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Information on EC 3.5.1.89 - N-acetylglucosaminylphosphatidylinositol deacetylase and Organism(s) Homo sapiens and UniProt Accession Q9Y2B2
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3.5.1.89 N-acetylglucosaminylphosphatidylinositol deacetylaseIUBMB Comments
Involved in the second step of glycosylphosphatidylinositol (GPI) anchor formation in all eukaryotes. The enzyme appears to be composed of a single subunit (PIG-L in mammalian cells and GPI12 in yeast). In some species, the long-chain sn-1-acyl group of the phosphatidyl group is replaced by a long-chain alkyl or alk-1-enyl group.
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Word Map
- 3.5.1.89
- glycosylphosphatidylinositol
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trypanosomal
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glcn-pi
- sickness
-
gpi-anchored
-
de-n-acetylation
- alpha-mannosyltransferase
- medicine
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mannosylated
- drug development
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
Synonyms
glcnac-pi de-n-acetylase, pig-l, gpi12, tbgpi12, cagpi12, pig-l protein, denac, n-acetylglucosaminylphosphatidylinositol deacetylase, glcnac-pi-de-n-acetylase, n-acetylglucosaminylphosphatidylinositol de-n-acetylase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Acetylglucosaminylphosphatidylinositol deacetylase
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-
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Deacetylase, acetylglucosaminylphosphatidylinositol
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-
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GlcNAc-PI deacetylase
N-Acetylglucosaminylphosphatidylinositol de-N-acetylase
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-
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GlcNAc-PI deacetylase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of carboxylic ester
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-
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol acetylhydrolase
Involved in the second step of glycosylphosphatidylinositol (GPI) anchor formation in all eukaryotes. The enzyme appears to be composed of a single subunit (PIG-L in mammalian cells and GPI12 in yeast). In some species, the long-chain sn-1-acyl group of the phosphatidyl group is replaced by a long-chain alkyl or alk-1-enyl group.
CAS REGISTRY NUMBER
COMMENTARY
122191-30-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O
D-Glucosaminylphosphatidylinositol + acetate
37°C, pH 7.4, 25 mM KCl, 5 mM MgCl2, 5 mM MnCl2, 0.5 mM dithiothreitol, 0.1 mM 1-chloro-3-tosylamido-7-amino-2-heptanone, 1 microg/ml leupeptin
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-
?
6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate
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-
-
?
6-(N-butanoyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + butanoate
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very poor substrate
-
?
6-(N-hexanoyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + hexanoate
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-
-
?
6-(N-isobutanoyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + isobutanoate
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poor substrate
-
?
6-(N-pentanoyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + pentanoate
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-
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?
6-(N-propionyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + propionate
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-
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?
D-GlcNAc-alpha1-6-D-myo-inositol-1-HPO4-3-sn-1,2-diacylglycerol + H2O
?
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?
N-Acetyl-D-glucosaminylphosphatidylinositol + H2O
D-Glucosaminylphosphatidylinositol + acetate
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?
additional information
?
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no substrate: mannosylated N-acetyl-D-glucosylphosphatidylinositol-derivatives
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?
additional information
?
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structural requirements of substrate, enzyme cannot act on analogues containing N-benzoyl-, N-acetyl-D-galactosyl- or N-acetyl-beta-D-glucosyl-groups
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?
additional information
?
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structural requirements of substrate, enzyme cannot act on substrate analogues containing N-acetyl-beta-glucosyl-groups or aromatic N-acyl groups
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-deoxy-2-ureido-D-glucosyl-alpha1-6-D-myo-inositol-1-phosphoryl-sn-1,2-dipalmitoylglycerol
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50% inhibition between 0.0001 and 0.001 mM
additional information
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not inhibitory up to 0.1 mM: 2-deoxy-2-ureido-D-glucosyl-beta1-6-D-myo-inositol-1-phosphoryl-sn-1,2-dipalmitoylglycerol, 2-deoxy-2-ureido-D-glucosylalpha1-6-D-(2-O-octyl)myo-inositol-1-phosphoryl-sn-1,2-dipalmitoylglycerol
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PIGL_HUMAN
252
1
28531
Swiss-Prot
Secretory Pathway (Reliability: 1)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sharma, D.K.; Smith, T.K.; Crossman, A.; Brimacombe, J.S.; Ferguson, M.A.J.
Substrate specificity of the N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase of glycosylphosphatidylinositol membrane anchor biosynthesis in African trypanosomes and human cells
Biochem. J.
328
171-177
1997
Homo sapiens, Trypanosoma brucei
Smith, T.K.; Crossman, A.; Borissow, C.N.; Paterson, M.J.; Dix, A.; Brimacombe, J.S.; Ferguson, M.A.J.
Specificity of GlcNAc-PI de-N-acetylase of GPI biosynthesis and synthesis of parasite-specific suicide substrate inhibitors
EMBO J.
20
3322-3332
2001
Homo sapiens, Trypanosoma brucei
Smith, T.K.; Crossman, A.; Paterson, M.J.; Borissow, C.N.; Brimacombe, J.S.; Ferguson, M.A.J.
Specificities of enzymes of glycosylphosphatidylinositol biosynthesis in Trypanosoma brucei and HeLa Cells
J. Biol. Chem.
277
37147-37153
2002
Homo sapiens, Trypanosoma brucei
Pielsticker, L.K.; Mann, K.J.; Lin, W.L.; Sevlever, D.
Raft-like membrane domains contain enzymatic activities involved in the synthesis of mammalian glycosylphosphatidylinositol anchor intermediates
Biochem. Biophys. Res. Commun.
330
163-171
2005
Homo sapiens
Pottekat, A.; Menon, A.K.
Subcellular localization and targeting of N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the second enzyme in the glycosylphosphatidylinositol biosynthetic pathway
J. Biol. Chem.
279
15743-15751
2004
Homo sapiens (Q9Y2B2), Homo sapiens
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