Information on EC 3.5.1.73 - carnitinamidase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY hide
3.5.1.73
-
RECOMMENDED NAME
GeneOntology No.
carnitinamidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-carnitinamide + H2O = L-carnitine + NH3
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of linear amides
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-carnitinamide amidohydrolase
Does not act on D-carnitinamide.
CAS REGISTRY NUMBER
COMMENTARY hide
117444-04-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-carnitine amide + H2O
L-carnitine + NH3
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
10 mM, 18% loss of activity
5,5'-dithiobis(2-nitrobenzoate)
-
1 mM, 66% loss of activity
CoCl2
-
10 mM, 24% loss of activity
D-carnitine
-
-
D-Carnitine amide
-
-
EDTA
-
10 mM, 27% loss of activity
FeCl2
-
10 mM, 43% loss of activity
glutathione
-
10 mM, 28% loss of activity
HgCl2
-
1 mM, complete loss of activity
iodoacetate
-
1 mM, 28% loss of activity
KCN
-
10 mM, 12% loss of activity
L-carnitine
-
-
MnCl2
-
10 mM, 24% loss of activity
p-hydroxymercuribenzoate
-
1 mM, 49% loss of activity
phenylmethanesulfonyl fluoride
-
1 mM, 77% loss of activity
ZnCl2
-
1 mM, 94% loss of activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.3 - 11.6
L-Carnitine amide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
328
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
-
pH 6.0: about 40% of maximal activity, pH 10.0: about 35% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 50
-
about 40% of maximal activity at 20°C and at 50°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66000
-
2 * 66000, SDS-PAGE
125000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 66000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9.5
-
30°C, 30 min, less than 20% loss of activity in the pH-range 5.0-9.5
288939
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
in absence of substrate, half-life: 2.6 days
30
-
stable up to
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
during continuous conversion in an enzyme-membrane reactor, the half-life of the catalyst is around 55 hours under unoptimized conditions
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for several months
-
25°C, in absence of substrate, half-life is 2.6 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
the enzyme is a valuable tool for L-carnitine production