Information on EC 3.5.1.83 - N-Acyl-D-aspartate deacylase

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The expected taxonomic range for this enzyme is: Achromobacter xylosoxidans

EC NUMBER
COMMENTARY hide
3.5.1.83
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RECOMMENDED NAME
GeneOntology No.
N-Acyl-D-aspartate deacylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-acyl-D-aspartate + H2O = a carboxylate + D-aspartate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
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SYSTEMATIC NAME
IUBMB Comments
N-Acyl-D-aspartate amidohydrolase
The enzyme from Alcaligenes xylosoxydans subsp. xylosoxydans is specific for N-acyl-D-aspartate. Requires zinc.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-86-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Gly-D-Asp + H2O
Glycine + Asp
show the reaction diagram
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-
-
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N-Acetyl-D-Asp + H2O
acetate + D-Asp
show the reaction diagram
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-
-
-
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N-Butanoyl-D-Asp + H2O
Butanoate + D-Asp
show the reaction diagram
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-
-
-
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N-Formyl-D-Asp + H2O
Formate + D-Asp
show the reaction diagram
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-
-
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N-Propanoyl-D-Asp + H2O
Propanoate + D-Asp
show the reaction diagram
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-
-
-
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additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
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inducible by N-acetyl-D-Asp
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.52
N-Acetyl-D-Asp
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-
0.033
N-Butanoyl-D-Asp
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-
12.5
N-Formyl-D-Asp
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-
1.11
N-Glycyl-D-Asp
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-
0.194
N-Propanoyl-D-Asp
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53581
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1 * 53581, calculation from nucleotide sequence
54000
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1 * 54000, SDS-PAGE
56000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
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stable
31929
8
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stable
31930
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
10 min, stable up to
50
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10 min, 40% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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