Information on EC 3.5.1.76 - Arylalkyl acylamidase

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
3.5.1.76
-
RECOMMENDED NAME
GeneOntology No.
Arylalkyl acylamidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-Acetylarylalkylamine + H2O = arylalkylamine + acetate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
N-Acetylarylalkylamine amidohydrolase
Identified in Pseudomonas putida. Strict specificity for N-acetyl arylalkylamines, including N-acetyl-2-phenylethylamine, N-acetyl-3-phenylpropylamine, N-acetyldopamine, N-acetyl-serotonin and melatonin. It also accepts arylalkyl acetates but not acetanilide derivatives, which are common substrates of EC 3.5.1.13, aryl acylamidase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain Sc2
-
-
Manually annotated by BRENDA team
strain Sc2
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Acetic acid 2-phenylethyl ester + H2O
?
show the reaction diagram
-
-
-
-
-
Acetic acid 3-phenylpropyl ester + H2O
?
show the reaction diagram
-
-
-
-
-
Melatonin + H2O
?
show the reaction diagram
-
-
-
-
-
N-Acetyl-1-methyl-3-phenylpropylamine + H2O
(S)-1-Methyl-3-phenylpropylamine + acetate
show the reaction diagram
N-Acetyl-2-phenylethylamine + H2O
2-Phenylethylamine + acetate
show the reaction diagram
N-Acetyl-3-phenylpropylamine
?
show the reaction diagram
N-Acetylbenzylamine + H2O
?
show the reaction diagram
N-acetyldopamine + H2O
?
show the reaction diagram
-
-
-
-
-
N-Acetylserotonin + H2O
?
show the reaction diagram
-
-
-
-
-
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CdCl2
-
activates
CoCl2
-
activates
CuSO4
-
activates
MgCl2
-
activates
MnCl2
-
activates
NiCl2
-
activates
ZnSO4
-
activates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
2,2'-dipyridyl
-
-
5,5'-dithiobis(2-nitrobenzoic acid)
-
weak
iodoacetate
-
weak
N-bromosuccinimide
-
-
phenylhydrazine
-
-
Semicarbazide
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.9
(R)-N-acetyl-1-methyl-3-phenylpropylamine
-
-
1.5
Melatonin
-
-
0.31
N-acetyl-2-phenylethylamine
-
-
1.6
N-Acetyl-3-phenylpropylamine
-
-
41
N-Acetylbenzylamine
-
-
5
N-acetyldopamine
-
-
0.34
N-acetylserotonin
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
with N-acetyl-2-phenylethylamine as substrate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
-
pH 6.0: about 50% of maximal activity, pH 10: about 40% of maximal activity, with N-acetyl-2-phenylethalamine as substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55 - 75
-
55°C: about 45% of maximal activity, 75°C: about 20% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
-
4 * 42000, SDS-PAGE
42630
-
4 * 42630, SDS-PAGE
150000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
30°C, 30 min, most stable under alkaline conditions
31889
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
pH 7.0, stable below
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE