Information on EC 3.5.1.74 - chenodeoxycholoyltaurine hydrolase

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The expected taxonomic range for this enzyme is: Bacteroides vulgatus

EC NUMBER
COMMENTARY hide
3.5.1.74
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RECOMMENDED NAME
GeneOntology No.
chenodeoxycholoyltaurine hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
chenodeoxycholoyltaurine + H2O = chenodeoxycholate + taurine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
bile acids degradation
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Secondary bile acid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
chenodeoxycholoyltaurine amidohydrolase
Some other taurine conjugates are hydrolysed, but not glycine conjugates of bile acids.
CAS REGISTRY NUMBER
COMMENTARY hide
125752-75-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain VI-31, isolated from human feces
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Manually annotated by BRENDA team
strain VI-31, isolated from human feces
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tauro (3alpha,5beta,7beta,12alpha)-3,7,12-trihydroxycholan-24-oic amide + H2O
taurine + (3alpha,5beta,7beta,12alpha)-3,7,12-trihydroxycholan-24-oic acid
show the reaction diagram
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0.5% of activity compared to taurochenodeoxycholic acid
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-
?
tauro-beta-muricholic acid + H2O
taurine + beta-muricholic acid
show the reaction diagram
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62% of activity compared to taurochenodeoxycholic acid
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-
?
taurochenodeoxycholic acid + H2O
chenodeoxycholate + taurine
show the reaction diagram
taurocholic acid + H2O
taurine + cholic acid
show the reaction diagram
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2.8% of activity compared to taurochenodeoxycholic acid
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-
?
taurodeoxycholic acid + H2O
taurine + deoxycholic acid
show the reaction diagram
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31% of activity compared to taurochenodeoxycholic acid
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-
?
taurohyocholic acid + H2O
taurine + hyocholic acid
show the reaction diagram
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24% of activity compared to taurochenodeoxycholic acid
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?
taurohyodeoxycholic acid + H2O
taurine + hyodeoxycholic acid
show the reaction diagram
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36% of activity compared to taurochenodeoxycholic acid
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?
tauroursodeoxycholic acid + H2O
taurine + ursodeoxycholic acid
show the reaction diagram
-
56% of activity compared to taurochenodeoxycholic acid
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?
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Dithiodipyridine
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0.1 mM: 15.9% inhibition, 1 mM: 50.2% inhibition, 10 mM: complete inhibition
N-ethylmaleimide
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0.1 mM: 3.5% inhibition, 1 mM: 27.4% inhibition, 10 mM: 90.8% inhibition
p-hydroxymercuribenzoate
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0.00001 mM: 4% inhibition, 0.0001 mM: 60% inhibition, 0.001 mM: complete inhibition
sulfhydryl reagents
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
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0.1-1 mM: slight activation, 10 mM: high activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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kinetic properties
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.11
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substrate: tauro-7beta-cholic acid
0.69
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substrate: taurocholic acid
5.86
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substrate: taurohyocholic acid
7.56
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substrate: taurodeoxycholic acid
8.74
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substrate: taurohyodeoxycholic acid
13.68
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substrate: tauroursodeoxycholic acid
15.15
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substrate: tauro-beta-muricholic acid
24.45
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substrate: taurochenodeoxycholic acid
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 6.4
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broad pH-optimum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
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4 * 36000, SDS-PAGE
140000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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48 h stable at
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for 1 month without any loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
strain VI-31
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