.5
-
-
6
9
-
-
6.5
8
-
formamidase II
6.5
-
-
-
6.7
7.8
-
formamidase I
7
8
-
formamidase I
7
8
Hansenula henricii
-
formamidase I
7
-
-
formamidase II
7.4
-
-
assay at
7.4
-
Q8K4H1
assay at
8
-
-
-
8.5
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
9
-
optimum range
6
8.5
-
active within
6.2
9.3
-
high activity within
6.5
11
-
high activity within
6.5
7.5
-
high activity within
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
formamidase II
50
-
-
formamidase I
60
-
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35
60
-
activity constant within
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Hansenula henricii
-
decreasing formamidase synthesis in presence of anthranilic acid, p-aminobenzoic acid, 3-hydroxyanthranilic acid, kynurenine or 3-hydroxykynurenine
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
-
2 forms: formamidase I, formamidase II
Manually annotated by BRENDA team
-
temporal expression and extent of formamidase II due to synthesis of components of actinomycin-synthesizing enzymes
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25000
-
-
formamidase II, gel filtration
27000
-
-
formamidase II, gel filtration
28000
-
Hansenula henricii
-
formamidase II, gel filtration
30000
-
-
formamidase II, gel filtration
30500
-
-
formamidase II, gel filtration
31000
-
-
formamidase II, gel filtration
31700
-
-
gel filtration
34700
-
-
gel filtration
34900
-
-
SDS-PAGE
36000
-
-
formamidase II, gel filtration
36500
-
-
formamidase II, gel filtration
42600
-
-
gel filtration
43000
-
-
formamidase I, gel filtration
54000
-
-
formamidase I, gel filtration
56000
-
Hansenula henricii
-
formamidase I, gel filtration
56000
-
-
formamidase I, gel filtration
57000
-
-
formamidase I, gel filtration
58500
-
-
formamidase I, gel filtration
59000
-
-
formamidase I, gel filtration
59000
-
-
formamidase I, gel filtration
59000
-
-
gel filtration
60000
-
-
gel filtration
60000
-
-
formamidase I, gel filtration
60000
-
-
formamidase I, gel filtration
60000
-
-
-
60000
-
-
formamidase I, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Q8K4H1
x * 34229, sequence calculation
dimer
-
2 * 34000, formamidase I, SDS-PAGE
dimer
-
2 * 30000, SDS-PAGE
monomer
-
1 * 35500, SDS-PAGE
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3
4
-
remaining activity: 20-50%
3
4
-
no activity
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
activity of formamidase II declines sharp at higher temperatures
45
-
Hansenula henricii
-
50% activity after 40 min for formamidase I, complete inactivation for formamidase II
56
-
-
30% inactivation after 2 min
60
-
-
80% remaining activity after 30 min
60
-
-
16% remaining activity after 30 min
60
-
-
sharp decline of activity
65
-
-
inactivation in 35 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ionic strength affects enzyme activity
-
formamidase I more heat-stable than formamidase II
-
sensitive to repeated freezing and thawing
-
ionic strength affects enzyme activity
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
4C, 60% of activity recovered after seven months
-
5C, no inactivation during prolonged storage
-
4C, fully active within seven months
-
0-2C, 3 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
to homogeneity, chromatography techniques
-
to homogeneity, chromatography techniques, 2 forms
-
to homogeneity: formamidase I, partial: formamidase II, chromatography techniques
-
near homogeneity, chromatography techniques
-
to homogeneity, chromatography techniques, preparative disc gel electrophoresis
-
partial
Hansenula henricii
-
native enzyme from liver cytosol, recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography, gel filtration, and nickel affinity chromatography, to high purity, recombinant wild-type enzyme 90fold
Q8K4H1
to homogeneity, chromatography techniques
-
near homogeneity, chromatography techniques, isoelectric focusing
-
partial
-
to homogeneity, chromatography steps
-
to homogeneity, chromatography steps, isoelectric focusing
-
to homogeneity, chromatography techniques, 2 forms
-
to homogeneity, chromatography techniques
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
kynB gene, expression in Escherichia coli as N-terminal His6 tag fusions
-
expression of putative kynBAU in Escherichia coli
-
DNA and amino acid sequence determination and anlysis, expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain DH10B
Q8K4H1
kynB gene, expression in Escherichia coli as N-terminal His6 tag fusions
-
expressed in Escherichia coli
Q04066
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
kynurenine formamidase shows enhanced expression in the forewing band of Heliconius melpomene during forewing ommochrome pigmentation with highest levels of expression in LM wings, and specifically in the distal wing region. Kynurenine formamidase is also upregulated in the early melanin band region
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D247A
Q8K4H1
site-directed mutagenesis, inactive mutant
S162A
Q8K4H1
site-directed mutagenesis, inactive mutant
H279A
Q8K4H1
site-directed mutagenesis, inactive mutant
additional information
-
construction of heterozygous and knockout enzyme-deficient mutants, inactivation through the removal of a shared bidirectional promoter region regulating expression of the Afmid gene and the thymidine kinase gene, phenotype overview, Afmid knockout mice show 13% residual enzyme activity in kidney due to a distinct enzyme activity
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
degradation
-
aerobic degradation of L-tryptophan
degradation
-
aerobic degradation of L-tryptophan
-
degradation
-
aerobic degradation of L-tryptophan